LOCUS       BBa_S04151              1077 bp    DNA     linear       14-Nov-2024
DEFINITION  S04149:S04150
ACCESSION   BBa_S04151
VERSION     BBa_S04151.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             83..940
                     /label=BBa_J36801
     stem_loop       960..1003
                     /label=stem_loop
     misc_feature    949..1028
                     /label=BBa_B0010
     terminator      949..1028
                     /label=BBa_B0010
     stem_loop       1044..1063
                     /label=T7 TE
     misc_feature    1070..1070
                     /label=stop
     polyA_site      1064..1077
                     /label=polya
     misc_feature    1037..1077
                     /label=BBa_B0012
     terminator      1037..1077
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag aggtgctctc gcaaattgca atctgcattt gggtggaatc
      121 gacggcaatt ttgcaggatt gccagcgggc gctgtcggcc gatcgctatc aactccaagt
      181 ctgtgagtct ggcgaaatgc tcttggagta tgcccaaacc catcgtgacc aaatcgactg
      241 cctgatttta gtggcagcca atcccagctt cagggcagtt gttcagcagc tctgctttga
      301 gggagtggtg gtaccagcga ttgtcgtagg cgatcgcgac agtgaggatc ccgatgaacc
      361 agccaaagaa cagctctatc acagcgctga actgcacctc ggtatccatc agctcgagca
      421 attgccctac caagttgatg ctgcactggc tgaatttctg cgcttagccc cggtcgagac
      481 catggccgac cacatcatgc tgatgggggc caaccacgat cccgagctat cgagccagca
      541 gcgggacctc gctcagcgac tacaagagcg cctaggctat ctcggggtct actacaagcg
      601 tgatcccgat cgctttctgc gcaacctacc cgcctacgaa agccaaaagc tgcaccaagc
      661 gatgcagact agctatcgtg aaatcgtttt gagctatttt tcgccgaata gcaacctcaa
      721 ccagagcatt gacaacttcg tcaacatggc tttctttgcc gatgttccag tcaccaaagt
      781 ggtagaaatt cacatggagc tgatggacga gtttgccaag aagctccgcg tagagggacg
      841 ttcagaggac attttgctgg attatcggct gactttaatt gatgtaattg cacatctttg
      901 tgagatgtat cgacggtcta tcccacgaga aacctgatga tactagagcc aggcatcaaa
      961 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1021 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//