LOCUS       BBa_S04074              3252 bp    DNA     linear       23-Dec-2024
DEFINITION  K137106:B0015
ACCESSION   BBa_S04074
VERSION     BBa_S04074.1  
FEATURES             Location/Qualifiers
     CDS             1..3115
                     /label=BBa_K137106
     stem_loop       3135..3178
                     /label=stem_loop
     misc_feature    3124..3203
                     /label=BBa_B0010
     terminator      3124..3203
                     /label=BBa_B0010
     stem_loop       3219..3238
                     /label=T7 TE
     misc_feature    3245..3245
                     /label=stop
     polyA_site      3239..3252
                     /label=polya
     misc_feature    3212..3252
                     /label=BBa_B0012
     terminator      3212..3252
                     /label=BBa_B0012
ORIGIN
        1 atgagtcagt cagtcagtca gtcagtcagt cagtcagtca gtcaccatga ttacggattc
       61 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      121 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      181 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc
      241 agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt
      301 cccctcaaac tggcagatgc acggttacga tgcgcccatc tacaccaacg tgacctatcc
      361 cattacggtc aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac
      421 atttaatgtt gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt
      481 taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg
      541 tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt
      601 gatggtgctg cgctggagtg acggcagtta tctggaagat caggatatgt ggcggatgag
      661 cggcattttc cgtgacgtct cgttgctgca taaaccgact acacaaatca gcgatttcca
      721 tgttgccact cgctttaatg atgatttcag ccgcgctgta ctggaggctg aagttcagat
      781 gtgcggcgag ttgcgtgact acctacgggt aacagtttct ttatggcagg gtgaaacgca
      841 ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc
      901 cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc
      961 gaatctctat cgtgcggtgg ttgaactgca caccgccgac ggcacgctga ttgaagcaga
     1021 agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg
     1081 caagccgttg ctgattcgag gcgttaaccg tcacgagcat catcctctgc atggtcaggt
     1141 catggatgag cagacgatgg tgcaggatat cctgctgatg aagcagaaca actttaacgc
     1201 cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg accgctacgg
     1261 cctgtatgtg gtggatgaag ccaatattga aacccacggc atggtgccaa tgaatcgtct
     1321 gaccgatgat ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg
     1381 cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg aatgaatcag gccacggcgc
     1441 taatcacgac gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc cggtgcagta
     1501 tgaaggcggc ggagccgaca ccacggccac cgatattatt tgcccgatgt acgcgcgcgt
     1561 ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct
     1621 acctggagag acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct
     1681 tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt
     1741 cgtctgggac tgggtggatc agtcgctgat taaatatgat gaaaacggca acccgtggtc
     1801 ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc cagttctgta tgaacggtct
     1861 ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc agcagcagtt
     1921 tttccagttc cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca
     1981 tagcgataac gagctcctgc actggatggt ggcgctggat ggtaagccgc tggcaagcgg
     2041 tgaagtgcct ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc
     2101 gcagccggag agcgccgggc aactctggct cacagtacgc gtagtgcaac cgaacgcgac
     2161 cgcatggtca gaagccgggc acatcagcgc ctggcagcag tggcgtctgg cggaaaacct
     2221 cagtgtgacg ctccccgccg cgtcccacgc catcccgcat ctgaccacca gcgaaatgga
     2281 tttttgcatc gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc
     2341 acagatgtgg attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg
     2401 tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg
     2461 ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac
     2521 ggcagataca cttgctgatg cggtgctgat tacgaccgct cacgcgtggc agcatcaggg
     2581 gaaaacctta tttatcagcc ggaaaaccta ccggattgat ggtagtggtc aaatggcgat
     2641 taccgttgat gttgaagtgg cgagcgatac accgcatccg gcgcggattg gcctgaactg
     2701 ccagctggcg caggtagcag agcgggtaaa ctggctcgga ttagggccgc aagaaaacta
     2761 tcccgaccgc cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta
     2821 taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta
     2881 tggcccacac cagtggcgcg gcgacttcca gttcaacatc agccgctaca gtcaacagca
     2941 actgatggaa accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat
     3001 cgacggtttc catatgggga ttggtggcga cgactcctgg agcccgtcag tatcggcgga
     3061 atttcagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataatacta
     3121 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     3181 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     3241 tctgcgttta ta
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