LOCUS BBa_S04147 6734 bp DNA linear 26-Nov-2024 DEFINITION The Partial Butanol Operon (5 inserts from I725021 to I725025) ACCESSION BBa_S04147 VERSION BBa_S04147.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..888 /label=BBa_I725011 misc_feature 901..904 /label=conserved RBS 897..908 /label=BBa_B0034 CDS 915..1715 /label=BBa_I72512 misc_feature 1728..1731 /label=conserved RBS 1724..1735 /label=BBa_B0034 CDS 1742..2896 /label=BBa_I725013 misc_feature 2909..2912 /label=conserved RBS 2905..2916 /label=BBa_B0034 CDS 2923..5520 /label=BBa_I725014 misc_feature 5533..5536 /label=conserved RBS 5529..5540 /label=BBa_B0034 CDS 5547..6734 /label=BBa_I725015 ORIGIN 1 aaagaggaga aatactagat gaaaaaagtt tgcgttatcg gtgctggtac catgggttcc 61 ggtatcgctc aggctttcgc tgctaaaggt ttcgaagttg ttctgcgtga catcaaagac 121 gaatttgttg accgtggtct ggacttcatc aacaaaaacc tgtccaaact ggttaaaaaa 181 ggtaaaatcg aagaagctac caaagttgaa atcctgaccc gtatctccgg taccgttgac 241 ctgaacatgg ctgctgactg cgacctggtt atcgaagctg ctgttgaacg tatggacatc 301 aaaaaacaga tcttcgctga cctggacaac atctgcaaac cggaaaccat cctggcttcc 361 aacacctcct ccctgtccat caccgaagtt gcttccgcta ccaaacgtcc ggacaaagtt 421 atcggtatgc acttcttcaa cccggctccg gttatgaaac tggttgaagt tatccgtggt 481 atcgctacct cccaggaaac cttcgacgct gttaaagaaa cctccatcgc tatcggtaaa 541 gacccggttg aagttgctga agctccgggt ttcgttgtta accgtatcct gatcccgatg 601 atcaacgaag ctgttggtat cctggctgaa ggtatcgctt ccgttgaaga catcgacaaa 661 gctatgaaac tgggtgctaa ccacccgatg ggtccgctgg aactgggtga cttcatcggt 721 ctggacatct gcctggctat catggacgtt ctgtactccg aaaccggtga ctccaaatac 781 cgtccgcaca ccctgctgaa aaaatacgtt cgtgctggtt ggctgggtcg taaatccggt 841 aaaggtttct acgactactc caaacatcac catcaccatc actaataata ctagagaaag 901 aggagaaata ctagatggaa ctgaacaacg ttatcctgga aaaagaaggt aaagttgctg 961 ttgttaccat caaccgtccg aaagctctga acgctctgaa ctccgacacc ctgaaagaaa 1021 tggactacgt tatcggtgaa atcgaaaacg actccgaagt tctggctgtt atcctgaccg 1081 gtgctggtga aaaatccttc gttgctggtg ctgacatctc cgaaatgaaa gaaatgaaca 1141 ccatcgaagg tcgtaaattc ggtatcctgg gtaacaaagt tttccgtcgt ctggaactgc 1201 tggaaaaacc ggttatcgct gctgttaacg gtttcgctct gggtggtggt tgcgaaatcg 1261 ctatgtcctg cgacatccgt atcgcttcct ccaacgctcg tttcggtcag ccggaagttg 1321 gtctgggtat caccccgggt ttcggtggta cccagcgtct gtcccgtctg gttggtatgg 1381 gtatggctaa acagctgatc ttcaccgctc agaacatcaa agctgacgaa gctctgcgta 1441 tcggtctggt taacaaagtt gttgaaccgt ccgaactgat gaacaccgct aaagaaatcg 1501 ctaacaaaat cgtttccaac gctccggttg ctgttaaact gtccaaacag gctatcaacc 1561 gtggtatgca gtgcgacatc gacaccgctc tggctttcga atccgaagct ttcggtgaat 1621 gcttctccac cgaagaccag aaagacgcta tgaccgcttt catcgaaaaa cgtaaaatcg 1681 aaggtttcaa aaaccgtcat caccatcacc atcactacta gagaaagagg agaaatacta 1741 gatggacttc aacctgaccc gtgaacagga actggttcgt cagatggttc gtgagttcgc 1801 tgaaaacgaa gttaaaccga tcgctgctga aatcgacgaa accgaacgtt tcccgatgga 1861 aaacgttaaa aaaatgggtc agtacggtat gatgggtatc ccgttctcca aagaatacgg 1921 tggtgctggt ggtgacgttc tgtcctacat catcgctgtt gaagaactgt ccaaagtttg 1981 cggtaccacc ggtgttatcc tgtccgctca cacctccctg tgcgcttccc tgatcaacga 2041 acacggtacc gaagaacaga aacagaaata cctggttccg ctggctaaag gtgaaaaaat 2101 cggtgcttac ggtctgaccg aaccgaacgc tggtaccgac tccggtgctc agcagaccgt 2161 tgctgttctg gaaggtgacc actacgttat caacggttcc aaaatcttca tcaccaacgg 2221 tggtgttgct gacaccttcg ttatcttcgc tatgaccgac cgtaccaaag gtaccaaagg 2281 tatctccgct ttcatcatcg aaaaaggttt caaaggtttc tccatcggta aagttgaaca 2341 gaaactgggt atccgtgctt cctccaccac cgaactggtt ttcgaagaca tgatcgttcc 2401 ggttgaaaac atgatcggta aagaaggtaa aggtttcccg atcgctatga aaaccctgga 2461 cggtggtcgt atcggtatcg ctgctcaggc tctgggtatc gctgaaggtg ctttcaacga 2521 agctcgtgct tacatgaaag aacgtaaaca gttcggtcgt tccctggaca aattccaggg 2581 tctggcttgg atgatggctg acatggacgt tgctatcgaa tccgctcgtt acctggttta 2641 caaagctgct tacctgaaac aggctggtct gccgtacacc gttgacgctg ctcgtgctaa 2701 actgcacgct gctaacgttg ctatggacgt taccaccaaa gctgttcagc tgttcggtgg 2761 ttacggttac accaaagact acccggttga acgtatgatg cgtgacgcta aaatcaccga 2821 aatctacgaa ggtacctccg aagttcagaa actggttatc tccggtaaaa tcttccgtca 2881 tcaccatcac catcactact agagaaagag gagaaatact agatgaaagt taccaaccag 2941 aaagaactga aacagaaact gaacgaactg cgtgaagctc agaaaaaatt cgctacctac 3001 acccaggaac aggttgacaa aatcttcaaa cagtgcgcta tcgctgctgc taaagaacgt 3061 atcaacctgg ctaaactggc tgttgaagaa accggtatcg gtctggttga agacaaaatc 3121 atcaaaaacc acttcgctgc tgaatacatc tacaacaaat acaaaaacga aaaaacctgc 3181 ggtatcatcg accacgacga ctccctgggt atcaccaaag ttgctgaacc gatcggtatc 3241 gttgctgcta tcgttccgac caccaacccg acctccaccg ctatcttcaa atccctgatc 3301 tccctgaaaa cccgtaacgc tatcttcttc tccccgcacc cgcgtgctaa aaaatccacc 3361 atcgctgctg ctaaactgat cctggacgct gctgttaaag ctggtgctcc gaaaaacatc 3421 atcggttgga tcgacgaacc gtccatcgaa ctgtcccagg acctgatgtc cgaagctgac 3481 atcatcctgg ctaccggtgg tccgtccatg gttaaagctg cttactcctc cggtaaaccg 3541 gctatcggtg ttggtgctgg taacaccccg gctatcatcg acgaatccgc tgacatcgac 3601 atggctgttt cctccatcat cctgtccaaa acctacgaca acggtgttat ctgcgcttcc 3661 gaacagtcca tcctggttat gaactccatc tacgaaaaag ttaaagaaga gttcgttaaa 3721 cgtggttcct acatcctgaa ccagaacgaa atcgctaaaa tcaaagaaac catgttcaaa 3781 aacggtgcta tcaacgctga catcgttggt aaatccgctt acatcatcgc taaaatggct 3841 ggtatcgaag ttccgcagac caccaaaatc ctgatcggtg aagttcagtc cgttgaaaaa 3901 tccgaactgt tctcccacga aaaactgtcc ccggttctgg ctatgtacaa agttaaagac 3961 ttcgacgaag ctctgaaaaa agctcagcgt ctgatcgaac tgggtggttc cggtcacacc 4021 tcctccctgt acatcgactc ccagaacaac aaagacaaag ttaaagagtt cggtctggct 4081 atgaaaacct cccgtacctt catcaacatg ccgtcctccc agggtgcttc cggtgacctg 4141 tacaacttcg ctatcgctcc gtccttcacc ctgggttgcg gtacctgggg tggtaactcc 4201 gtttcccaga acgttgaacc gaaacacctg ctgaacatca aatccgttgc tgaacgtcgt 4261 gaaaacatgc tgtggttcaa agttccgcag aaaatctact tcaaatacgg