BBa_I725013 1 BBa_I725013 Butyryl CoA dehyrogenase 2007-06-17T11:00:00Z 2015-08-31T04:07:54Z C. acetobutylicum genom. CAC_2712 Butyryl coa dehyrogenase from C. acetobutylicum. The sequence has been selected because it is part of an operon that has successfully been cloned into E. coli. It catalyzes the third step in butanol biosythesis. It has a His-tag added to its C terminus. false false _106_ 0 1824 9 It's complicated false The sequence has been optimized for use in E. Coli and has had its restriction sites removed. It has had a c-terminal His tag added and its stop codon has been changed to TAATAA. false Erin Dul BBa_S04146 1 BBa_S04146 S04144:S04145 2008-10-28T12:00:00Z 2015-05-08T01:14:35Z true false _9_ 0 2745 9 Discontinued false false Tom Tran component1993863 1 BBa_I725015 component1993866 1 BBa_I725011 component1993871 1 BBa_B0034 component1993859 1 BBa_B0034 component1993860 1 BBa_I725014 component1993869 1 BBa_I72512 component1993862 1 BBa_B0034 component1993868 1 BBa_B0034 component1993872 1 BBa_I725013 component1993865 1 BBa_B0034 annotation1993859 1 BBa_B0034 range1993859 1 1 12 annotation1993862 1 BBa_B0034 range1993862 1 2625 2636 annotation1993869 1 BBa_I72512 range1993869 1 4753 5553 annotation1993872 1 BBa_I725013 range1993872 1 5580 6734 annotation1993868 1 BBa_B0034 range1993868 1 4735 4746 annotation1993860 1 BBa_I725014 range1993860 1 19 2616 annotation1993866 1 BBa_I725011 range1993866 1 3857 4726 annotation1993865 1 BBa_B0034 range1993865 1 3839 3850 annotation1993871 1 BBa_B0034 range1993871 1 5562 5573 annotation1993863 1 BBa_I725015 range1993863 1 2643 3830 BBa_I72512 1 BBa_I72512 Enoyl-coa hydratase 2007-06-17T11:00:00Z 2015-08-31T04:07:54Z C. acetobutylicum genome. CAC_2712 Enoly-coa hydratase from C. acetobutylicum. The sequence has been selected because it is part of an operon that has successfully been cloned into E. coli. It catalyzes the second step in butanol biosythesis. It has a His-tag added to its C terminus. false false _106_ 0 1824 9 It's complicated false The sequence has had its codons optimized for E. coli and has had its restriction sites removed. The sequence has had its stop codon changed to TAATAA. It has had a His tag added to its c terminus. true Erin Dul BBa_I725011 1 BBa_I725011 B-hydroxy butyryl coA dehydrogenase 2007-06-17T11:00:00Z 2015-08-31T04:07:54Z Genomic sequence of Chlostridium acetobutylicum. CAC_2708. B-hydroxybutyryl coa dehydrogenase from C. acetobutylicum. Catalyzes the first step in the conversion of aceto-acetylcoa into butanol. It is from an operon containing this gene as well as two other genes in the butanol production pathway. It has been successfully cloned into e. coli before and activity has been recovered. The sequence has C-terminal His-tag. false false _106_ 0 1824 9 It's complicated false The sequence has been optimized for E. Coli and has had its restriction sites removed. A His tag has been added to the C terminus. The stop codon has been changed to TAATAA. true Erin Dul BBa_I725014 1 BBa_I725014 Butyraldehyde dehydrogenase 2007-06-17T11:00:00Z 2015-08-31T04:07:54Z pSOL plamid of C. acetobutylicum. CAP_0035 This is the butyraldehyde dehydrogenase from C. acetobutylicum. It catalyzes the fourth step in the reaction of butanol biosythesis. Its is from the pSOL plasmid of C. acetobutylicum. It also has some alcohol dehydrogenase activity. false false _106_ 0 1824 9 It's complicated false The sequence has been optimized for use in E. Coli and has had its restriction sites removed. It has had a c-terminal His tag added and its stop codon has been changed to TAATAA. false Erin Dul BBa_I725015 1 BBa_I725015 Butanol dehydrogenase 2007-06-17T11:00:00Z 2015-08-31T04:07:54Z C. acetobutylicum genome CAC_3298 Butanol dehydrogenase from C. acetobutylicum. This catalyzes the fifth step of butanol biosythesis. It has been cloned into e. coli previously and activity has been recovered. false false _106_ 0 1824 9 It's complicated false This part has been optimized for synthesis in E. Coli and has had its restriction sites removed. It has had its GTG start codon mutated to ATG and its stop codon mutated to TAATAA. It has also had ad C terminal His tag added. false Erin Dul BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I725014_sequence 1 atgaaagttaccaaccagaaagaactgaaacagaaactgaacgaactgcgtgaagctcagaaaaaattcgctacctacacccaggaacaggttgacaaaatcttcaaacagtgcgctatcgctgctgctaaagaacgtatcaacctggctaaactggctgttgaagaaaccggtatcggtctggttgaagacaaaatcatcaaaaaccacttcgctgctgaatacatctacaacaaatacaaaaacgaaaaaacctgcggtatcatcgaccacgacgactccctgggtatcaccaaagttgctgaaccgatcggtatcgttgctgctatcgttccgaccaccaacccgacctccaccgctatcttcaaatccctgatctccctgaaaacccgtaacgctatcttcttctccccgcacccgcgtgctaaaaaatccaccatcgctgctgctaaactgatcctggacgctgctgttaaagctggtgctccgaaaaacatcatcggttggatcgacgaaccgtccatcgaactgtcccaggacctgatgtccgaagctgacatcatcctggctaccggtggtccgtccatggttaaagctgcttactcctccggtaaaccggctatcggtgttggtgctggtaacaccccggctatcatcgacgaatccgctgacatcgacatggctgtttcctccatcatcctgtccaaaacctacgacaacggtgttatctgcgcttccgaacagtccatcctggttatgaactccatctacgaaaaagttaaagaagagttcgttaaacgtggttcctacatcctgaaccagaacgaaatcgctaaaatcaaagaaaccatgttcaaaaacggtgctatcaacgctgacatcgttggtaaatccgcttacatcatcgctaaaatggctggtatcgaagttccgcagaccaccaaaatcctgatcggtgaagttcagtccgttgaaaaatccgaactgttctcccacgaaaaactgtccccggttctggctatgtacaaagttaaagacttcgacgaagctctgaaaaaagctcagcgtctgatcgaactgggtggttccggtcacacctcctccctgtacatcgactcccagaacaacaaagacaaagttaaagagttcggtctggctatgaaaacctcccgtaccttcatcaacatgccgtcctcccagggtgcttccggtgacctgtacaacttcgctatcgctccgtccttcaccctgggttgcggtacctggggtggtaactccgtttcccagaacgttgaaccgaaacacctgctgaacatcaaatccgttgctgaacgtcgtgaaaacatgctgtggttcaaagttccgcagaaaatctacttcaaatacggttgcctgcgtttcgctctgaaagaactgaaagacatgaacaaaaaacgtgctttcatcgttaccgacaaagacctgttcaaactgggttacgttaacaaaatcaccaaagttctggacgaaatcgacatcaaatactccatcttcaccgacatcaaatccgacccgaccatcgactccgttaaaaaaggtgctaaagaaatgctgaacttcgaaccggacaccatcatctccatcggtggtggttccccgatggacgctgctaaagttatgcacctgctgtacgaatacccggaagctgaaatcgaaaacctggctatcaacttcatggacatccgtaaacgtatctgcaacttcccgaaactgggtaccaaagctatctccgttgctatcccgaccaccgctggtaccggttccgaagctaccccgttcgctgttatcaccaacgacgaaaccggtatgaaatacccgctgacctcctacgaactgaccccgaacatggctatcatcgacaccgaactgatgctgaacatgccgcgtaaactgaccgctgctaccggtatcgacgctctggttcacgctatcgaagcttacgtttccgttatggctaccgactacaccgacgaactggctctgcgtgctatcaaaatgatcttcaaatacctgccgcgtgcttacaaaaacggtaccaacgacatcgaagctcgtgaaaaaatggctcacgcttccaacatcgctggtatggctttcgctaacgctttcctgggtgtttgccactccatggctcacaaactgggtgctatgcaccacgttccgcacggtatcgcttgcgctgttctgatcgaagaagttatcaaatacaacgctaccgactgcccgaccaaacagaccgctttcccgcagtacaaatccccgaacgctaaacgtaaatacgctgaaatcgctgaatacctgaacctgaaaggtacctccgacaccgaaaaagttaccgctctgatcgaagctatctccaaactgaaaatcgacctgtccatcccgcagaacatctccgctgctggtatcaacaaaaaagacttctacaacaccctggacaaaatgtccgaactggctttcgacgaccagtgcaccaccgctaacccgcgttacccgctgatctccgaactgaaagacatctacatcaaatccttccatcaccatcaccatcactaataa BBa_I725013_sequence 1 atggacttcaacctgacccgtgaacaggaactggttcgtcagatggttcgtgagttcgctgaaaacgaagttaaaccgatcgctgctgaaatcgacgaaaccgaacgtttcccgatggaaaacgttaaaaaaatgggtcagtacggtatgatgggtatcccgttctccaaagaatacggtggtgctggtggtgacgttctgtcctacatcatcgctgttgaagaactgtccaaagtttgcggtaccaccggtgttatcctgtccgctcacacctccctgtgcgcttccctgatcaacgaacacggtaccgaagaacagaaacagaaatacctggttccgctggctaaaggtgaaaaaatcggtgcttacggtctgaccgaaccgaacgctggtaccgactccggtgctcagcagaccgttgctgttctggaaggtgaccactacgttatcaacggttccaaaatcttcatcaccaacggtggtgttgctgacaccttcgttatcttcgctatgaccgaccgtaccaaaggtaccaaaggtatctccgctttcatcatcgaaaaaggtttcaaaggtttctccatcggtaaagttgaacagaaactgggtatccgtgcttcctccaccaccgaactggttttcgaagacatgatcgttccggttgaaaacatgatcggtaaagaaggtaaaggtttcccgatcgctatgaaaaccctggacggtggtcgtatcggtatcgctgctcaggctctgggtatcgctgaaggtgctttcaacgaagctcgtgcttacatgaaagaacgtaaacagttcggtcgttccctggacaaattccagggtctggcttggatgatggctgacatggacgttgctatcgaatccgctcgttacctggtttacaaagctgcttacctgaaacaggctggtctgccgtacaccgttgacgctgctcgtgctaaactgcacgctgctaacgttgctatggacgttaccaccaaagctgttcagctgttcggtggttacggttacaccaaagactacccggttgaacgtatgatgcgtgacgctaaaatcaccgaaatctacgaaggtacctccgaagttcagaaactggttatctccggtaaaatcttccgtcatcaccatcaccatcac BBa_I725015_sequence 1 atggttgacttcgaatactccatcccgacccgtatcttcttcggtaaagacaaaatcaacgttctgggtcgtgaactgaaaaaatacggttccaaagttctgatcgtttacggtggtggttccatcaaacgtaacggtatctacgacaaagctgtttccatcctggaaaaaaactccatcaaattctacgaactggctggtgttgaaccgaacccgcgtgttaccaccgttgaaaaaggtgttaaaatctgccgtgaaaacggtgttgaagttgttctggctatcggtggtggttccgctatcgactgcgctaaagttatcgctgctgcttgcgaatacgacggtaacccgtgggacatcgttctggacggttccaaaatcaaacgtgttctgccgatcgcttccatcctgaccatcgctgctaccggttccgaaatggacacctgggctgttatcaacaacatggacaccaacgaaaaactgatcgctgctcacccggacatggctccgaaattctccatcctggacccgacctacacctacaccgttccgaccaaccagaccgctgctggtaccgctgacatcatgtcccacatcttcgaagtttacttctccaacaccaaaaccgcttacctgcaagaccgtatggctgaagctctgctgcgtacctgcatcaaatacggtggtatcgctctggaaaaaccggacgactacgaagctcgtgctaacctgatgtgggcttcctccctggctatcaacggtctgctgacctacggtaaagacaccaactggtccgttcacctgatggaacacgaactgtccgcttactacgacatcacccacggtgttggtctggctatcctgaccccgaactggatggaatacatcctgaacaacgacaccgtttacaaattcgttgaatacggtgttaacgtttggggtatcgacaaagaaaaaaaccactacgacatcgctcaccaggctatccagaaaacccgtgactacttcgttaacgttctgggtctgccgtcccgtctgcgtgacgttggtatcgaagaagaaaaactggacatcatggctaaagaatccgttaaactgaccggtggtaccatcggtaacctgcgtccggttaacgcttccgaagttctgcaaatcttcaaaaaatccgttcatcaccatcaccatcac BBa_B0034_sequence 1 aaagaggagaaa BBa_I725011_sequence 1 atgaaaaaagtttgcgttatcggtgctggtaccatgggttccggtatcgctcaggctttcgctgctaaaggtttcgaagttgttctgcgtgacatcaaagacgaatttgttgaccgtggtctggacttcatcaacaaaaacctgtccaaactggttaaaaaaggtaaaatcgaagaagctaccaaagttgaaatcctgacccgtatctccggtaccgttgacctgaacatggctgctgactgcgacctggttatcgaagctgctgttgaacgtatggacatcaaaaaacagatcttcgctgacctggacaacatctgcaaaccggaaaccatcctggcttccaacacctcctccctgtccatcaccgaagttgcttccgctaccaaacgtccggacaaagttatcggtatgcacttcttcaacccggctccggttatgaaactggttgaagttatccgtggtatcgctacctcccaggaaaccttcgacgctgttaaagaaacctccatcgctatcggtaaagacccggttgaagttgctgaagctccgggtttcgttgttaaccgtatcctgatcccgatgatcaacgaagctgttggtatcctggctgaaggtatcgcttccgttgaagacatcgacaaagctatgaaactgggtgctaaccacccgatgggtccgctggaactgggtgacttcatcggtctggacatctgcctggctatcatggacgttctgtactccgaaaccggtgactccaaataccgtccgcacaccctgctgaaaaaatacgttcgtgctggttggctgggtcgtaaatccggtaaaggtttctacgactactccaaacatcaccatcaccatcactaataa