LOCUS       BBa_S04184              1664 bp    DNA     linear       15-Dec-2024
DEFINITION  I6413:J04450
ACCESSION   BBa_S04184
VERSION     BBa_S04184.1  
FEATURES             Location/Qualifiers
     promoter        10..15
                     /label=-35
     promoter        33..38
                     /label=-10
     misc_feature    20..33
                     /label=Zif23
     misc_feature    1..9
                     /label=consensus
     misc_feature    45..45
                     /label=(+1)
     promoter        1..45
                     /label=BBa_R2000
     misc_feature    58..61
                     /label=conserved
     RBS             54..65
                     /label=BBa_B0034
     misc_feature    72..86
                     /label=lacZ gene fragment
     CDS             72..425
                     /label=lacZ alpha
     promoter        97..106
                     /label=T3 promoter
     promoter        244..262
                     /label=T7 promoter
     misc_feature    149..149
                     /label=C
     misc_feature    152..152
                     /label=T
     misc_feature    158..158
                     /label=A
     misc_feature    179..179
                     /label=C
     misc_feature    182..182
                     /label=G
     misc_feature    270..419
                     /label=lacZ gene fragment
     misc_feature    419..419
                     /label=inserted T
     misc_feature    426..450
                     /label=barcode
     misc_feature    72..450
                     /label=BBa_E0035
     stem_loop       470..513
                     /label=stem_loop
     misc_feature    459..538
                     /label=BBa_B0010
     terminator      459..538
                     /label=BBa_B0010
     stem_loop       554..573
                     /label=T7 TE
     misc_feature    580..580
                     /label=stop
     polyA_site      574..587
                     /label=polya
     misc_feature    547..587
                     /label=BBa_B0012
     terminator      547..587
                     /label=BBa_B0012
     terminator      459..587
                     /label=BBa_B0015
     misc_feature    54..587
                     /label=BBa_E0435
     misc_feature    1..587
                     /label=BBa_I6413
     CDS             596..683
                     /label=end of LacI coding region (inactive)
     misc_feature    596..795
                     /label=BBa_R0010
     misc_feature    684..721
                     /label=CAP binding site
     promoter        732..737
                     /label=-35
     promoter        756..761
                     /label=-10
     misc_feature    761..795
                     /label=LacI binding site
     misc_feature    768..768
                     /label=start
     promoter        596..795
                     /label=BBa_R0010
     misc_feature    808..811
                     /label=conserved
     RBS             804..815
                     /label=BBa_B0034
     CDS             822..1496
                     /label=mrfp1
     misc_feature    1503..1527
                     /label=Help:Barcodes
     CDS             822..1502
                     /label=BBa_E1010
     stem_loop       1547..1590
                     /label=stem_loop
     misc_feature    1536..1615
                     /label=BBa_B0010
     terminator      1536..1615
                     /label=BBa_B0010
     stem_loop       1631..1650
                     /label=T7 TE
     misc_feature    1657..1657
                     /label=stop
     polyA_site      1651..1664
                     /label=polya
     misc_feature    1624..1664
                     /label=BBa_B0012
     terminator      1624..1664
                     /label=BBa_B0012
     misc_feature    596..1664
                     /label=BBa_J04450
ORIGIN
        1 agtttattct tgacatggtc ccacgcgcgt gggatactac gtcagtacta gagaaagagg
       61 agaaatacta gatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
      121 aaagctggag ctccaccgcg gtggcggcag cactagagct agtggatccc ccgggctgta
      181 gaaattcgat atcaagctta tcgataccgt cgacctcgag ggggggcccg gtacccaatt
      241 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
      301 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      361 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatt
      421 aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa
      481 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      541 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagcaata
      601 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
      661 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
      721 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
      781 taacaatttc acacatacta gagaaagagg agaaatacta gatggcttcc tccgaagacg
      841 ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt
      901 tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga
      961 aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt
     1021 acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct
     1081 tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg
     1141 ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta
     1201 ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca
     1261 ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac
     1321 tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg
     1381 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag
     1441 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat
     1501 aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc
     1561 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1621 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//