##gff-version 3
##sequence-region BBa_S04182 1 1752
BBa_S04182	.	engineered_region	1219	1752	.	+	0	ID=BBa_E0435;Name=BBa_E0435
BBa_S04182	.	ribosome_entry_site	1219	1230	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0435
BBa_S04182	.	conserved_region	1223	1226	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04182	.	terminator	1624	1752	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_E0435
BBa_S04182	.	terminator	1624	1703	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04182	.	engineered_region	1624	1703	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04182	.	stem_loop	1635	1678	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04182	.	terminator	1712	1752	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04182	.	engineered_region	1712	1752	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04182	.	stem_loop	1719	1738	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04182	.	polyA_site	1739	1752	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04182	.	stop_codon	1745	1745	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04182	.	engineered_region	1237	1615	.	+	0	ID=BBa_E0035;Name=BBa_E0035;Parent=BBa_E0435
BBa_S04182	.	sequence_alteration	1344	1344	.	+	0	ID=annotation1959042;Name=C;Parent=BBa_E0035
BBa_S04182	.	sequence_alteration	1347	1347	.	+	0	ID=annotation1959043;Name=G;Parent=BBa_E0035
BBa_S04182	.	sequence_alteration	1584	1584	.	+	0	ID=annotation1959045;Name=inserted T;Parent=BBa_E0035
BBa_S04182	.	sequence_alteration	1314	1314	.	+	0	ID=annotation1959039;Name=C;Parent=BBa_E0035
BBa_S04182	.	engineered_tag	1591	1615	.	+	0	ID=annotation1959046;Name=barcode;Parent=BBa_E0035
BBa_S04182	.	sequence_feature	1435	1584	.	+	0	ID=annotation1959044;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_S04182	.	sequence_alteration	1323	1323	.	+	0	ID=annotation1959041;Name=A;Parent=BBa_E0035
BBa_S04182	.	sequence_alteration	1317	1317	.	+	0	ID=annotation1959040;Name=T;Parent=BBa_E0035
BBa_S04182	.	promoter	1262	1271	.	+	0	ID=annotation1959037;Name=T3 promoter;Parent=BBa_E0035
BBa_S04182	.	promoter	1409	1427	.	+	0	ID=annotation1959038;Name=T7 promoter;Parent=BBa_E0035
BBa_S04182	.	CDS	1237	1590	.	+	0	ID=annotation1959036;Name=lacZ alpha;Parent=BBa_E0035
BBa_S04182	.	sequence_feature	1237	1251	.	+	0	ID=annotation1959035;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_S04182	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_S04182	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_S04182	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_S04182	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
>BBa_S04182
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagaaagaggagaaatactagatgaccatgattacgccaagcgcgcaat
taaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctg
tagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcg
tattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttg
cagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcct
gaattaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttata