LOCUS       BBa_S04112              1451 bp    DNA     linear       25-Nov-2024
DEFINITION  J23100:S04108
ACCESSION   BBa_S04112
VERSION     BBa_S04112.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..54
                     /label=RBS-4\Weaker
     RBS             50..53
                     /label=RBS
     misc_feature    44..54
                     /label=BBa_B0033
     RBS             44..54
                     /label=BBa_B0033
     CDS             61..1314
                     /label=BBa_K137001
     stem_loop       1334..1377
                     /label=stem_loop
     misc_feature    1323..1402
                     /label=BBa_B0010
     terminator      1323..1402
                     /label=BBa_B0010
     stem_loop       1418..1437
                     /label=T7 TE
     misc_feature    1444..1444
                     /label=stop
     polyA_site      1438..1451
                     /label=polya
     misc_feature    1411..1451
                     /label=BBa_B0012
     terminator      1411..1451
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagtcacaca ggactactag
       61 atgtactatt taaaaaacac aaacttttgg atgttcggtt tattcttttt cttttacttt
      121 tttatcatgg gagcctactt cccgtttttc ccgatttggc tacatgacat caaccatatc
      181 agcaaaagtg atacgggtat tatttttgcc gctatttctc tgttctcgct attattccaa
      241 ccgctgtttg gtctgctttc tgacaaactc gggctgcgca aatacctgct gtggattatt
      301 accggcatgt tagtgatgtt tgcgccgttc tttattttta tcttcgggcc actgttacaa
      361 tacaacattt tagtaggatc gattgttggt ggtatttatc taggcttttg ttttaacgcc
      421 ggtgcgccag cagtagaggc atttattgag aaagtcagcc gtcgcagtaa tttcgaattt
      481 ggtcgcgcgc ggatgtttgg ctgtgttggc tgggcgctgt gtgcctcgat tgtcggcatc
      541 atgttcacca tcaataatca gtttgttttc tggctgggct ctggctgtgc actcatcctc
      601 gccgttttac tctttttcgc caaaacggat gcgccctctt ctgccacggt tgccaatgcg
      661 gtaggtgcca accattcggc atttagcctt aagctggcac tggaactgtt cagacagcca
      721 aaactgtggt ttttgtcact gtatgttatt ggcgtttcct gcacctacga tgtttttgac
      781 caacagtttg ctaatttctt tacttcgttc tttgctaccg gtgaacaggg tacgcgggta
      841 tttggctacg taacgacaat gggcgaatta cttaacgcct cgattatgtt ctttgcgcca
      901 ctgatcatta atcgcatcgg tgggaaaaac gccctgctgc tggctggcac tattatgtct
      961 gtacgtatta ttggctcatc gttcgccacc tcagcgctgg aagtggttat tctgaaaacg
     1021 ctgcatatgt ttgaagtacc gttcctgctg gtgggctgct ttaaatatat taccagccag
     1081 tttgaagtgc gtttttcagc gacgatttat ctggtctgtt tctgcttctt taagcaactg
     1141 gcgatgattt ttatgtctgt actggcgggc aatatgtatg aaagcatcgg tttccagggc
     1201 gcttatctgg tgctgggtct ggtggcgctg ggcttcacct taatttccgt gttcacgctt
     1261 agcggccccg gcccgctttc cctgctgcgt cgtcaggtga atgaagtcgc ttaatactag
     1321 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1381 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1441 ctgcgtttat a
//