BBa_S04146_sequence 1 aaagaggagaaatactagatgaaagttaccaaccagaaagaactgaaacagaaactgaacgaactgcgtgaagctcagaaaaaattcgctacctacacccaggaacaggttgacaaaatcttcaaacagtgcgctatcgctgctgctaaagaacgtatcaacctggctaaactggctgttgaagaaaccggtatcggtctggttgaagacaaaatcatcaaaaaccacttcgctgctgaatacatctacaacaaatacaaaaacgaaaaaacctgcggtatcatcgaccacgacgactccctgggtatcaccaaagttgctgaaccgatcggtatcgttgctgctatcgttccgaccaccaacccgacctccaccgctatcttcaaatccctgatctccctgaaaacccgtaacgctatcttcttctccccgcacccgcgtgctaaaaaatccaccatcgctgctgctaaactgatcctggacgctgctgttaaagctggtgctccgaaaaacatcatcggttggatcgacgaaccgtccatcgaactgtcccaggacctgatgtccgaagctgacatcatcctggctaccggtggtccgtccatggttaaagctgcttactcctccggtaaaccggctatcggtgttggtgctggtaacaccccggctatcatcgacgaatccgctgacatcgacatggctgtttcctccatcatcctgtccaaaacctacgacaacggtgttatctgcgcttccgaacagtccatcctggttatgaactccatctacgaaaaagttaaagaagagttcgttaaacgtggttcctacatcctgaaccagaacgaaatcgctaaaatcaaagaaaccatgttcaaaaacggtgctatcaacgctgacatcgttggtaaatccgcttacatcatcgctaaaatggctggtatcgaagttccgcagaccaccaaaatcctgatcggtgaagttcagtccgttgaaaaatccgaactgttctcccacgaaaaactgtccccggttctggctatgtacaaagttaaagacttcgacgaagctctgaaaaaagctcagcgtctgatcgaactgggtggttccggtcacacctcctccctgtacatcgactcccagaacaacaaagacaaagttaaagagttcggtctggctatgaaaacctcccgtaccttcatcaacatgccgtcctcccagggtgcttccggtgacctgtacaacttcgctatcgctccgtccttcaccctgggttgcggtacctggggtggtaactccgtttcccagaacgttgaaccgaaacacctgctgaacatcaaatccgttgctgaacgtcgtgaaaacatgctgtggttcaaagttccgcagaaaatctacttcaaatacggttgcctgcgtttcgctctgaaagaactgaaagacatgaacaaaaaacgtgctttcatcgttaccgacaaagacctgttcaaactgggttacgttaacaaaatcaccaaagttctggacgaaatcgacatcaaatactccatcttcaccgacatcaaatccgacccgaccatcgactccgttaaaaaaggtgctaaagaaatgctgaacttcgaaccggacaccatcatctccatcggtggtggttccccgatggacgctgctaaagttatgcacctgctgtacgaatacccggaagctgaaatcgaaaacctggctatcaacttcatggacatccgtaaacgtatctgcaacttcccgaaactgggtaccaaagctatctccgttgctatcccgaccaccgctggtaccggttccgaagctaccccgttcgctgttatcaccaacgacgaaaccggtatgaaatacccgctgacctcctacgaactgaccccgaacatggctatcatcgacaccgaactgatgctgaacatgccgcgtaaactgaccgctgctaccggtatcgacgctctggttcacgctatcgaagcttacgtttccgttatggctaccgactacaccgacgaactggctctgcgtgctatcaaaatgatcttcaaatacctgccgcgtgcttacaaaaacggtaccaacgacatcgaagctcgtgaaaaaatggctcacgcttccaacatcgctggtatggctttcgctaacgctttcctgggtgtttgccactccatggctcacaaactgggtgctatgcaccacgttccgcacggtatcgcttgcgctgttctgatcgaagaagttatcaaatacaacgctaccgactgcccgaccaaacagaccgctttcccgcagtacaaatccccgaacgctaaacgtaaatacgctgaaatcgctgaatacctgaacctgaaaggtacctccgacaccgaaaaagttaccgctctgatcgaagctatctccaaactgaaaatcgacctgtccatcccgcagaacatctccgctgctggtatcaacaaaaaagacttctacaacaccctggacaaaatgtccgaactggctttcgacgaccagtgcaccaccgctaacccgcgttacccgctgatctccgaactgaaagacatctacatcaaatccttccatcaccatcaccatcactaataatactagagaaagaggagaaatactagatggttgacttcgaatactccatcccgacccgtatcttcttcggtaaagacaaaatcaacgttctgggtcgtgaactgaaaaaatacggttccaaagttctgatcgtttacggtggtggttccatcaaacgtaacggtatctacgacaaagctgtttccatcctggaaaaaaactccatcaaattctacgaactggctggtgttgaaccgaacccgcgtgttaccaccgttgaaaaaggtgttaaaatctgccgtgaaaacggtgttgaagttgttctggctatcggtggtggttccgctatcgactgcgctaaagttatcgctgctgcttgcgaatacgacggtaacccgtgggacatcgttctggacggttccaaaatcaaacgtgttctgccgatcgcttccatcctgaccatcgctgctaccggttccgaaatggacacctgggctgttatcaacaacatggacaccaacgaaaaactgatcgctgctcacccggacatggctccgaaattctccatcctggacccgacctacacctacaccgttccgaccaaccagaccgctgctggtaccgctgacatcatgtcccacatcttcgaagtttacttctccaacaccaaaaccgcttacctgcaagaccgtatggctgaagctctgctgcgtacctgcatcaaatacggtggtatcgctctggaaaaaccggacgactacgaagctcgtgctaacctgatgtgggcttcctccctggctatcaacggtctgctgacctacggtaaagacaccaactggtccgttcacctgatggaacacgaactgtccgcttactacgacatcacccacggtgttggtctggctatcctgaccccgaactggatggaatacatcctgaacaacgacaccgtttacaaattcgttgaatacggtgttaacgtttggggtatcgacaaagaaaaaaaccactacgacatcgctcaccaggctatccagaaaacccgtgactacttcgttaacgttctgggtctgccgtcccgtctgcgtgacgttggtatcgaagaagaaaaactggacatcatggctaaagaatccgttaaactgaccggtggtaccatcggtaacctgcgtccggttaacgcttccgaagttctgcaaatcttcaaaaaatccgttcatcaccatcaccatcactactagagaaagaggagaaatactagatgaaaaaagtttgcgttatcggtgctggtaccatgggttccggtatcgctcaggctttcgctgctaaaggtttcgaagttgttctgcgtgacatcaaagacgaatttgttgaccgtggtctggacttcatcaacaaaaacctgtccaaactggttaaaaaaggtaaaatcgaagaagctaccaaagttgaaatcctgacccgtatctccggtaccgttgacctgaacatggctgctgactgcgacctggttatcgaagctgctgttgaacgtatggacatcaaaaaacagatcttcgctgacctggacaacatctgcaaaccggaaaccatcctggcttccaacacctcctccctgtccatcaccgaagttgcttccgctaccaaacgtccggacaaagttatcggtatgcacttcttcaacccggctccggttatgaaactggttgaagttatccgtggtatcgctacctcccaggaaaccttcgacgctgttaaagaaacctccatcgctatcggtaaagacccggttgaagttgctgaagctccgggtttcgttgttaaccgtatcctgatcccgatgatcaacgaagctgttggtatcctggctgaaggtatcgcttccgttgaagacatcgacaaagctatgaaactgggtgctaaccacccgatgggtccgctggaactgggtgacttcatcggtctggacatctgcctggctatcatggacgttctgtactccgaaaccggtgactccaaataccgtccgcacaccctgctgaaaaaatacgttcgtgctggttggctgggtcgtaaatccggtaaaggtttctacgactactccaaacatcaccatcaccatcactaataatactagagaaagaggagaaatactagatggaactgaacaacgttatcctggaaaaagaaggtaaagttgctgttgttaccatcaaccgtccgaaagctctgaacgctctgaactccgacaccctgaaagaaatggactacgttatcggtgaaatcgaaaacgactccgaagttctggctgttatcctgaccggtgctggtgaaaaatccttcgttgctggtgctgacatctccgaaatgaaagaaatgaacaccatcgaaggtcgtaaattcggtatcctgggtaacaaagttttccgtcgtctggaactgctggaaaaaccggttatcgctgctgttaacggtttcgctctgggtggtggttgcgaaatcgctatgtcctgcgacatccgtatcgcttcctccaacgctcgtttcggtcagccggaagttggtctgggtatcaccccgggtttcggtggtacccagcgtctgtcccgtctggttggtatgggtatggctaaacagctgatcttcaccgctcagaacatcaaagctgacgaagctctgcgtatcggtctggttaacaaagttgttgaaccgtccgaactgatgaacaccgctaaagaaatcgctaacaaaatcgtttccaacgctccggttgctgttaaactgtccaaacaggctatcaaccgtggtatgcagtgcgacatcgacaccgctctggctttcgaatccgaagctttcggtgaatgcttctccaccgaagaccagaaagacgctatgaccgctttcatcgaaaaacgtaaaatcgaaggtttcaaaaaccgtcatcaccatcaccatcactactagagaaagaggagaaatactagatggacttcaacctgacccgtgaacaggaactggttcgtcagatggttcgtgagttcgctgaaaacgaagttaaaccgatcgctgctgaaatcgacgaaaccgaacgtttcccgatggaaaacgttaaaaaaatgggtcagtacggtatgatgggtatcccgttctccaaagaatacggtggtgctggtggtgacgttctgtcctacatcatcgctgttgaagaactgtccaaagtttgcggtaccaccggtgttatcctgtccgctcacacctccctgtgcgcttccctgatcaacgaacacggtaccgaagaacagaaacagaaatacctggttccgctggctaaaggtgaaaaaatcggtgcttacggtctgaccgaaccgaacgctggtaccgactccggtgctcagcagaccgttgctgttctggaaggtgaccactacgttatcaacggttccaaaatcttcatcaccaacggtggtgttgctgacaccttcgttatcttcgctatgaccgaccgtaccaaaggtaccaaaggtatctccgctttcatcatcgaaaaaggtttcaaaggtttctccatcggtaaagttgaacagaaactgggtatccgtgcttcctccaccaccgaactggttttcgaagacatgatcgttccggttgaaaacatgatcggtaaagaaggtaaaggtttcccgatcgctatgaaaaccctggacggtggtcgtatcggtatcgctgctcaggctctgggtatcgctgaaggtgctttcaacgaagctcgtgcttacatgaaagaacgtaaacagttcggtcgttccctggacaaattccagggtctggcttggatgatggctgacatggacgttgctatcgaatccgctcgttacctggtttacaaagctgcttacctgaaacaggctggtctgccgtacaccgttgacgctgctcgtgctaaactgcacgctgctaacgttgctatggacgttaccaccaaagctgttcagctgttcggtggttacggttacaccaaagactacccggttgaacgtatgatgcgtgacgctaaaatcaccgaaatctacgaaggtacctccgaagttcagaaactggttatctccggtaaaatcttccgtcatcaccatcaccatcac BBa_I72512_sequence 1 atggaactgaacaacgttatcctggaaaaagaaggtaaagttgctgttgttaccatcaaccgtccgaaagctctgaacgctctgaactccgacaccctgaaagaaatggactacgttatcggtgaaatcgaaaacgactccgaagttctggctgttatcctgaccggtgctggtgaaaaatccttcgttgctggtgctgacatctccgaaatgaaagaaatgaacaccatcgaaggtcgtaaattcggtatcctgggtaacaaagttttccgtcgtctggaactgctggaaaaaccggttatcgctgctgttaacggtttcgctctgggtggtggttgcgaaatcgctatgtcctgcgacatccgtatcgcttcctccaacgctcgtttcggtcagccggaagttggtctgggtatcaccccgggtttcggtggtacccagcgtctgtcccgtctggttggtatgggtatggctaaacagctgatcttcaccgctcagaacatcaaagctgacgaagctctgcgtatcggtctggttaacaaagttgttgaaccgtccgaactgatgaacaccgctaaagaaatcgctaacaaaatcgtttccaacgctccggttgctgttaaactgtccaaacaggctatcaaccgtggtatgcagtgcgacatcgacaccgctctggctttcgaatccgaagctttcggtgaatgcttctccaccgaagaccagaaagacgctatgaccgctttcatcgaaaaacgtaaaatcgaaggtttcaaaaaccgtcatcaccatcaccatcac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z