ttgcctgcgt 4321 ttcgctctga aagaactgaa agacatgaac aaaaaacgtg ctttcatcgt taccgacaaa 4381 gacctgttca aactgggtta cgttaacaaa atcaccaaag ttctggacga aatcgacatc 4441 aaatactcca tcttcaccga catcaaatcc gacccgacca tcgactccgt taaaaaaggt 4501 gctaaagaaa tgctgaactt cgaaccggac accatcatct ccatcggtgg tggttccccg 4561 atggacgctg ctaaagttat gcacctgctg tacgaatacc cggaagctga aatcgaaaac 4621 ctggctatca acttcatgga catccgtaaa cgtatctgca acttcccgaa actgggtacc 4681 aaagctatct ccgttgctat cccgaccacc gctggtaccg gttccgaagc taccccgttc 4741 gctgttatca ccaacgacga aaccggtatg aaatacccgc tgacctccta cgaactgacc 4801 ccgaacatgg ctatcatcga caccgaactg atgctgaaca tgccgcgtaa actgaccgct 4861 gctaccggta tcgacgctct ggttcacgct atcgaagctt acgtttccgt tatggctacc 4921 gactacaccg acgaactggc tctgcgtgct atcaaaatga tcttcaaata cctgccgcgt 4981 gcttacaaaa acggtaccaa cgacatcgaa gctcgtgaaa aaatggctca cgcttccaac 5041 atcgctggta tggctttcgc taacgctttc ctgggtgttt gccactccat ggctcacaaa 5101 ctgggtgcta tgcaccacgt tccgcacggt atcgcttgcg ctgttctgat cgaagaagtt 5161 atcaaataca acgctaccga ctgcccgacc aaacagaccg ctttcccgca gtacaaatcc 5221 ccgaacgcta aacgtaaata cgctgaaatc gctgaatacc tgaacctgaa aggtacctcc 5281 gacaccgaaa aagttaccgc tctgatcgaa gctatctcca aactgaaaat cgacctgtcc 5341 atcccgcaga acatctccgc tgctggtatc aacaaaaaag acttctacaa caccctggac 5401 aaaatgtccg aactggcttt cgacgaccag tgcaccaccg ctaacccgcg ttacccgctg 5461 atctccgaac tgaaagacat ctacatcaaa tccttccatc accatcacca tcactaataa 5521 tactagagaa agaggagaaa tactagatgg ttgacttcga atactccatc ccgacccgta 5581 tcttcttcgg taaagacaaa atcaacgttc tgggtcgtga actgaaaaaa tacggttcca 5641 aagttctgat cgtttacggt ggtggttcca tcaaacgtaa cggtatctac gacaaagctg 5701 tttccatcct ggaaaaaaac tccatcaaat tctacgaact ggctggtgtt gaaccgaacc 5761 cgcgtgttac caccgttgaa aaaggtgtta aaatctgccg tgaaaacggt gttgaagttg 5821 ttctggctat cggtggtggt tccgctatcg actgcgctaa agttatcgct gctgcttgcg 5881 aatacgacgg taacccgtgg gacatcgttc tggacggttc caaaatcaaa cgtgttctgc 5941 cgatcgcttc catcctgacc atcgctgcta ccggttccga aatggacacc tgggctgtta 6001 tcaacaacat ggacaccaac gaaaaactga tcgctgctca cccggacatg gctccgaaat 6061 tctccatcct ggacccgacc tacacctaca ccgttccgac caaccagacc gctgctggta 6121 ccgctgacat catgtcccac atcttcgaag tttacttctc caacaccaaa accgcttacc 6181 tgcaagaccg tatggctgaa gctctgctgc gtacctgcat caaatacggt ggtatcgctc 6241 tggaaaaacc ggacgactac gaagctcgtg ctaacctgat gtgggcttcc tccctggcta 6301 tcaacggtct gctgacctac ggtaaagaca ccaactggtc cgttcacctg atggaacacg 6361 aactgtccgc ttactacgac atcacccacg gtgttggtct ggctatcctg accccgaact 6421 ggatggaata catcctgaac aacgacaccg tttacaaatt cgttgaatac ggtgttaacg 6481 tttggggtat cgacaaagaa aaaaaccact acgacatcgc tcaccaggct atccagaaaa 6541 cccgtgacta cttcgttaac gttctgggtc tgccgtcccg tctgcgtgac gttggtatcg 6601 aagaagaaaa actggacatc atggctaaag aatccgttaa actgaccggt ggtaccatcg 6661 gtaacctgcg tccggttaac gcttccgaag ttctgcaaat cttcaaaaaa tccgttcatc 6721 accatcacca tcac //