LOCUS       BBa_S04166              1077 bp    DNA     linear       23-Dec-2024
DEFINITION  B0015:S04021
ACCESSION   BBa_S04166
VERSION     BBa_S04166.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     promoter        154..159
                     /label=-35
     promoter        181..186
                     /label=-10
     promoter        138..193
                     /label=BBa_K091111
     RBS             202..214
                     /label=RBS-3\Weak
     RBS             208..211
                     /label=RBS
     misc_feature    202..214
                     /label=BBa_B0032
     RBS             202..214
                     /label=BBa_B0032
     CDS             221..940
                     /label=GFP protein
     CDS             221..940
                     /label=BBa_E0040
     stem_loop       960..1003
                     /label=stem_loop
     misc_feature    949..1028
                     /label=BBa_B0010
     terminator      949..1028
                     /label=BBa_B0010
     stem_loop       1044..1063
                     /label=T7 TE
     misc_feature    1070..1070
                     /label=stop
     polyA_site      1064..1077
                     /label=polya
     misc_feature    1037..1077
                     /label=BBa_B0012
     terminator      1037..1077
                     /label=BBa_B0012
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagcgt tgacaccatc gaatggtgca aaacctttcg cggtatggca
      181 tgatagcgcc cggtactaga gtcacacagg aaagtactag atgcgtaaag gagaagaact
      241 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
      301 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
      361 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg
      421 tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc
      481 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa
      541 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
      601 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
      661 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat
      721 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc
      781 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct
      841 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc
      901 tgggattaca catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa
      961 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1021 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//