LOCUS BBa_S04125 946 bp DNA linear 29-Dec-2024 DEFINITION R0040:I1466 ACCESSION BBa_S04125 VERSION BBa_S04125.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..809 /label=BBa_C0171 stem_loop 829..872 /label=stem_loop misc_feature 818..897 /label=BBa_B0010 terminator 818..897 /label=BBa_B0010 stem_loop 913..932 /label=T7 TE misc_feature 939..939 /label=stop polyA_site 933..946 /label=polya misc_feature 906..946 /label=BBa_B0012 terminator 906..946 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaggaatg acggaggctt tttgctgtgg tgggacggtt 121 tgcgtagcga gatgcagccg atccacgaca gccagggcgt gttcgccgtc ctggaaaagg 181 aagtgcggcg cctgggcttc gattactacg cctatggcgt gcgccacacg attcccttca 241 cccggccgaa gaccgaggtc catggcacct atcccaaggc ctggctggag cgataccaga 301 tgcagaacta cggggccgtg gatccggcga tcctcaacgg cctgcgctcc tcggaaatgg 361 tggtctggag cgacagcctg ttcgaccaga gccggatgct ctggaacgag gctcgcgatt 421 ggggcctctg tgtcggcgcg accttgccga tccgcgcgcc gaacaatttg ctcagcgtgc 481 tttccgtggc gcgcgaccag cagaacatct ccagcttcga gcgcgaggaa atccgcctgc 541 ggctgcgttg catgatcgag ttgctgaccc agaagctgac cgacctggag catccgatgc 601 tgatgtccaa cccggtctgc ctgagccatc gcgaacgcga gatcctgcaa tggaccgccg 661 acggcaagag ttccggggaa atcgccatca tcctgagcat ctccgagagc acggtgaact 721 tccaccacaa gaacatccag aagaagttcg acgcgccgaa caagacgctg gctgccgcct 781 acgccgcggc gctgggtctc atctaataat actagagcca ggcatcaaat aaaacgaaag 841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 901 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //