BBa_S04184
type: ComponentDefinition
persistent identity: BBa S04184
display id: BBa_S04184
version: 1
was generated by: iGEM to SBOL conversion
description: I6413:J04450
created: 2009-03-23T12:00:00Z
modified: 2015-05-08T01:14:36Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _84_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J04450
component: BBa_I6413
sequence annotation: BBa_I6413
sequence annotation: BBa_J04450
sequence: https://synbiohub.org/public/igem/BBa_S04184_sequence/1

BBa_J04450
type: Component
persistent identity: component2283321
display id: component2283321
version: 1
top level: BBa_S04184
definition: BBa_J04450
access: public

BBa_I6413
type: Component
persistent identity: component2283303
display id: component2283303
version: 1
top level: BBa_S04184
definition: BBa_I6413
access: public

BBa_I6413
type: SequenceAnnotation
persistent identity: annotation2283303
display id: annotation2283303
version: 1
top level: BBa_S04184
location: https://synbiohub.org/public/igem/BBa_S04184/annotation2283303/range2283303/1
component: BBa_I6413

https://synbiohub.org/public/igem/BBa_S04184/annotation2283303/range2283303/1
type: Range
persistent identity: range2283303
display id: range2283303
version: 1
top level: BBa_S04184
direction: direction/reverse
start: 1
end: 587
orientation: inline

BBa_J04450
type: SequenceAnnotation
persistent identity: annotation2283321
display id: annotation2283321
version: 1
top level: BBa_S04184
location: https://synbiohub.org/public/igem/BBa_S04184/annotation2283321/range2283321/1
component: BBa_J04450

https://synbiohub.org/public/igem/BBa_S04184/annotation2283321/range2283321/1
type: Range
persistent identity: range2283321
display id: range2283321
version: 1
top level: BBa_S04184
direction: direction/reverse
start: 596
end: 1664
orientation: inline

BBa_I6413
type: ComponentDefinition
persistent identity: BBa I6413
display id: BBa_I6413
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter high output protein generator
created: 2008-01-09T12:00:00Z
modified: 2015-08-31T04:07:44Z
mutable provenance: This is a composite part.
owned by: james
owned by: myers
mutable description: Produces lacZalpha constitutively in the absence of BBa_C2006 repressor.
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Measurement
role: SO:0000804
component: BBa_R2000
component: BBa_E0435
sequence annotation: BBa_R2000
sequence annotation: BBa_E0435
sequence: https://synbiohub.org/public/igem/BBa_I6413_sequence/1

BBa_R2000
type: Component
persistent identity: component2253498
display id: component2253498
version: 1
top level: BBa_I6413
definition: Zif23
access: public

BBa_E0435
type: Component
persistent identity: component2253521
display id: component2253521
version: 1
top level: BBa_I6413
definition: BBa_E0435
access: public

BBa_R2000
type: SequenceAnnotation
persistent identity: annotation2253498
display id: annotation2253498
version: 1
top level: BBa_I6413
location: https://synbiohub.org/public/igem/BBa_I6413/annotation2253498/range2253498/1
component: BBa_R2000

https://synbiohub.org/public/igem/BBa_I6413/annotation2253498/range2253498/1
type: Range
persistent identity: range2253498
display id: range2253498
version: 1
top level: BBa_I6413
direction: direction/reverse
start: 1
end: 45
orientation: inline

BBa_E0435
type: SequenceAnnotation
persistent identity: annotation2253521
display id: annotation2253521
version: 1
top level: BBa_I6413
location: https://synbiohub.org/public/igem/BBa_I6413/annotation2253521/range2253521/1
component: BBa_E0435

https://synbiohub.org/public/igem/BBa_I6413/annotation2253521/range2253521/1
type: Range
persistent identity: range2253521
display id: range2253521
version: 1
top level: BBa_I6413
direction: direction/reverse
start: 54
end: 587
orientation: inline

BBa_J04450
type: ComponentDefinition
persistent identity: BBa J04450
display id: BBa_J04450
version: 1
was generated by: iGEM to SBOL conversion
description: RFP Coding Device
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Contains an IPTG inducible promoter an RBS, RFP, and terminator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 328
owning group id: 16
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Tamar Odle
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0034
component: BBa_R0010
component: BBa_B0012
component: BBa_B0010
component: BBa_E1010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J04450_sequence/1

BBa_B0034
type: Component
persistent identity: component1509404
display id: component1509404
version: 1
top level: BBa_J04450
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component1509394
display id: component1509394
version: 1
top level: BBa_J04450
definition: LacI
access: public

BBa_B0012
type: Component
persistent identity: component1509427
display id: component1509427
version: 1
top level: BBa_J04450
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1509417
display id: component1509417
version: 1
top level: BBa_J04450
definition: BBa_B0010
access: public

BBa_E1010
type: Component
persistent identity: component1509411
display id: component1509411
version: 1
top level: BBa_J04450
definition: mRFP1
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1509394
display id: annotation1509394
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
type: Range
persistent identity: range1509394
display id: range1509394
version: 1
top level: BBa_J04450
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1509404
display id: annotation1509404
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
type: Range
persistent identity: range1509404
display id: range1509404
version: 1
top level: BBa_J04450
direction: direction/forward
start: 209
end: 220
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1509411
display id: annotation1509411
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
type: Range
persistent identity: range1509411
display id: range1509411
version: 1
top level: BBa_J04450
direction: direction/forward
start: 227
end: 907
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1509417
display id: annotation1509417
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
type: Range
persistent identity: range1509417
display id: range1509417
version: 1
top level: BBa_J04450
direction: direction/forward
start: 941
end: 1020
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1509427
display id: annotation1509427
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
type: Range
persistent identity: range1509427
display id: range1509427
version: 1
top level: BBa_J04450
direction: direction/forward
start: 1029
end: 1069
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_E0435
type: ComponentDefinition
persistent identity: BBa E0435
display id: BBa_E0435
version: 1
was generated by: iGEM to SBOL conversion
description: PoPS->lacZalpha reporter device
created: 2007-12-21T12:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: It is derived from BBa_E0433.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This device takes a PoPS signal in and produces the protein lacZalpha. When put in a strain that can provide alpha-complementation, you should see measurable beta-galactosidase activity.
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An additional T was inserted to ensure that the double stop codon is in frame with the rest of the protein.
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_E0035
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_E0035
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_E0435_sequence/1

BBa_B0015
type: Component
persistent identity: component2249043
display id: component2249043
version: 1
top level: BBa_E0435
definition: BBa_B0015
access: public

BBa_E0035
type: Component
persistent identity: component2249036
display id: component2249036
version: 1
top level: BBa_E0435
definition: BBa_E0035
access: public

BBa_B0034
type: Component
persistent identity: component2249023
display id: component2249023
version: 1
top level: BBa_E0435
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2249023
display id: annotation2249023
version: 1
top level: BBa_E0435
location: https://synbiohub.org/public/igem/BBa_E0435/annotation2249023/range2249023/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0435/annotation2249023/range2249023/1
type: Range
persistent identity: range2249023
display id: range2249023
version: 1
top level: BBa_E0435
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E0035
type: SequenceAnnotation
persistent identity: annotation2249036
display id: annotation2249036
version: 1
top level: BBa_E0435
location: https://synbiohub.org/public/igem/BBa_E0435/annotation2249036/range2249036/1
component: BBa_E0035

https://synbiohub.org/public/igem/BBa_E0435/annotation2249036/range2249036/1
type: Range
persistent identity: range2249036
display id: range2249036
version: 1
top level: BBa_E0435
direction: direction/reverse
start: 19
end: 397
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2249043
display id: annotation2249043
version: 1
top level: BBa_E0435
location: https://synbiohub.org/public/igem/BBa_E0435/annotation2249043/range2249043/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_E0435/annotation2249043/range2249043/1
type: Range
persistent identity: range2249043
display id: range2249043
version: 1
top level: BBa_E0435
direction: direction/reverse
start: 406
end: 534
orientation: inline

Zif23
type: ComponentDefinition
persistent identity: BBa R2000
display id: BBa_R2000
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter, Zif23 regulated, test: between
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: synthetic
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter with standard -10 and -35 sites. A symmetric Zif23 dimer target site is between the -10 and -35 sites.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: consensus sequences based on [Jensen98].
star: true
creator: Ronny Krashinsky
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: consensus
sequence annotation: -35
sequence annotation: Zif23
sequence annotation: -10
sequence annotation: (+1)
sequence: https://synbiohub.org/public/igem/BBa_R2000_sequence/1

consensus
type: SequenceAnnotation
persistent identity: annotation318100
display id: annotation318100
version: 1
top level: Zif23
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318100/range318100/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R2000/annotation318100/range318100/1
type: Range
persistent identity: range318100
display id: range318100
version: 1
top level: Zif23
direction: direction/forward
start: 1
end: 9
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation318094
display id: annotation318094
version: 1
top level: Zif23
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318094/range318094/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R2000/annotation318094/range318094/1
type: Range
persistent identity: range318094
display id: range318094
version: 1
top level: Zif23
direction: direction/forward
start: 10
end: 15
orientation: inline

Zif23
type: SequenceAnnotation
persistent identity: annotation318099
display id: annotation318099
version: 1
top level: Zif23
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318099/range318099/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R2000/annotation318099/range318099/1
type: Range
persistent identity: range318099
display id: range318099
version: 1
top level: Zif23
direction: direction/forward
start: 20
end: 33
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318098
display id: annotation318098
version: 1
top level: Zif23
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318098/range318098/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R2000/annotation318098/range318098/1
type: Range
persistent identity: range318098
display id: range318098
version: 1
top level: Zif23
direction: direction/forward
start: 33
end: 38
orientation: inline

(+1)
type: SequenceAnnotation
persistent identity: annotation318102
display id: annotation318102
version: 1
top level: Zif23
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318102/range318102/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R2000/annotation318102/range318102/1
type: Range
persistent identity: range318102
display id: range318102
version: 1
top level: Zif23
direction: direction/forward
start: 45
end: 45
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_E0035
type: ComponentDefinition
persistent identity: BBa E0035
display id: BBa_E0035
version: 1
was generated by: iGEM to SBOL conversion
description: LacZ alpha fragment; complements matching N-terminal deletion mutant (lacZ-omega)
created: 2007-12-21T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: This part is derived from BBa_E0033.
owned by: james
owned by: myers
mutable description: This part encodes the alpha fragment of the lacZ gene. To greatly reduce the number of bases, this is only a portion of the LacZ gene. It can be used as a detection system with N-terminal deletion mutants of lacZ. Combination of lacZ-alpha with such a deletion mutant protein restores enzyme activity that can be assayed colorimetrically or more sensitively with chemiluminescent substrates.
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: It has the additional T needed to ensure that the double stop codon is in frame.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: lacZ gene fragment
sequence annotation: lacZ alpha
sequence annotation: T3 promoter
sequence annotation: C
sequence annotation: T
sequence annotation: A
sequence annotation: G
sequence annotation: T7 promoter
sequence annotation: inserted T
sequence annotation: barcode
sequence: https://synbiohub.org/public/igem/BBa_E0035_sequence/1

lacZ gene fragment
type: SequenceAnnotation
persistent identity: annotation1959035
display id: annotation1959035
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959035/range1959035/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_E0035/annotation1959035/range1959035/1
type: Range
persistent identity: range1959035
display id: range1959035
version: 1
top level: BBa_E0035
direction: direction/forward
start: 1
end: 15
orientation: inline

