BBa_S04186
type: ComponentDefinition
persistent identity: BBa S04186
display id: BBa_S04186
version: 1
was generated by: iGEM to SBOL conversion
description: I715038:I2035
created: 2009-03-23T12:00:00Z
modified: 2015-05-08T01:14:36Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _84_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0016
component: BBa_E0040
component: BBa_B0032
component: BBa_R0184
component: BBa_I2032
component: BBa_B0034
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_I2032
sequence annotation: BBa_R0184
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0016
sequence: https://synbiohub.org/public/igem/BBa_S04186_sequence/1

BBa_B0016
type: Component
persistent identity: component2002037
display id: component2002037
version: 1
top level: BBa_S04186
definition: BBa_B0016
access: public

BBa_E0040
type: Component
persistent identity: component2002036
display id: component2002036
version: 1
top level: BBa_S04186
definition: GFP
access: public

BBa_B0032
type: Component
persistent identity: component2002033
display id: component2002033
version: 1
top level: BBa_S04186
definition: BBa_B0032
access: public

BBa_R0184
type: Component
persistent identity: component2002031
display id: component2002031
version: 1
top level: BBa_S04186
definition: BBa_R0184
access: public

BBa_I2032
type: Component
persistent identity: component2002027
display id: component2002027
version: 1
top level: BBa_S04186
definition: BBa_I2032
access: public

BBa_B0034
type: Component
persistent identity: component2002025
display id: component2002025
version: 1
top level: BBa_S04186
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component2002017
display id: component2002017
version: 1
top level: BBa_S04186
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2002017
display id: annotation2002017
version: 1
top level: BBa_S04186
location: https://synbiohub.org/public/igem/BBa_S04186/annotation2002017/range2002017/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_S04186/annotation2002017/range2002017/1
type: Range
persistent identity: range2002017
display id: range2002017
version: 1
top level: BBa_S04186
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2002025
display id: annotation2002025
version: 1
top level: BBa_S04186
location: https://synbiohub.org/public/igem/BBa_S04186/annotation2002025/range2002025/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04186/annotation2002025/range2002025/1
type: Range
persistent identity: range2002025
display id: range2002025
version: 1
top level: BBa_S04186
direction: direction/reverse
start: 209
end: 220
orientation: inline

BBa_I2032
type: SequenceAnnotation
persistent identity: annotation2002027
display id: annotation2002027
version: 1
top level: BBa_S04186
location: https://synbiohub.org/public/igem/BBa_S04186/annotation2002027/range2002027/1
component: BBa_I2032

https://synbiohub.org/public/igem/BBa_S04186/annotation2002027/range2002027/1
type: Range
persistent identity: range2002027
display id: range2002027
version: 1
top level: BBa_S04186
direction: direction/reverse
start: 227
end: 2881
orientation: inline

BBa_R0184
type: SequenceAnnotation
persistent identity: annotation2002031
display id: annotation2002031
version: 1
top level: BBa_S04186
location: https://synbiohub.org/public/igem/BBa_S04186/annotation2002031/range2002031/1
component: BBa_R0184

https://synbiohub.org/public/igem/BBa_S04186/annotation2002031/range2002031/1
type: Range
persistent identity: range2002031
display id: range2002031
version: 1
top level: BBa_S04186
direction: direction/reverse
start: 2890
end: 2933
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2002033
display id: annotation2002033
version: 1
top level: BBa_S04186
location: https://synbiohub.org/public/igem/BBa_S04186/annotation2002033/range2002033/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_S04186/annotation2002033/range2002033/1
type: Range
persistent identity: range2002033
display id: range2002033
version: 1
top level: BBa_S04186
direction: direction/forward
start: 2942
end: 2954
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2002036
display id: annotation2002036
version: 1
top level: BBa_S04186
location: https://synbiohub.org/public/igem/BBa_S04186/annotation2002036/range2002036/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_S04186/annotation2002036/range2002036/1
type: Range
persistent identity: range2002036
display id: range2002036
version: 1
top level: BBa_S04186
direction: direction/reverse
start: 2961
end: 3680
orientation: inline

