LOCUS       BBa_S04091              1991 bp    DNA     linear       06-Jul-2024
DEFINITION  S04085:Q04400
ACCESSION   BBa_S04091
VERSION     BBa_S04091.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..83
                     /label=prefix
     misc_feature    255..255
                     /label=T
     misc_feature    573..573
                     /label=A
     CDS             82..831
                     /label=luxR
     misc_feature    82..837
                     /label=BBa_C0062
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             82..837
                     /label=BBa_C0062
     stem_loop       882..925
                     /label=stem_loop
     misc_feature    871..950
                     /label=BBa_B0010
     terminator      871..950
                     /label=BBa_B0010
     stem_loop       966..985
                     /label=T7 TE
     misc_feature    992..992
                     /label=stop
     polyA_site      986..999
                     /label=polya
     misc_feature    959..999
                     /label=BBa_B0012
     terminator      959..999
                     /label=BBa_B0012
     misc_feature    1008..1027
                     /label=LuxR/HSL
     promoter        1027..1032
                     /label=-35
     promoter        1049..1054
                     /label=-10
     misc_feature    1060..1060
                     /label=start
     misc_feature    1008..1062
                     /label=BBa_R0062
     promoter        1008..1062
                     /label=BBa_R0062
     RBS             1071..1081
                     /label=RBS-4\Weaker
     RBS             1077..1080
                     /label=RBS
     misc_feature    1071..1081
                     /label=BBa_B0033
     RBS             1071..1081
                     /label=BBa_B0033
     misc_feature    1094..1097
                     /label=conserved
     RBS             1090..1101
                     /label=BBa_B0034
     CDS             1111..1727
                     /label=tetR
     misc_feature    1728..1761
                     /label=SsrA
     misc_feature    1768..1792
                     /label=Help:Barcodes
     CDS             1108..1767
                     /label=BBa_C0040
     stem_loop       1812..1855
                     /label=stem_loop
     misc_feature    1801..1880
                     /label=BBa_B0010
     terminator      1801..1880
                     /label=BBa_B0010
     stem_loop       1896..1915
                     /label=T7 TE
     misc_feature    1922..1922
                     /label=stop
     polyA_site      1916..1929
                     /label=polya
     misc_feature    1889..1929
                     /label=BBa_B0012
     terminator      1889..1929
                     /label=BBa_B0012
     misc_feature    1938..1956
                     /label=TetR 1
     misc_feature    1938..1991
                     /label=BBa_R0040
     promoter        1957..1962
                     /label=-35
     misc_feature    1963..1981
                     /label=TetR 2
     promoter        1980..1985
                     /label=-10
     promoter        1938..1991
                     /label=BBa_R0040
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt
      121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact
      181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa
      241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct
      301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat
      361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg
      421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc
      481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat
      541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat
      601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg
      661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact
      721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt
      781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac
      841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt
      901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg
     1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag tcacacagga
     1081 ctactagaga aagaggagaa atactagatg tccagattag ataaaagtaa agtgattaac
     1141 agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc
     1201 cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg
     1261 ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa
     1321 ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta
     1381 agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa
     1441 actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta
     1501 tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat
     1561 caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga
     1621 caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa
     1681 ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc tgcaaacgac
     1741 gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc actactagag
     1801 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1861 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1921 gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc agtgatagag
     1981 atactgagca c
//