BBa_S04145 1 BBa_S04145 The Partial Butanol Operon (Parts I725021+I725022+I725023) 2008-10-28T12:00:00Z 2015-05-08T01:14:35Z false false _241_ 0 2745 9 It's complicated false false Tom Tran component1993634 1 BBa_B0034 component1993631 1 BBa_B0034 component1993637 1 BBa_B0034 component1993635 1 BBa_I72512 component1993638 1 BBa_I725013 component1993632 1 BBa_I725011 annotation1993632 1 BBa_I725011 range1993632 1 19 888 annotation1993634 1 BBa_B0034 range1993634 1 897 908 annotation1993638 1 BBa_I725013 range1993638 1 1742 2896 annotation1993635 1 BBa_I72512 range1993635 1 915 1715 annotation1993637 1 BBa_B0034 range1993637 1 1724 1735 annotation1993631 1 BBa_B0034 range1993631 1 1 12 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I725013 1 BBa_I725013 Butyryl CoA dehyrogenase 2007-06-17T11:00:00Z 2015-08-31T04:07:54Z C. acetobutylicum genom. CAC_2712 Butyryl coa dehyrogenase from C. acetobutylicum. The sequence has been selected because it is part of an operon that has successfully been cloned into E. coli. It catalyzes the third step in butanol biosythesis. It has a His-tag added to its C terminus. false false _106_ 0 1824 9 It's complicated false The sequence has been optimized for use in E. Coli and has had its restriction sites removed. It has had a c-terminal His tag added and its stop codon has been changed to TAATAA. false Erin Dul BBa_I725011 1 BBa_I725011 B-hydroxy butyryl coA dehydrogenase 2007-06-17T11:00:00Z 2015-08-31T04:07:54Z Genomic sequence of Chlostridium acetobutylicum. CAC_2708. B-hydroxybutyryl coa dehydrogenase from C. acetobutylicum. Catalyzes the first step in the conversion of aceto-acetylcoa into butanol. It is from an operon containing this gene as well as two other genes in the butanol production pathway. It has been successfully cloned into e. coli before and activity has been recovered. The sequence has C-terminal His-tag. false false _106_ 0 1824 9 It's complicated false The sequence has been optimized for E. Coli and has had its restriction sites removed. A His tag has been added to the C terminus. The stop codon has been changed to TAATAA. true Erin Dul BBa_I72512 1 BBa_I72512 Enoyl-coa hydratase 2007-06-17T11:00:00Z 2015-08-31T04:07:54Z C. acetobutylicum genome. CAC_2712 Enoly-coa hydratase from C. acetobutylicum. The sequence has been selected because it is part of an operon that has successfully been cloned into E. coli. It catalyzes the second step in butanol biosythesis. It has a His-tag added to its C terminus. false false _106_ 0 1824 9 It's complicated false The sequence has had its codons optimized for E. coli and has had its restriction sites removed. The sequence has had its stop codon changed to TAATAA. It has had a His tag added to its c terminus. true Erin Dul BBa_I725013_sequence 1 