LOCUS       BBa_S04182              1752 bp    DNA     linear       15-Dec-2024
DEFINITION  I0500:E0435
ACCESSION   BBa_S04182
VERSION     BBa_S04182.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     misc_feature    1237..1251
                     /label=lacZ gene fragment
     CDS             1237..1590
                     /label=lacZ alpha
     promoter        1262..1271
                     /label=T3 promoter
     promoter        1409..1427
                     /label=T7 promoter
     misc_feature    1314..1314
                     /label=C
     misc_feature    1317..1317
                     /label=T
     misc_feature    1323..1323
                     /label=A
     misc_feature    1344..1344
                     /label=C
     misc_feature    1347..1347
                     /label=G
     misc_feature    1435..1584
                     /label=lacZ gene fragment
     misc_feature    1584..1584
                     /label=inserted T
     misc_feature    1591..1615
                     /label=barcode
     misc_feature    1237..1615
                     /label=BBa_E0035
     stem_loop       1635..1678
                     /label=stem_loop
     misc_feature    1624..1703
                     /label=BBa_B0010
     terminator      1624..1703
                     /label=BBa_B0010
     stem_loop       1719..1738
                     /label=T7 TE
     misc_feature    1745..1745
                     /label=stop
     polyA_site      1739..1752
                     /label=polya
     misc_feature    1712..1752
                     /label=BBa_B0012
     terminator      1712..1752
                     /label=BBa_B0012
     terminator      1624..1752
                     /label=BBa_B0015
     misc_feature    1219..1752
                     /label=BBa_E0435
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga ccatgattac gccaagcgcg
     1261 caattaaccc tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggcagcacta
     1321 gagctagtgg atcccccggg ctgtagaaat tcgatatcaa gcttatcgat accgtcgacc
     1381 tcgagggggg gcccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     1441 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     1501 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     1561 tcccaacagt tgcgcagcct gaattaataa cgctgatagt gctagtgtag atcgctacta
     1621 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1681 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1741 tctgcgttta ta
//