LOCUS BBa_S04178 3439 bp DNA linear 22-Dec-2024 DEFINITION K091222:K091221 ACCESSION BBa_S04178 VERSION BBa_S04178.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 1108..1146 /label=LVA CDS 19..1146 /label=lacI-LVA misc_feature 19..1146 /label=BBa_C0012 misc_feature 1147..1171 /label=Help:Barcodes CDS 19..1146 /label=BBa_C0012 misc_feature 1..1171 /label=BBa_S0100 RBS 1180..1194 /label=RBS-1\Strong RBS 1187..1191 /label=RBS misc_feature 1180..1194 /label=BBa_B0030 RBS 1180..1194 /label=BBa_B0030 CDS 1201..2391 /label=tetA(C) forward primer_bind 1201..1224 /label=Fwd primer J31007 primer_bind 2372..2391 /label=Rev primer J31007 misc_feature 2389..2391 /label=stop CDS 1201..2391 /label=BBa_J31007 stem_loop 2411..2454 /label=stem_loop misc_feature 2400..2479 /label=BBa_B0010 terminator 2400..2479 /label=BBa_B0010 stem_loop 2495..2514 /label=T7 TE misc_feature 2521..2521 /label=stop polyA_site 2515..2528 /label=polya misc_feature 2488..2528 /label=BBa_B0012 terminator 2488..2528 /label=BBa_B0012 misc_feature 1180..2528 /label=BBa_K091220 misc_feature 1..2528 /label=BBa_K091222 CDS 2537..3352 /label=Reverse of KanR gene CDS 2537..3352 /label=BBa_J31002 RBS 3361..3375 /label=Reverse RBS RBS 3361..3375 /label=BBa_J44001 promoter 3384..3439 /label=BBa_K091215 misc_feature 2537..3439 /label=BBa_K091221 ORIGIN 1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag 61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct 121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc 181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg 241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt 301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg 361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag 421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct 481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg 541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct 601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag 661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa 721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg 781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg 841 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag 901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag 961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg 1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct 1141 taataactct gatagtgcta gtgtagatct ctactagaga ttaaagagga gaaatactag 1201 atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc 1261 ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca ttccgacagc 1321 atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca 1381 cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta 1441 cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gatcctctac 1501 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc 1561 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc 1621 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat 1681 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta 1741 atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc 1801 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt 1861 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc 1921 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc 1981 ctcgctcaag ccttcgtcac tggccccgcc accaaacgtt tcggcgagaa gcaggccatt 2041 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc 2101 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg 2161 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc 2221 gcggctctta ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc gatttatgcc 2281 gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc 2341 tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgaccta atactagagc 2401 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 2461 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 2521 cgtttatata ctagagttag aaaaactcat cgagcatcaa atgaaactgc aatttattca 2581 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact 2641 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc 2701 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat 2761 caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga 2821 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt 2881 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat 2941 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt 3001 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg 3061 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa 3121 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt 3181 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg 3241 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt 3301 tggaatttaa tcgcggcctg gagcaagacg tttcccgttg aatatggctc attactagag 3361 tttctcctct ttaattacta gagccgggcg ctatcatgcc ataccgcgaa aggtggtgtc 3421 aacgtaaatg catgccgct //