BBa_S04144 1 BBa_S04144 The Partial Butanol Operon (Parts I725024+I725025) 2008-10-28T12:00:00Z 2015-05-08T01:14:35Z false false _241_ 0 2745 9 It's complicated false false Tom Tran component1993629 1 BBa_I725015 component1993625 1 BBa_B0034 component1993626 1 BBa_I725014 component1993628 1 BBa_B0034 annotation1993629 1 BBa_I725015 range1993629 1 2643 3830 annotation1993625 1 BBa_B0034 range1993625 1 1 12 annotation1993628 1 BBa_B0034 range1993628 1 2625 2636 annotation1993626 1 BBa_I725014 range1993626 1 19 2616 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I725014 1 BBa_I725014 Butyraldehyde dehydrogenase 2007-06-17T11:00:00Z 2015-08-31T04:07:54Z pSOL plamid of C. acetobutylicum. CAP_0035 This is the butyraldehyde dehydrogenase from C. acetobutylicum. It catalyzes the fourth step in the reaction of butanol biosythesis. Its is from the pSOL plasmid of C. acetobutylicum. It also has some alcohol dehydrogenase activity. false false _106_ 0 1824 9 It's complicated false The sequence has been optimized for use in E. Coli and has had its restriction sites removed. It has had a c-terminal His tag added and its stop codon has been changed to TAATAA. false Erin Dul BBa_I725015 1 BBa_I725015 Butanol dehydrogenase 2007-06-17T11:00:00Z 2015-08-31T04:07:54Z C. acetobutylicum genome CAC_3298 Butanol dehydrogenase from C. acetobutylicum. This catalyzes the fifth step of butanol biosythesis. It has been cloned into e. coli previously and activity has been recovered. false false _106_ 0 1824 9 It's complicated false This part has been optimized for synthesis in E. Coli and has had its restriction sites removed. It has had its GTG start codon mutated to ATG and its stop codon mutated to TAATAA. It has also had ad C terminal His tag added. false Erin Dul BBa_I725014_sequence 1 atgaaagttaccaaccagaaagaactgaaacagaaactgaacgaactgcgtgaagctcagaaaaaattcgctacctacacccaggaacaggttgacaaaatcttcaaacagtgcgctatcgctgctgctaaagaacgtatcaacctggctaaactggctgttgaagaaaccggtatcggtctggttgaagacaaaatcatcaaaaaccacttcgctgctgaatacatctacaacaaatacaaaaacgaaaaaacctgcggtatcatcgaccacgacgactccctgggtatcaccaaagttgctgaaccgatcggtatcgttgctgctatcgttccgaccaccaacccgacctccaccgctatcttcaaatccctgatctccctgaaaacccgtaacgctatcttcttctccccgcacccgcgtgctaaaaaatccaccatcgctgctgctaaactgatcctggacgctgctgttaaagctggtgctccgaaaaacatcatcggttggatcgacgaaccgtccatcgaactgtcccaggacctgatgtccgaagctgacatcatcctggctaccggtggtccgtccatggttaaagctgcttactcctccggtaaaccggctatcggtgttggtgctggtaacaccccggctatcatcgacgaatccgctgacatcgacatggctgtttcctccatcatcctgtccaaaacctacgacaacggtgttatctgcgcttccgaacagtccatcctggttatgaactccatctacgaaaaagttaaagaagagttcgttaaacgtggttcctacatcctgaaccagaacgaaatcgctaaaatcaaagaaaccatgttcaaaaacggtgctatcaacgctgacatcgttggtaaatccgcttacatcatcgctaaaatggctggtatcgaagttccgcagaccaccaaaatcctgatcggtgaagttcagtccgttgaaaaatccgaactgttctcccacgaaaaactgtccccggttctggctatgtacaaagttaaagacttcgacgaagctctgaaaaaagctcagcgtctgatcgaactgggtggttccggtcacacctcctccctgtacatcgactcccagaacaacaaagacaaagttaaagagttcggtctggctatgaaaacctcccgtaccttcatcaacatgccgtcctcccagggtgcttccggtgacctgtacaacttcgctatcgctccgtccttcaccctgggttgcggtacctggggtggtaactccgtttcccagaacgttgaaccgaaacacctgctgaacatcaaatccgttgctgaacgtcgtgaaaacatgctgtggttcaaagttccgcagaaaatctacttcaaatacggttgcctgcgtttcgctctgaaagaactgaaagacatgaacaaaaaacgtgctttcatcgttaccgacaaagacctgttcaaactgggttacgttaacaaaatcaccaaagttctggacgaaatcgacatcaaatactccatcttcaccgacatcaaatccgacccgaccatcgactccgttaaaaaaggtgctaaagaaatgctgaacttcgaaccggacaccatcatctccatcggtggtggttccccgatggacgctgctaaagttatgcacctgctgtacgaatacccggaagctgaaatcgaaaacctggctatcaacttcatggacatccgtaaacgtatctgcaacttcccgaaactgggtaccaaagctatctccgttgctatcccgaccaccgctggtaccggttccgaagctaccccgttcgctgttatcaccaacgacgaaaccggtatgaaatacccgctgacctcctacgaactgaccccgaacatggctatcatcgacaccgaactgatgctgaacatgccgcgtaaactgaccgctgctaccggtatcgacgctctggttcacgctatcgaagcttacgtttccgttatggctaccgactacaccgacgaactggctctgcgtgctatcaaaatgatcttcaaatacctgccgcgtgcttacaaaaacggtaccaacgacatcgaagctcgtgaaaaaatggctcacgcttccaacatcgctggtatggctttcgctaacgctttcctgggtgtttgccactccatggctcacaaactgggtgctatgcaccacgttccgcacggtatcgcttgcgctgttctgatcgaagaagttatcaaatacaacgctaccgactgcccgaccaaacagaccgctttcccgcagtacaaatccccgaacgctaaacgtaaatacgctgaaatcgctgaatacctgaacctgaaaggtacctccgacaccgaaaaagttaccgctctgatcgaagctatctccaaactgaaaatcgacctgtccatcccgcagaacatctccgctgctggtatcaacaaaaaagacttctacaacaccctggacaaaatgtccgaactggctttcgacgaccagtgcaccaccgctaacccgcgttacccgctgatctccgaactgaaagacatctacatcaaatccttccatcaccatcaccatcactaataa BBa_I725015_sequence 1 atggttgacttcgaatactccatcccgacccgtatcttcttcggtaaagacaaaatcaacgttctgggtcgtgaactgaaaaaatacggttccaaagttctgatcgtttacggtggtggttccatcaaacgtaacggtatctacgacaaagctgtttccatcctggaaaaaaactccatcaaattctacgaactggctggtgttgaaccgaacccgcgtgttaccaccgttgaaaaaggtgttaaaatctgccgtgaaaacggtgttgaagttgttctggctatcggtggtggttccgctatcgactgcgctaaagttatcgctgctgcttgcgaatacgacggtaacccgtgggacatcgttctggacggttccaaaatcaaacgtgttctgccgatcgcttccatcctgaccatcgctgctaccggttccgaaatggacacctgggctgttatcaacaacatggacaccaacgaaaaactgatcgctgctcacccggacatggctccgaaattctccatcctggacccgacctacacctacaccgttccgaccaaccagaccgctgctggtaccgctgacatcatgtcccacatcttcgaagtttacttctccaacaccaaaaccgcttacctgcaagaccgtatggctgaagctctgctgcgtacctgcatcaaatacggtggtatcgctctggaaaaaccggacgactacgaagctcgtgctaacctgatgtgggcttcctccctggctatcaacggtctgctgacctacggtaaagacaccaactggtccgttcacctgatggaacacgaactgtccgcttactacgacatcacccacggtgttggtctggctatcctgaccccgaactggatggaatacatcctgaacaacgacaccgtttacaaattcgttgaatacggtgttaacgtttggggtatcgacaaagaaaaaaaccactacgacatcgctcaccaggctatccagaaaacccgtgactacttcgttaacgttctgggtctgccgtcccgtctgcgtgacgttggtatcgaagaagaaaaactggacatcatggctaaagaatccgttaaactgaccggtggtaccatcggtaacctgcgtccggttaacgcttccgaagttctgcaaatcttcaaaaaatccgttcatcaccatcaccatcac BBa_B0034_sequence 1 aaagaggagaaa BBa_S04144_sequence 1 aaagaggagaaatactagatgaaagttaccaaccagaaagaactgaaacagaaactgaacgaactgcgtgaagctcagaaaaaattcgctacctacacccaggaacaggttgacaaaatcttcaaacagtgcgctatcgctgctgctaaagaacgtatcaacctggctaaactggctgttgaagaaaccggtatcggtctggttgaagacaaaatcatcaaaaaccacttcgctgctgaatacatctacaacaaatacaaaaacgaaaaaacctgcggtatcatcgaccacgacgactccctgggtatcaccaaagttgctgaaccgatcggtatcgttgctgctatcgttccgaccaccaacccgacctccaccgctatcttcaaatccctgatctccctgaaaacccgtaacgctatcttcttctccccgcacccgcgtgctaaaaaatccaccatcgctgctgctaaactgatcctggacgctgctgttaaagctggtgctccgaaaaacatcatcggttggatcgacgaaccgtccatcgaactgtcccaggacctgatgtccgaagctgacatcatcctggctaccggtggtccgtccatggttaaagctgcttactcctccggtaaaccggctatcggtgttggtgctggtaacaccccggctatcatcgacgaatccgctgacatcgacatggctgtttcctccatcatcctgtccaaaacctacgacaacggtgttatctgcgcttccgaacagtccatcctggttatgaactccatctacgaaaaagttaaagaagagttcgttaaacgtggttcctacatcctgaaccagaacgaaatcgctaaaatcaaagaaaccatgttcaaaaacggtgctatcaacgctgacatcgttggtaaatccgcttacatcatcgctaaaatggctggtatcgaagttccgcagaccaccaaaatcctgatcggtgaagttcagtccgttgaaaaatccgaactgttctcccacgaaaaactgtccccggttctggctatgtacaaagttaaagacttcgacgaagctctgaaaaaagctcagcgtctgatcgaactgggtggttccggtcacacctcctccctgtacatcgactcccagaacaacaaagacaaagttaaagagttcggtctggctatgaaaacctcccgtaccttcatcaacatgccgtcctcccagggtgcttccggtgacctgtacaacttcgctatcgctccgtccttcaccctgggttgcggtacctggggtggtaactccgtttcccagaacgttgaaccgaaacacctgctgaacatcaaatccgttgctgaacgtcgtgaaaacatgctgtggttcaaagttccgcagaaaatctacttcaaatacggttgcctgcgtttcgctctgaaagaactgaaagacatgaacaaaaaacgtgctttcatcgttaccgacaaagacctgttcaaactgggttacgttaacaaaatcaccaaagttctggacgaaatcgacatcaaatactccatcttcaccgacatcaaatccgacccgaccatcgactccgttaaaaaaggtgctaaagaaatgctgaacttcgaaccggacaccatcatctccatcggtggtggttccccgatggacgctgctaaagttatgcacctgctgtacgaatacccggaagctgaaatcgaaaacctggctatcaacttcatggacatccgtaaacgtatctgcaacttcccgaaactgggtaccaaagctatctccgttgctatcccgaccaccgctggtaccggttccgaagctaccccgttcgctgttatcaccaacgacgaaaccggtatgaaatacccgctgacctcctacgaactgaccccgaacatggctatcatcgacaccgaactgatgctgaacatgccgcgtaaactgaccgctgctaccggtatcgacgctctggttcacgctatcgaagcttacgtttccgttatggctaccgactacaccgacgaactggctctgcgtgctatcaaaatgatcttcaaatacctgccgcgtgcttacaaaaacggtaccaacgacatcgaagctcgtgaaaaaatggctcacgcttccaacatcgctggtatggctttcgctaacgctttcctgggtgtttgccactccatggctcacaaactgggtgctatgcaccacgttccgcacggtatcgcttgcgctgttctgatcgaagaagttatcaaatacaacgctaccgactgcccgaccaaacagaccgctttcccgcagtacaaatccccgaacgctaaacgtaaatacgctgaaatcgctgaatacctgaacctgaaaggtacctccgacaccgaaaaagttaccgctctgatcgaagctatctccaaactgaaaatcgacctgtccatcccgcagaacatctccgctgctggtatcaacaaaaaagacttctacaacaccctggacaaaatgtccgaactggctttcgacgaccagtgcaccaccgctaacccgcgttacccgctgatctccgaactgaaagacatctacatcaaatccttccatcaccatcaccatcactaataatactagagaaagaggagaaatactagatggttgacttcgaatactccatcccgacccgtatcttcttcggtaaagacaaaatcaacgttctgggtcgtgaactgaaaaaatacggttccaaagttctgatcgtttacggtggtggttccatcaaacgtaacggtatctacgacaaagctgtttccatcctggaaaaaaactccatcaaattctacgaactggctggtgttgaaccgaacccgcgtgttaccaccgttgaaaaaggtgttaaaatctgccgtgaaaacggtgttgaagttgttctggctatcggtggtggttccgctatcgactgcgctaaagttatcgctgctgcttgcgaatacgacggtaacccgtgggacatcgttctggacggttccaaaatcaaacgtgttctgccgatcgcttccatcctgaccatcgctgctaccggttccgaaatggacacctgggctgttatcaacaacatggacaccaacgaaaaactgatcgctgctcacccggacatggctccgaaattctccatcctggacccgacctacacctacaccgttccgaccaaccagaccgctgctggtaccgctgacatcatgtcccacatcttcgaagtttacttctccaacaccaaaaccgcttacctgcaagaccgtatggctgaagctctgctgcgtacctgcatcaaatacggtggtatcgctctggaaaaaccggacgactacgaagctcgtgctaacctgatgtgggcttcctccctggctatcaacggtctgctgacctacggtaaagacaccaactggtccgttcacctgatggaacacgaactgtccgcttactacgacatcacccacggtgttggtctggctatcctgaccccgaactggatggaatacatcctgaacaacgacaccgtttacaaattcgttgaatacggtgttaacgtttggggtatcgacaaagaaaaaaaccactacgacatcgctcaccaggctatccagaaaacccgtgactacttcgttaacgttctgggtctgccgtcccgtctgcgtgacgttggtatcgaagaagaaaaactggacatcatggctaaagaatccgttaaactgaccggtggtaccatcggtaacctgcgtccggttaacgcttccgaagttctgcaaatcttcaaaaaatccgttcatcaccatcaccatcac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z