##gff-version 3
##sequence-region BBa_S04183 1 1664
BBa_S04183	.	engineered_region	1078	1664	.	+	0	ID=BBa_I6413;Name=BBa_I6413
BBa_S04183	.	promoter	1078	1122	.	+	0	ID=BBa_R2000;Name=BBa_R2000;Parent=BBa_I6413
BBa_S04183	.	start_codon	1122	1122	.	+	0	ID=annotation318102;Name=(+1);Parent=BBa_R2000
BBa_S04183	.	promoter	1110	1115	.	+	0	ID=annotation318098;Name=-10;Parent=BBa_R2000
BBa_S04183	.	non_covalent_binding_site	1097	1110	.	+	0	ID=annotation318099;Name=Zif23;Parent=BBa_R2000
BBa_S04183	.	sequence_feature	1078	1086	.	+	0	ID=annotation318100;Name=consensus;Parent=BBa_R2000
BBa_S04183	.	promoter	1087	1092	.	+	0	ID=annotation318094;Name=-35;Parent=BBa_R2000
BBa_S04183	.	engineered_region	1131	1664	.	+	0	ID=BBa_E0435;Name=BBa_E0435;Parent=BBa_I6413
BBa_S04183	.	engineered_region	1149	1527	.	+	0	ID=BBa_E0035;Name=BBa_E0035;Parent=BBa_E0435
BBa_S04183	.	sequence_alteration	1259	1259	.	+	0	ID=annotation1959043;Name=G;Parent=BBa_E0035
BBa_S04183	.	CDS	1149	1502	.	+	0	ID=annotation1959036;Name=lacZ alpha;Parent=BBa_E0035
BBa_S04183	.	sequence_feature	1347	1496	.	+	0	ID=annotation1959044;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_S04183	.	promoter	1321	1339	.	+	0	ID=annotation1959038;Name=T7 promoter;Parent=BBa_E0035
BBa_S04183	.	sequence_alteration	1235	1235	.	+	0	ID=annotation1959041;Name=A;Parent=BBa_E0035
BBa_S04183	.	sequence_alteration	1496	1496	.	+	0	ID=annotation1959045;Name=inserted T;Parent=BBa_E0035
BBa_S04183	.	sequence_alteration	1256	1256	.	+	0	ID=annotation1959042;Name=C;Parent=BBa_E0035
BBa_S04183	.	sequence_alteration	1226	1226	.	+	0	ID=annotation1959039;Name=C;Parent=BBa_E0035
BBa_S04183	.	sequence_alteration	1229	1229	.	+	0	ID=annotation1959040;Name=T;Parent=BBa_E0035
BBa_S04183	.	promoter	1174	1183	.	+	0	ID=annotation1959037;Name=T3 promoter;Parent=BBa_E0035
BBa_S04183	.	engineered_tag	1503	1527	.	+	0	ID=annotation1959046;Name=barcode;Parent=BBa_E0035
BBa_S04183	.	sequence_feature	1149	1163	.	+	0	ID=annotation1959035;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_S04183	.	ribosome_entry_site	1131	1142	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0435
BBa_S04183	.	conserved_region	1135	1138	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04183	.	terminator	1536	1664	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_E0435
BBa_S04183	.	terminator	1536	1615	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04183	.	stem_loop	1547	1590	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04183	.	engineered_region	1536	1615	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04183	.	terminator	1624	1664	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04183	.	engineered_region	1624	1664	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04183	.	stop_codon	1657	1657	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04183	.	polyA_site	1651	1664	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04183	.	stem_loop	1631	1650	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04183	.	engineered_region	1	1069	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_S04183	.	terminator	1029	1069	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_S04183	.	engineered_region	1029	1069	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04183	.	stop_codon	1062	1062	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04183	.	polyA_site	1056	1069	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04183	.	stem_loop	1036	1055	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04183	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_S04183	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S04183	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S04183	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S04183	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S04183	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S04183	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S04183	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S04183	.	CDS	227	907	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_S04183	.	CDS	227	901	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_S04183	.	engineered_tag	908	932	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_S04183	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_S04183	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04183	.	terminator	941	1020	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_S04183	.	stem_loop	952	995	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04183	.	engineered_region	941	1020	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_S04183
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctc
cgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaa
ggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcg
cttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacct
gaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag
gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggtta
tgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaa
aatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcag
ctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacg
aacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagtttattcttgacatggtcccacgcgcg
tgggatactacgtcagtactagagaaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaaccctca
ctaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattc
gatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcg
cgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatc
cccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaattaata
acgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct
ttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgt
ttata