##gff-version 3
##sequence-region BBa_S04186 1 3736
BBa_S04186	.	CDS	2961	3680	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_S04186	.	CDS	2961	3680	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_S04186	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_S04186	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S04186	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S04186	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S04186	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S04186	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S04186	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S04186	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S04186	.	promoter	2890	2933	.	+	0	ID=BBa_R0184;Name=BBa_R0184
BBa_S04186	.	operator	2909	2933	.	+	0	ID=annotation1932679;Name=lacI binding site;Parent=BBa_R0184
BBa_S04186	.	promoter	2890	2909	.	+	0	ID=annotation1932678;Name=BBa_R0183 fragment;Parent=BBa_R0184
BBa_S04186	.	sequence_feature	2920	2920	.	+	0	ID=annotation1932680;Name=Center of binding site symmetry;Parent=BBa_R0184
BBa_S04186	.	terminator	3689	3736	.	+	0	ID=BBa_B0016;Name=BBa_B0016
BBa_S04186	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_S04186	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04186	.	CDS	227	2881	.	+	0	ID=BBa_I2032;Name=BBa_I2032
BBa_S04186	.	promoter	2724	2751	.	+	0	ID=annotation1928674;Name=putative E. coli promoter;Parent=BBa_I2032
BBa_S04186	.	ribosome_entry_site	2942	2954	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_S04186	.	ribosome_entry_site	2948	2951	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04186	.	engineered_region	2942	2954	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04186	.	ribosome_entry_site	2942	2954	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
>BBa_S04186
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaacacgat
taacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgag
cgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagc
gtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgc
acgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatc
aagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcagg
ctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcactt
caagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggct
gacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtac
gctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactc
tgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatg
ttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctc
tggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgat
taacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcat
tgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgagg
ctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttga
gttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgt
gtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaa
tcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcg
catcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgag
caagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgct
cccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcg
cgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaa
gcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtca
agctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgac
gctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggc
aagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacgg
tggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagac
tggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaag
cctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcg
agattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagac
tgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgac
gctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacg
accagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccg
tgacatcttagagtcggacttcgcgttcgcgtaataatactagagtcatacgactcactataggggaattgtgagcgga
taacaattcctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaatt
cttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaa
aacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatgg
tgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgta
caggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttg
ttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataa
ctcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaa
gatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaacc
attacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaac
agctgctgggattacacatggcatggatgaactatacaaataataatactagagctagcataaccccttggggcctcta
aacgggtcttgaggggttttttg