LOCUS       BBa_S04117              1733 bp    DNA     linear       14-May-2024
DEFINITION  Lysis cassette under tight lactose inducible expression.
ACCESSION   BBa_S04117
VERSION     BBa_S04117.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     promoter        138..272
                     /label=BBa_K137127
     misc_feature    1..272
                     /label=BBa_S04115
     misc_feature    281..1596
                     /label=BBa_K137126
     stem_loop       1616..1659
                     /label=stem_loop
     misc_feature    1605..1684
                     /label=BBa_B0010
     terminator      1605..1684
                     /label=BBa_B0010
     stem_loop       1700..1719
                     /label=T7 TE
     misc_feature    1726..1726
                     /label=stop
     polyA_site      1720..1733
                     /label=polya
     misc_feature    1693..1733
                     /label=BBa_B0012
     terminator      1693..1733
                     /label=BBa_B0012
     terminator      1605..1733
                     /label=BBa_B0015
     misc_feature    281..1733
                     /label=BBa_S04114
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagtat atctagagta caacgtcgtg ttagctgcaa ttgtgagcgg
      181 ataacaattg acatagcgga tacttcctga tataattcgt gcaattttta aacctgtagg
      241 atcgtacagg ttactagtag cggccgctgc agtactagag gattgttcta tcagtaatcg
      301 accttattcc taattaaata gagcaaatcc ccttattggg ggtaagacat gaagatgcca
      361 gaaaaacatg acctgttggc cgccattctc gcggcaaagg aacaaggcat cggggcaatc
      421 cttgcgtttg caatggcgta ccttcgcggc agatataatg gcggtgcgtt tacaaaaaca
      481 gtaatcgacg caacgatgtg cgccattatc gcctggttca ttcgtgacct tctcgacttc
      541 gccggactaa gtagcaatct cgcttatata acgagcgtgt ttatcggcta catcggtact
      601 gactcgattg gttcgcttat caaacgcttc gctgctaaaa aagccggagt agaagatggt
      661 agaaatcaat aatcaacgta aggcgttcct cgatatgctg gcgtggtcgg agggaactga
      721 taacggacgt cagaaaacca gaaatcatgg ttatgacgtc attgtaggcg gagagctatt
      781 tactgattac tccgatcacc ctcgcaaact tgtcacgcta aacccaaaac tcaaatcaac
      841 aggcgccgga cgctaccagc ttctttcccg ttggtgggat gcctaccgca agcagcttgg
      901 cctgaaagac ttctctccga aaagtcagga cgctgtggca ttgcagcaga ttaaggagcg
      961 tggcgcttta cctatgattg atcgtggtga tatccgtcag gcaatcgacc gttgcagcaa
     1021 tatctgggct ttactgccgg gcgctggtta tggtcagttc gagcataagg ctgacagcct
     1081 gattgcaaaa ttcaaagaag cgggcggaac ggtcagagag attgatgtat gagcagagtc
     1141 accgcgatta tctccgctct ggttatctgc atcatcgtct gcctgtcatg ggctgttaat
     1201 cattaccgtg ataacgccat tacctacaaa gcccagcgcg acaaaaatgc cagagaactg
     1261 aagctggcga acgcggcaat tactgacatg cagatgcgtc agcgtgatgt tgctgcgctc
     1321 gatgcaaaat acacgaagga gttagctgat gctaaagctg aaaatgatgc tctgcgtgat
     1381 gatgttgccg ctggtcgtcg tcggttgcac atcaaagcag tctgtcagtc agtgcgtgaa
     1441 gccaccaccg cctccggcgt ggataatgca gcctcccccc gactggcaga caccgctgaa
     1501 cgggattatt tcaccctcag agagaggctg atcactatgc aaaaacaact ggaaggaacc
     1561 cagaagtata ttaatgagca gtgcagatag taataatact agagccaggc atcaaataaa
     1621 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1681 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//