LOCUS       BBa_S04185              2015 bp    DNA     linear       15-Dec-2024
DEFINITION  I7101:J04450
ACCESSION   BBa_S04185
VERSION     BBa_S04185.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     CDS             82..801
                     /label=GFP protein
     CDS             82..801
                     /label=BBa_E0040
     stem_loop       821..864
                     /label=stem_loop
     misc_feature    810..889
                     /label=BBa_B0010
     terminator      810..889
                     /label=BBa_B0010
     stem_loop       905..924
                     /label=T7 TE
     misc_feature    931..931
                     /label=stop
     polyA_site      925..938
                     /label=polya
     misc_feature    898..938
                     /label=BBa_B0012
     terminator      898..938
                     /label=BBa_B0012
     misc_feature    1..938
                     /label=BBa_I7101
     CDS             947..1034
                     /label=end of LacI coding region (inactive)
     misc_feature    947..1146
                     /label=BBa_R0010
     misc_feature    1035..1072
                     /label=CAP binding site
     promoter        1083..1088
                     /label=-35
     promoter        1107..1112
                     /label=-10
     misc_feature    1112..1146
                     /label=LacI binding site
     misc_feature    1119..1119
                     /label=start
     promoter        947..1146
                     /label=BBa_R0010
     misc_feature    1159..1162
                     /label=conserved
     RBS             1155..1166
                     /label=BBa_B0034
     CDS             1173..1847
                     /label=mrfp1
     misc_feature    1854..1878
                     /label=Help:Barcodes
     CDS             1173..1853
                     /label=BBa_E1010
     stem_loop       1898..1941
                     /label=stem_loop
     misc_feature    1887..1966
                     /label=BBa_B0010
     terminator      1887..1966
                     /label=BBa_B0010
     stem_loop       1982..2001
                     /label=T7 TE
     misc_feature    2008..2008
                     /label=stop
     polyA_site      2002..2015
                     /label=polya
     misc_feature    1975..2015
                     /label=BBa_B0012
     terminator      1975..2015
                     /label=BBa_B0012
     misc_feature    947..2015
                     /label=BBa_J04450
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagtacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
      121 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      181 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      241 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      301 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      361 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      421 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      481 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      541 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      601 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      661 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      721 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
      781 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc
      841 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      901 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagcaat acgcaaaccg
      961 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     1021 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     1081 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     1141 cacacatact agagaaagag gagaaatact agatggcttc ctccgaagac gttatcaaag
     1201 agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg
     1261 aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca
     1321 aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca
     1381 aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag
     1441 gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg
     1501 actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc
     1561 cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta
     1621 tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg
     1681 gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc
     1741 cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca
     1801 tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taacgctgat
     1861 agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     1921 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1981 tggctcacct tcgggtgggc ctttctgcgt ttata
//