LOCUS       BBa_S04183              1664 bp    DNA     linear       16-Dec-2024
DEFINITION  J04450:I6413
ACCESSION   BBa_S04183
VERSION     BBa_S04183.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..901
                     /label=mrfp1
     misc_feature    908..932
                     /label=Help:Barcodes
     CDS             227..907
                     /label=BBa_E1010
     stem_loop       952..995
                     /label=stem_loop
     misc_feature    941..1020
                     /label=BBa_B0010
     terminator      941..1020
                     /label=BBa_B0010
     stem_loop       1036..1055
                     /label=T7 TE
     misc_feature    1062..1062
                     /label=stop
     polyA_site      1056..1069
                     /label=polya
     misc_feature    1029..1069
                     /label=BBa_B0012
     terminator      1029..1069
                     /label=BBa_B0012
     misc_feature    1..1069
                     /label=BBa_J04450
     promoter        1087..1092
                     /label=-35
     promoter        1110..1115
                     /label=-10
     misc_feature    1097..1110
                     /label=Zif23
     misc_feature    1078..1086
                     /label=consensus
     misc_feature    1122..1122
                     /label=(+1)
     promoter        1078..1122
                     /label=BBa_R2000
     misc_feature    1135..1138
                     /label=conserved
     RBS             1131..1142
                     /label=BBa_B0034
     misc_feature    1149..1163
                     /label=lacZ gene fragment
     CDS             1149..1502
                     /label=lacZ alpha
     promoter        1174..1183
                     /label=T3 promoter
     promoter        1321..1339
                     /label=T7 promoter
     misc_feature    1226..1226
                     /label=C
     misc_feature    1229..1229
                     /label=T
     misc_feature    1235..1235
                     /label=A
     misc_feature    1256..1256
                     /label=C
     misc_feature    1259..1259
                     /label=G
     misc_feature    1347..1496
                     /label=lacZ gene fragment
     misc_feature    1496..1496
                     /label=inserted T
     misc_feature    1503..1527
                     /label=barcode
     misc_feature    1149..1527
                     /label=BBa_E0035
     stem_loop       1547..1590
                     /label=stem_loop
     misc_feature    1536..1615
                     /label=BBa_B0010
     terminator      1536..1615
                     /label=BBa_B0010
     stem_loop       1631..1650
                     /label=T7 TE
     misc_feature    1657..1657
                     /label=stop
     polyA_site      1651..1664
                     /label=polya
     misc_feature    1624..1664
                     /label=BBa_B0012
     terminator      1624..1664
                     /label=BBa_B0012
     terminator      1536..1664
                     /label=BBa_B0015
     misc_feature    1131..1664
                     /label=BBa_E0435
     misc_feature    1078..1664
                     /label=BBa_I6413
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga
      961 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1021 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagagt
     1081 ttattcttga catggtccca cgcgcgtggg atactacgtc agtactagag aaagaggaga
     1141 aatactagat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     1201 gctggagctc caccgcggtg gcggcagcac tagagctagt ggatcccccg ggctgtagaa
     1261 attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta cccaattcgc
     1321 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     1381 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     1441 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaattaat
     1501 aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc
     1561 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1621 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//