BBa_S04160
1
BBa_S04160
K105001:K105012
2008-10-29T12:00:00Z
2015-05-08T01:14:36Z
true
false
_9_
0
3313
9
Discontinued
false
false
Katja Karstens
component1997053
1
BBa_K105012
component1997052
1
BBa_K105001
annotation1997053
1
BBa_K105012
range1997053
1
285
314
annotation1997052
1
BBa_K105001
range1997052
1
1
276
BBa_K105001
1
BBa_K105001
VP16 - eucaryotic activation domain
2008-10-11T11:00:00Z
2015-05-08T01:08:52Z
We have constructed our BioBrick from the plasmid xxx, which was available in our hosting lab.
Originally VP16 is a protein from the herpes simplex virus. It activates the transcription of the late genes of this virus by the RNA-polymerase II of the eucaryotic host.
In artificial systems VP16 is used in fusion with a DNA-binding domain. This DNA-binding domain is specific for a bait sequence. So the selective expression of genes with a promoter containing this bait sequence can be achieved.
false
false
_253_
0
3313
9
It's complicated
true
To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. <br\n>
For more information about this issus, see Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." DSpace http://hdl.handle.net/1721.1/32535 (2006).
true
Manuel Gersbacher
BBa_K105012
1
BBa_K105012
10 aa flexible protein domain linker
2008-10-18T11:00:00Z
2015-05-08T01:08:52Z
Oligos with a yeast optimized coding for the peptide GENLYFQSGG have been ordered
<br\n>
The amino acide sequence corresponds to the interdomaine linker of xxx.
''paper''
This is a 10 amino acides long linker peptide which can be used to join protein domains together in a flexible way. So fusion proteins with variable DNA-binding and activation or repression domains might be assembled.
<br\n>
false
true
_253_
0
3313
9
In stock
true
To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n>
For more information about this issus, see:<br\n>
Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n>
DSpace http://hdl.handle.net/1721.1/32535 (2006).
true
Manuel Gersbacher, Katja Karstens
BBa_K105001_sequence
1
gctagcgccgccaccatgggccctaaaaagaagcgtaaagtcgcccccccgaccgatgtcagcctgggggacgagctccacttagacggcgaggacgtggcgatggcgcatgccgacgcgctagacgatttcgatctggacatgttgggggacggggattccccggggccgggatttaccccccacgactccgccccctacggcgctctggatatggccgacttcgagtttgagcagatgtttaccgatgcccttggaattgacgagtacggtggg
BBa_S04160_sequence
1
gctagcgccgccaccatgggccctaaaaagaagcgtaaagtcgcccccccgaccgatgtcagcctgggggacgagctccacttagacggcgaggacgtggcgatggcgcatgccgacgcgctagacgatttcgatctggacatgttgggggacggggattccccggggccgggatttaccccccacgactccgccccctacggcgctctggatatggccgacttcgagtttgagcagatgtttaccgatgcccttggaattgacgagtacggtgggtactagagggtgaaaatttgtattttcaatctggtggt
BBa_K105012_sequence
1
ggtgaaaatttgtattttcaatctggtggt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z