atggacttcaacctgacccgtgaacaggaactggttcgtcagatggttcgtgagttcgctgaaaacgaagttaaaccgatcgctgctgaaatcgacgaaaccgaacgtttcccgatggaaaacgttaaaaaaatgggtcagtacggtatgatgggtatcccgttctccaaagaatacggtggtgctggtggtgacgttctgtcctacatcatcgctgttgaagaactgtccaaagtttgcggtaccaccggtgttatcctgtccgctcacacctccctgtgcgcttccctgatcaacgaacacggtaccgaagaacagaaacagaaatacctggttccgctggctaaaggtgaaaaaatcggtgcttacggtctgaccgaaccgaacgctggtaccgactccggtgctcagcagaccgttgctgttctggaaggtgaccactacgttatcaacggttccaaaatcttcatcaccaacggtggtgttgctgacaccttcgttatcttcgctatgaccgaccgtaccaaaggtaccaaaggtatctccgctttcatcatcgaaaaaggtttcaaaggtttctccatcggtaaagttgaacagaaactgggtatccgtgcttcctccaccaccgaactggttttcgaagacatgatcgttccggttgaaaacatgatcggtaaagaaggtaaaggtttcccgatcgctatgaaaaccctggacggtggtcgtatcggtatcgctgctcaggctctgggtatcgctgaaggtgctttcaacgaagctcgtgcttacatgaaagaacgtaaacagttcggtcgttccctggacaaattccagggtctggcttggatgatggctgacatggacgttgctatcgaatccgctcgttacctggtttacaaagctgcttacctgaaacaggctggtctgccgtacaccgttgacgctgctcgtgctaaactgcacgctgctaacgttgctatggacgttaccaccaaagctgttcagctgttcggtggttacggttacaccaaagactacccggttgaacgtatgatgcgtgacgctaaaatcaccgaaatctacgaaggtacctccgaagttcagaaactggttatctccggtaaaatcttccgtcatcaccatcaccatcac BBa_B0034_sequence 1 aaagaggagaaa BBa_I725011_sequence 1 atgaaaaaagtttgcgttatcggtgctggtaccatgggttccggtatcgctcaggctttcgctgctaaaggtttcgaagttgttctgcgtgacatcaaagacgaatttgttgaccgtggtctggacttcatcaacaaaaacctgtccaaactggttaaaaaaggtaaaatcgaagaagctaccaaagttgaaatcctgacccgtatctccggtaccgttgacctgaacatggctgctgactgcgacctggttatcgaagctgctgttgaacgtatggacatcaaaaaacagatcttcgctgacctggacaacatctgcaaaccggaaaccatcctggcttccaacacctcctccctgtccatcaccgaagttgcttccgctaccaaacgtccggacaaagttatcggtatgcacttcttcaacccggctccggttatgaaactggttgaagttatccgtggtatcgctacctcccaggaaaccttcgacgctgttaaagaaacctccatcgctatcggtaaagacccggttgaagttgctgaagctccgggtttcgttgttaaccgtatcctgatcccgatgatcaacgaagctgttggtatcctggctgaaggtatcgcttccgttgaagacatcgacaaagctatgaaactgggtgctaaccacccgatgggtccgctggaactgggtgacttcatcggtctggacatctgcctggctatcatggacgttctgtactccgaaaccggtgactccaaataccgtccgcacaccctgctgaaaaaatacgttcgtgctggttggctgggtcgtaaatccggtaaaggtttctacgactactccaaacatcaccatcaccatcactaataa BBa_I72512_sequence 1 atggaactgaacaacgttatcctggaaaaagaaggtaaagttgctgttgttaccatcaaccgtccgaaagctctgaacgctctgaactccgacaccctgaaagaaatggactacgttatcggtgaaatcgaaaacgactccgaagttctggctgttatcctgaccggtgctggtgaaaaatccttcgttgctggtgctgacatctccgaaatgaaagaaatgaacaccatcgaaggtcgtaaattcggtatcctgggtaacaaagttttccgtcgtctggaactgctggaaaaaccggttatcgctgctgttaacggtttcgctctgggtggtggttgcgaaatcgctatgtcctgcgacatccgtatcgcttcctccaacgctcgtttcggtcagccggaagttggtctgggtatcaccccgggtttcggtggtacccagcgtctgtcccgtctggttggtatgggtatggctaaacagctgatcttcaccgctcagaacatcaaagctgacgaagctctgcgtatcggtctggttaacaaagttgttgaaccgtccgaactgatgaacaccgctaaagaaatcgctaacaaaatcgtttccaacgctccggttgctgttaaactgtccaaacaggctatcaaccgtggtatgcagtgcgacatcgacaccgctctggctttcgaatccgaagctttcggtgaatgcttctccaccgaagaccagaaagacgctatgaccgctttcatcgaaaaacgtaaaatcgaaggtttcaaaaaccgtcatcaccatcaccatcac BBa_S04145_sequence 1 