LOCUS       BBa_S04178              3439 bp    DNA     linear       05-Nov-2024
DEFINITION  K091222:K091221
ACCESSION   BBa_S04178
VERSION     BBa_S04178.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    1108..1146
                     /label=LVA
     CDS             19..1146
                     /label=lacI-LVA
     misc_feature    19..1146
                     /label=BBa_C0012
     misc_feature    1147..1171
                     /label=Help:Barcodes
     CDS             19..1146
                     /label=BBa_C0012
     misc_feature    1..1171
                     /label=BBa_S0100
     RBS             1180..1194
                     /label=RBS-1\Strong
     RBS             1187..1191
                     /label=RBS
     misc_feature    1180..1194
                     /label=BBa_B0030
     RBS             1180..1194
                     /label=BBa_B0030
     CDS             1201..2391
                     /label=tetA(C) forward
     primer_bind     1201..1224
                     /label=Fwd primer J31007
     primer_bind     2372..2391
                     /label=Rev primer J31007
     misc_feature    2389..2391
                     /label=stop
     CDS             1201..2391
                     /label=BBa_J31007
     stem_loop       2411..2454
                     /label=stem_loop
     misc_feature    2400..2479
                     /label=BBa_B0010
     terminator      2400..2479
                     /label=BBa_B0010
     stem_loop       2495..2514
                     /label=T7 TE
     misc_feature    2521..2521
                     /label=stop
     polyA_site      2515..2528
                     /label=polya
     misc_feature    2488..2528
                     /label=BBa_B0012
     terminator      2488..2528
                     /label=BBa_B0012
     misc_feature    1180..2528
                     /label=BBa_K091220
     misc_feature    1..2528
                     /label=BBa_K091222
     CDS             2537..3352
                     /label=Reverse of KanR gene
     CDS             2537..3352
                     /label=BBa_J31002
     RBS             3361..3375
                     /label=Reverse RBS
     RBS             3361..3375
                     /label=BBa_J44001
     promoter        3384..3439
                     /label=BBa_K091215
     misc_feature    2537..3439
                     /label=BBa_K091221
ORIGIN
        1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
       61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
      121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
      181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
      241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
      301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
      361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
      421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
      481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
      541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
      601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
      661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
      721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
      781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg
      841 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
      901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
      961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
     1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct
     1141 taataactct gatagtgcta gtgtagatct ctactagaga ttaaagagga gaaatactag
     1201 atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc
     1261 ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca ttccgacagc
     1321 atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca
     1381 cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta
     1441 cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gatcctctac
     1501 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
     1561 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
     1621 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
     1681 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
     1741 atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc
     1801 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
     1861 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc
     1921 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc
     1981 ctcgctcaag ccttcgtcac tggccccgcc accaaacgtt tcggcgagaa gcaggccatt
     2041 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc
     2101 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
     2161 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc
     2221 gcggctctta ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc gatttatgcc
     2281 gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc
     2341 tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgaccta atactagagc
     2401 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     2461 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     2521 cgtttatata ctagagttag aaaaactcat cgagcatcaa atgaaactgc aatttattca
     2581 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
     2641 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
     2701 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
     2761 caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga
     2821 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
     2881 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat
     2941 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
     3001 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg
     3061 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
     3121 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
     3181 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg
     3241 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
     3301 tggaatttaa tcgcggcctg gagcaagacg tttcccgttg aatatggctc attactagag
     3361 tttctcctct ttaattacta gagccgggcg ctatcatgcc ataccgcgaa aggtggtgtc
     3421 aacgtaaatg catgccgct
//