BBa_I725013
1
BBa_I725013
Butyryl CoA dehyrogenase
2007-06-17T11:00:00Z
2015-08-31T04:07:54Z
C. acetobutylicum genom. CAC_2712
Butyryl coa dehyrogenase from C. acetobutylicum. The sequence has been selected because it is part of an operon that has successfully been cloned into E. coli. It catalyzes the third step in butanol biosythesis. It has a His-tag added to its C terminus.
false
false
_106_
0
1824
9
It's complicated
false
The sequence has been optimized for use in E. Coli and has had its restriction sites removed. It has had a c-terminal His tag added and its stop codon has been changed to TAATAA.
false
Erin Dul
BBa_S04143
1
BBa_S04143
The Partial Butanol Operon (Parts I725023+I725024)
2008-10-28T12:00:00Z
2015-05-08T01:14:35Z
false
false
_241_
0
2745
9
It's complicated
false
false
Tom Tran
component1993620
1
BBa_I725013
component1993623
1
BBa_I725014
component1993619
1
BBa_B0034
component1993622
1
BBa_B0034
annotation1993623
1
BBa_I725014
range1993623
1
1200
3797
annotation1993619
1
BBa_B0034
range1993619
1
1
12
annotation1993620
1
BBa_I725013
range1993620
1
19
1173
annotation1993622
1
BBa_B0034
range1993622
1
1182
1193
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_I725014
1
BBa_I725014
Butyraldehyde dehydrogenase
2007-06-17T11:00:00Z
2015-08-31T04:07:54Z
pSOL plamid of C. acetobutylicum. CAP_0035
This is the butyraldehyde dehydrogenase from C. acetobutylicum. It catalyzes the fourth step in the reaction of butanol biosythesis. Its is from the pSOL plasmid of C. acetobutylicum. It also has some alcohol dehydrogenase activity.
false
false
_106_
0
1824
9
It's complicated
false
The sequence has been optimized for use in E. Coli and has had its restriction sites removed. It has had a c-terminal His tag added and its stop codon has been changed to TAATAA.
false
Erin Dul
BBa_I725014_sequence
1
atgaaagttaccaaccagaaagaactgaaacagaaactgaacgaactgcgtgaagctcagaaaaaattcgctacctacacccaggaacaggttgacaaaatcttcaaacagtgcgctatcgctgctgctaaagaacgtatcaacctggctaaactggctgttgaagaaaccggtatcggtctggttgaagacaaaatcatcaaaaaccacttcgctgctgaatacatctacaacaaatacaaaaacgaaaaaacctgcggtatcatcgaccacgacgactccctgggtatcaccaaagttgctgaaccgatcggtatcgttgctgctatcgttccgaccaccaacccgacctccaccgctatcttcaaatccctgatctccctgaaaacccgtaacgctatcttcttctccccgcacccgcgtgctaaaaaatccaccatcgctgctgctaaactgatcctggacgctgctgttaaagctggtgctccgaaaaacatcatcggttggatcgacgaaccgtccatcgaactgtcccaggacctgatgtccgaagctgacatcatcctggctaccggtggtccgtccatggttaaagctgcttactcctccggtaaaccggctatcggtgttggtgctggtaacaccccggctatcatcgacgaatccgctgacatcgacatggctgtttcctccatcatcctgtccaaaacctacgacaacggtgttatctgcgcttccgaacagtccatcctggttatgaactccatctacgaaaaagttaaagaagagttcgttaaacgtggttcctacatcctgaaccagaacgaaatcgctaaaatcaaagaaaccatgttcaaaaacggtgctatcaacgctgacatcgttggtaaatccgcttacatcatcgctaaaatggctggtatcgaagttccgcagaccaccaaaatcctgatcggtgaagttcagtccgttgaaaaatccgaactgttctcccacgaaaaactgtccccggttctggctatgtacaaagttaaagacttcgacgaagctctgaaaaaagctcagcgtctgatcgaactgggtggttccggtcacacctcctccctgtacatcgactcccagaacaacaaagacaaagttaaagagttcggtctggctatgaaaacctcccgtaccttcatcaacatgccgtcctcccagggtgcttccggtgacctgtacaacttcgctatcgctccgtccttcaccctgggttgcggtacctggggtggtaactccgtttcccagaacgttgaaccgaaacacctgctgaacatcaaatccgttgctgaacgtcgtgaaaacatgctgtggttcaaagttccgcagaaaatctacttcaaatacggttgcctgcgtttcgctctgaaagaactgaaagacatgaacaaaaaacgtgctttcatcgttaccgacaaagacctgttcaaactgggttacgttaacaaaatcaccaaagttctggacgaaatcgacatcaaatactccatcttcaccgacatcaaatccgacccgaccatcgactccgttaaaaaaggtgctaaagaaatgctgaacttcgaaccggacaccatcatctccatcggtggtggttccccgatggacgctgctaaagttatgcacctgctgtacgaatacccggaagctgaaatcgaaaacctggctatcaacttcatggacatccgtaaacgtatctgcaacttcccgaaactgggtaccaaagctatctccgttgctatcccgaccaccgctggtaccggttccgaagctaccccgttcgctgttatcaccaacgacgaaaccggtatgaaatacccgctgacctcctacgaactgaccccgaacatggctatcatcgacaccgaactgatgctgaacatgccgcgtaaactgaccgctgctaccggtatcgacgctctggttcacgctatcgaagcttacgtttccgttatggctaccgactacaccgacgaactggctctgcgtgctatcaaaatgatcttcaaatacctgccgcgtgcttacaaaaacggtaccaacgacatcgaagctcgtgaaaaaatggctcacgcttccaacatcgctggtatggctttcgctaacgctttcctgggtgtttgccactccatggctcacaaactgggtgctatgcaccacgttccgcacggtatcgcttgcgctgttctgatcgaagaagttatcaaatacaacgctaccgactgcccgaccaaacagaccgctttcccgcagtacaaatccccgaacgctaaacgtaaatacgctgaaatcgctgaatacctgaacctgaaaggtacctccgacaccgaaaaagttaccgctctgatcgaagctatctccaaactgaaaatcgacctgtccatcccgcagaacatctccgctgctggtatcaacaaaaaagacttctacaacaccctggacaaaatgtccgaactggctttcgacgaccagtgcaccaccgctaacccgcgttacccgctgatctccgaactgaaagacatctacatcaaatccttccatcaccatcaccatcactaataa
BBa_I725013_sequence
1
atggacttcaacctgacccgtgaacaggaactggttcgtcagatggttcgtgagttcgctgaaaacgaagttaaaccgatcgctgctgaaatcgacgaaaccgaacgtttcccgatggaaaacgttaaaaaaatgggtcagtacggtatgatgggtatcccgttctccaaagaatacggtggtgctggtggtgacgttctgtcctacatcatcgctgttgaagaactgtccaaagtttgcggtaccaccggtgttatcctgtccgctcacacctccctgtgcgcttccctgatcaacgaacacggtaccgaagaacagaaacagaaatacctggttccgctggctaaaggtgaaaaaatcggtgcttacggtctgaccgaaccgaacgctggtaccgactccggtgctcagcagaccgttgctgttctggaaggtgaccactacgttatcaacggttccaaaatcttcatcaccaacggtggtgttgctgacaccttcgttatcttcgctatgaccgaccgtaccaaaggtaccaaaggtatctccgctttcatcatcgaaaaaggtttcaaaggtttctccatcggtaaagttgaacagaaactgggtatccgtgcttcctccaccaccgaactggttttcgaagacatgatcgttccggttgaaaacatgatcggtaaagaaggtaaaggtttcccgatcgctatgaaaaccctggacggtggtcgtatcggtatcgctgctcaggctctgggtatcgctgaaggtgctttcaacgaagctcgtgcttacatgaaagaacgtaaacagttcggtcgttccctggacaaattccagggtctggcttggatgatggctgacatggacgttgctatcgaatccgctcgttacctggtttacaaagctgcttacctgaaacaggctggtctgccgtacaccgttgacgctgctcgtgctaaactgcacgctgctaacgttgctatggacgttaccaccaaagctgttcagctgttcggtggttacggttacaccaaagactacccggttgaacgtatgatgcgtgacgctaaaatcaccgaaatctacgaaggtacctccgaagttcagaaactggttatctccggtaaaatcttccgtcatcaccatcaccatcac
BBa_B0034_sequence
1
aaagaggagaaa
BBa_S04143_sequence
1
aaagaggagaaatactagatggacttcaacctgacccgtgaacaggaactggttcgtcagatggttcgtgagttcgctgaaaacgaagttaaaccgatcgctgctgaaatcgacgaaaccgaacgtttcccgatggaaaacgttaaaaaaatgggtcagtacggtatgatgggtatcccgttctccaaagaatacggtggtgctggtggtgacgttctgtcctacatcatcgctgttgaagaactgtccaaagtttgcggtaccaccggtgttatcctgtccgctcacacctccctgtgcgcttccctgatcaacgaacacggtaccgaagaacagaaacagaaatacctggttccgctggctaaaggtgaaaaaatcggtgcttacggtctgaccgaaccgaacgctggtaccgactccggtgctcagcagaccgttgctgttctggaaggtgaccactacgttatcaacggttccaaaatcttcatcaccaacggtggtgttgctgacaccttcgttatcttcgctatgaccgaccgtaccaaaggtaccaaaggtatctccgctttcatcatcgaaaaaggtttcaaaggtttctccatcggtaaagttgaacagaaactgggtatccgtgcttcctccaccaccgaactggttttcgaagacatgatcgttccggttgaaaacatgatcggtaaagaaggtaaaggtttcccgatcgctatgaaaaccctggacggtggtcgtatcggtatcgctgctcaggctctgggtatcgctgaaggtgctttcaacgaagctcgtgcttacatgaaagaacgtaaacagttcggtcgttccctggacaaattccagggtctggcttggatgatggctgacatggacgttgctatcgaatccgctcgttacctggtttacaaagctgcttacctgaaacaggctggtctgccgtacaccgttgacgctgctcgtgctaaactgcacgctgctaacgttgctatggacgttaccaccaaagctgttcagctgttcggtggttacggttacaccaaagactacccggttgaacgtatgatgcgtgacgctaaaatcaccgaaatctacgaaggtacctccgaagttcagaaactggttatctccggtaaaatcttccgtcatcaccatcaccatcactactagagaaagaggagaaatactagatgaaagttaccaaccagaaagaactgaaacagaaactgaacgaactgcgtgaagctcagaaaaaattcgctacctacacccaggaacaggttgacaaaatcttcaaacagtgcgctatcgctgctgctaaagaacgtatcaacctggctaaactggctgttgaagaaaccggtatcggtctggttgaagacaaaatcatcaaaaaccacttcgctgctgaatacatctacaacaaatacaaaaacgaaaaaacctgcggtatcatcgaccacgacgactccctgggtatcaccaaagttgctgaaccgatcggtatcgttgctgctatcgttccgaccaccaacccgacctccaccgctatcttcaaatccctgatctccctgaaaacccgtaacgctatcttcttctccccgcacccgcgtgctaaaaaatccaccatcgctgctgctaaactgatcctggacgctgctgttaaagctggtgctccgaaaaacatcatcggttggatcgacgaaccgtccatcgaactgtcccaggacctgatgtccgaagctgacatcatcctggctaccggtggtccgtccatggttaaagctgcttactcctccggtaaaccggctatcggtgttggtgctggtaacaccccggctatcatcgacgaatccgctgacatcgacatggctgtttcctccatcatcctgtccaaaacctacgacaacggtgttatctgcgcttccgaacagtccatcctggttatgaactccatctacgaaaaagttaaagaagagttcgttaaacgtggttcctacatcctgaaccagaacgaaatcgctaaaatcaaagaaaccatgttcaaaaacggtgctatcaacgctgacatcgttggtaaatccgcttacatcatcgctaaaatggctggtatcgaagttccgcagaccaccaaaatcctgatcggtgaagttcagtccgttgaaaaatccgaactgttctcccacgaaaaactgtccccggttctggctatgtacaaagttaaagacttcgacgaagctctgaaaaaagctcagcgtctgatcgaactgggtggttccggtcacacctcctccctgtacatcgactcccagaacaacaaagacaaagttaaagagttcggtctggctatgaaaacctcccgtaccttcatcaacatgccgtcctcccagggtgcttccggtgacctgtacaacttcgctatcgctccgtccttcaccctgggttgcggtacctggggtggtaactccgtttcccagaacgttgaaccgaaacacctgctgaacatcaaatccgttgctgaacgtcgtgaaaacatgctgtggttcaaagttccgcagaaaatctacttcaaatacggttgcctgcgtttcgctctgaaagaactgaaagacatgaacaaaaaacgtgctttcatcgttaccgacaaagacctgttcaaactgggttacgttaacaaaatcaccaaagttctggacgaaatcgacatcaaatactccatcttcaccgacatcaaatccgacccgaccatcgactccgttaaaaaaggtgctaaagaaatgctgaacttcgaaccggacaccatcatctccatcggtggtggttccccgatggacgctgctaaagttatgcacctgctgtacgaatacccggaagctgaaatcgaaaacctggctatcaacttcatggacatccgtaaacgtatctgcaacttcccgaaactgggtaccaaagctatctccgttgctatcccgaccaccgctggtaccggttccgaagctaccccgttcgctgttatcaccaacgacgaaaccggtatgaaatacccgctgacctcctacgaactgaccccgaacatggctatcatcgacaccgaactgatgctgaacatgccgcgtaaactgaccgctgctaccggtatcgacgctctggttcacgctatcgaagcttacgtttccgttatggctaccgactacaccgacgaactggctctgcgtgctatcaaaatgatcttcaaatacctgccgcgtgcttacaaaaacggtaccaacgacatcgaagctcgtgaaaaaatggctcacgcttccaacatcgctggtatggctttcgctaacgctttcctgggtgtttgccactccatggctcacaaactgggtgctatgcaccacgttccgcacggtatcgcttgcgctgttctgatcgaagaagttatcaaatacaacgctaccgactgcccgaccaaacagaccgctttcccgcagtacaaatccccgaacgctaaacgtaaatacgctgaaatcgctgaatacctgaacctgaaaggtacctccgacaccgaaaaagttaccgctctgatcgaagctatctccaaactgaaaatcgacctgtccatcccgcagaacatctccgctgctggtatcaacaaaaaagacttctacaacaccctggacaaaatgtccgaactggctttcgacgaccagtgcaccaccgctaacccgcgttacccgctgatctccgaactgaaagacatctacatcaaatccttccatcaccatcaccatcactaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z