lacZ alpha
type: SequenceAnnotation
persistent identity: annotation1959036
display id: annotation1959036
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959036/range1959036/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0035/annotation1959036/range1959036/1
type: Range
persistent identity: range1959036
display id: range1959036
version: 1
top level: BBa_E0035
direction: direction/forward
start: 1
end: 354
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1959037
display id: annotation1959037
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959037/range1959037/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_E0035/annotation1959037/range1959037/1
type: Range
persistent identity: range1959037
display id: range1959037
version: 1
top level: BBa_E0035
direction: direction/forward
start: 26
end: 35
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation1959039
display id: annotation1959039
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959039/range1959039/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_E0035/annotation1959039/range1959039/1
type: Range
persistent identity: range1959039
display id: range1959039
version: 1
top level: BBa_E0035
direction: direction/forward
start: 78
end: 78
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1959040
display id: annotation1959040
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959040/range1959040/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_E0035/annotation1959040/range1959040/1
type: Range
persistent identity: range1959040
display id: range1959040
version: 1
top level: BBa_E0035
direction: direction/forward
start: 81
end: 81
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1959041
display id: annotation1959041
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959041/range1959041/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0035/annotation1959041/range1959041/1
type: Range
persistent identity: range1959041
display id: range1959041
version: 1
top level: BBa_E0035
direction: direction/forward
start: 87
end: 87
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation1959042
display id: annotation1959042
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959042/range1959042/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0035/annotation1959042/range1959042/1
type: Range
persistent identity: range1959042
display id: range1959042
version: 1
top level: BBa_E0035
direction: direction/forward
start: 108
end: 108
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation1959043
display id: annotation1959043
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959043/range1959043/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0035/annotation1959043/range1959043/1
type: Range
persistent identity: range1959043
display id: range1959043
version: 1
top level: BBa_E0035
direction: direction/forward
start: 111
end: 111
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1959038
display id: annotation1959038
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959038/range1959038/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_E0035/annotation1959038/range1959038/1
type: Range
persistent identity: range1959038
display id: range1959038
version: 1
top level: BBa_E0035
direction: direction/forward
start: 173
end: 191
orientation: inline

lacZ gene fragment
type: SequenceAnnotation
persistent identity: annotation1959044
display id: annotation1959044
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959044/range1959044/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_E0035/annotation1959044/range1959044/1
type: Range
persistent identity: range1959044
display id: range1959044
version: 1
top level: BBa_E0035
direction: direction/forward
start: 199
end: 348
orientation: inline

inserted T
type: SequenceAnnotation
persistent identity: annotation1959045
display id: annotation1959045
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959045/range1959045/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0035/annotation1959045/range1959045/1
type: Range
persistent identity: range1959045
display id: range1959045
version: 1
top level: BBa_E0035
direction: direction/forward
start: 348
end: 348
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation1959046
display id: annotation1959046
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959046/range1959046/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_E0035/annotation1959046/range1959046/1
type: Range
persistent identity: range1959046
display id: range1959046
version: 1
top level: BBa_E0035
direction: direction/forward
start: 355
end: 379
orientation: inline

https://synbiohub.org/public/igem/BBa_S04184_sequence/1
type: Sequence
persistent identity: BBa S04184 sequence
display id: BBa_S04184_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtttattcttgacatggtcccacgcgcgtgggatactacgtcagtactagagaaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaattaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I6413_sequence/1
type: Sequence
persistent identity: BBa I6413 sequence
display id: BBa_I6413_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtttattcttgacatggtcccacgcgcgtgggatactacgtcagtactagagaaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaattaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J04450_sequence/1
type: Sequence
persistent identity: BBa J04450 sequence
display id: BBa_J04450_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0435_sequence/1
type: Sequence
persistent identity: BBa E0435 sequence
display id: BBa_E0435_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaattaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R2000_sequence/1
type: Sequence
persistent identity: BBa R2000 sequence
display id: BBa_R2000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtttattcttgacatggtcccacgcgcgtgggatactacgtcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0035_sequence/1
type: Sequence
persistent identity: BBa E0035 sequence
display id: BBa_E0035_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaattaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z