BBa_B0016
type: SequenceAnnotation
persistent identity: annotation2002037
display id: annotation2002037
version: 1
top level: BBa_S04186
location: https://synbiohub.org/public/igem/BBa_S04186/annotation2002037/range2002037/1
component: BBa_B0016

https://synbiohub.org/public/igem/BBa_S04186/annotation2002037/range2002037/1
type: Range
persistent identity: range2002037
display id: range2002037
version: 1
top level: BBa_S04186
direction: direction/reverse
start: 3689
end: 3736
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_R0184
type: ComponentDefinition
persistent identity: BBa R0184
display id: BBa_R0184
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter (lacI repressible)
created: 2007-04-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -16.
owned by: james
owned by: myers
mutable description: A mutant version of the T7lac promoter designed by Dubendorff and Studier. The promoter is based on the strong ??10 T7 promoter but incorporates an A->C mutation at -16. The LacI operator site is a partially symmetric 25bp sequence.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The -16 mutation was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0183 fragment
sequence annotation: lacI binding site
sequence annotation: Center of binding site symmetry
sequence: https://synbiohub.org/public/igem/BBa_R0184_sequence/1

BBa_R0183 fragment
type: SequenceAnnotation
persistent identity: annotation1932678
display id: annotation1932678
version: 1
top level: BBa_R0184
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932678/range1932678/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0184/annotation1932678/range1932678/1
type: Range
persistent identity: range1932678
display id: range1932678
version: 1
top level: BBa_R0184
direction: direction/forward
start: 1
end: 20
orientation: inline

lacI binding site
type: SequenceAnnotation
persistent identity: annotation1932679
display id: annotation1932679
version: 1
top level: BBa_R0184
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932679/range1932679/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0184/annotation1932679/range1932679/1
type: Range
persistent identity: range1932679
display id: range1932679
version: 1
top level: BBa_R0184
direction: direction/forward
start: 20
end: 44
orientation: inline

Center of binding site symmetry
type: SequenceAnnotation
persistent identity: annotation1932680
display id: annotation1932680
version: 1
top level: BBa_R0184
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932680/range1932680/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_R0184/annotation1932680/range1932680/1
type: Range
persistent identity: range1932680
display id: range1932680
version: 1
top level: BBa_R0184
direction: direction/forward
start: 31
end: 31
orientation: inline

BBa_I2032
type: ComponentDefinition
persistent identity: BBa I2032
display id: BBa_I2032
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNA polymerase
created: 2007-04-11T11:00:00Z
modified: 2016-10-14T06:52:19Z
mutable provenance: Bacteriophage T7
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Gene 1 coding region from bacteriophage T7 encoding a RNAP polymerase (RNAP). This single-protein RNAP recognizes a 21 bp consensus promoter sequence and is orthogonal to E. coli RNAP.
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 27399
owner id: 135
owning group id: 84
sample status: In stock
status: status/Available
bookmark: false
mutable notes: n/a
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: putative E. coli promoter
sequence: https://synbiohub.org/public/igem/BBa_I2032_sequence/1

putative E. coli promoter
type: SequenceAnnotation
persistent identity: annotation1928674
display id: annotation1928674
version: 1
top level: BBa_I2032
location: https://synbiohub.org/public/igem/BBa_I2032/annotation1928674/range1928674/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I2032/annotation1928674/range1928674/1
type: Range
persistent identity: range1928674
display id: range1928674
version: 1
top level: BBa_I2032
direction: direction/forward
start: 2498
end: 2525
orientation: inline

BBa_B0016
type: ComponentDefinition
persistent identity: BBa B0016
display id: BBa_B0016
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (T7 RNAP specific, T_Phi)
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Sri Kosuri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: T7 gene 1 specific transcriptional terminator
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0016_sequence/1

https://synbiohub.org/public/igem/BBa_S04186_sequence/1
type: Sequence
persistent identity: BBa S04186 sequence
display id: BBa_S04186_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgata…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0184_sequence/1
type: Sequence
persistent identity: BBa R0184 sequence
display id: BBa_R0184_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcatacgactcactataggggaattgtgagcggataacaattcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I2032_sequence/1
type: Sequence
persistent identity: BBa I2032 sequence
display id: BBa_I2032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgtt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0016_sequence/1
type: Sequence
persistent identity: BBa B0016 sequence
display id: BBa_B0016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctagcataaccccttggggcctctaaacgggtcttgaggggttttttg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z