aaagaggagaaatactagatgaaaaaagtttgcgttatcggtgctggtaccatgggttccggtatcgctcaggctttcgctgctaaaggtttcgaagttgttctgcgtgacatcaaagacgaatttgttgaccgtggtctggacttcatcaacaaaaacctgtccaaactggttaaaaaaggtaaaatcgaagaagctaccaaagttgaaatcctgacccgtatctccggtaccgttgacctgaacatggctgctgactgcgacctggttatcgaagctgctgttgaacgtatggacatcaaaaaacagatcttcgctgacctggacaacatctgcaaaccggaaaccatcctggcttccaacacctcctccctgtccatcaccgaagttgcttccgctaccaaacgtccggacaaagttatcggtatgcacttcttcaacccggctccggttatgaaactggttgaagttatccgtggtatcgctacctcccaggaaaccttcgacgctgttaaagaaacctccatcgctatcggtaaagacccggttgaagttgctgaagctccgggtttcgttgttaaccgtatcctgatcccgatgatcaacgaagctgttggtatcctggctgaaggtatcgcttccgttgaagacatcgacaaagctatgaaactgggtgctaaccacccgatgggtccgctggaactgggtgacttcatcggtctggacatctgcctggctatcatggacgttctgtactccgaaaccggtgactccaaataccgtccgcacaccctgctgaaaaaatacgttcgtgctggttggctgggtcgtaaatccggtaaaggtttctacgactactccaaacatcaccatcaccatcactaataatactagagaaagaggagaaatactagatggaactgaacaacgttatcctggaaaaagaaggtaaagttgctgttgttaccatcaaccgtccgaaagctctgaacgctctgaactccgacaccctgaaagaaatggactacgttatcggtgaaatcgaaaacgactccgaagttctggctgttatcctgaccggtgctggtgaaaaatccttcgttgctggtgctgacatctccgaaatgaaagaaatgaacaccatcgaaggtcgtaaattcggtatcctgggtaacaaagttttccgtcgtctggaactgctggaaaaaccggttatcgctgctgttaacggtttcgctctgggtggtggttgcgaaatcgctatgtcctgcgacatccgtatcgcttcctccaacgctcgtttcggtcagccggaagttggtctgggtatcaccccgggtttcggtggtacccagcgtctgtcccgtctggttggtatgggtatggctaaacagctgatcttcaccgctcagaacatcaaagctgacgaagctctgcgtatcggtctggttaacaaagttgttgaaccgtccgaactgatgaacaccgctaaagaaatcgctaacaaaatcgtttccaacgctccggttgctgttaaactgtccaaacaggctatcaaccgtggtatgcagtgcgacatcgacaccgctctggctttcgaatccgaagctttcggtgaatgcttctccaccgaagaccagaaagacgctatgaccgctttcatcgaaaaacgtaaaatcgaaggtttcaaaaaccgtcatcaccatcaccatcactactagagaaagaggagaaatactagatggacttcaacctgacccgtgaacaggaactggttcgtcagatggttcgtgagttcgctgaaaacgaagttaaaccgatcgctgctgaaatcgacgaaaccgaacgtttcccgatggaaaacgttaaaaaaatgggtcagtacggtatgatgggtatcccgttctccaaagaatacggtggtgctggtggtgacgttctgtcctacatcatcgctgttgaagaactgtccaaagtttgcggtaccaccggtgttatcctgtccgctcacacctccctgtgcgcttccctgatcaacgaacacggtaccgaagaacagaaacagaaatacctggttccgctggctaaaggtgaaaaaatcggtgcttacggtctgaccgaaccgaacgctggtaccgactccggtgctcagcagaccgttgctgttctggaaggtgaccactacgttatcaacggttccaaaatcttcatcaccaacggtggtgttgctgacaccttcgttatcttcgctatgaccgaccgtaccaaaggtaccaaaggtatctccgctttcatcatcgaaaaaggtttcaaaggtttctccatcggtaaagttgaacagaaactgggtatccgtgcttcctccaccaccgaactggttttcgaagacatgatcgttccggttgaaaacatgatcggtaaagaaggtaaaggtttcccgatcgctatgaaaaccctggacggtggtcgtatcggtatcgctgctcaggctctgggtatcgctgaaggtgctttcaacgaagctcgtgcttacatgaaagaacgtaaacagttcggtcgttccctggacaaattccagggtctggcttggatgatggctgacatggacgttgctatcgaatccgctcgttacctggtttacaaagctgcttacctgaaacaggctggtctgccgtacaccgttgacgctgctcgtgctaaactgcacgctgctaacgttgctatggacgttaccaccaaagctgttcagctgttcggtggttacggttacaccaaagactacccggttgaacgtatgatgcgtgacgctaaaatcaccgaaatctacgaaggtacctccgaagttcagaaactggttatctccggtaaaatcttccgtcatcaccatcaccatcac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z