LOCUS       BBa_S04187               914 bp    DNA     linear       22-Dec-2024
DEFINITION  R2000:I13507
ACCESSION   BBa_S04187
VERSION     BBa_S04187.1  
FEATURES             Location/Qualifiers
     promoter        10..15
                     /label=-35
     promoter        33..38
                     /label=-10
     misc_feature    20..33
                     /label=Zif23
     misc_feature    1..9
                     /label=consensus
     misc_feature    45..45
                     /label=(+1)
     promoter        1..45
                     /label=BBa_R2000
     misc_feature    58..61
                     /label=conserved
     RBS             54..65
                     /label=BBa_B0034
     CDS             72..746
                     /label=mrfp1
     misc_feature    753..777
                     /label=Help:Barcodes
     CDS             72..752
                     /label=BBa_E1010
     stem_loop       797..840
                     /label=stem_loop
     misc_feature    786..865
                     /label=BBa_B0010
     terminator      786..865
                     /label=BBa_B0010
     stem_loop       881..900
                     /label=T7 TE
     misc_feature    907..907
                     /label=stop
     polyA_site      901..914
                     /label=polya
     misc_feature    874..914
                     /label=BBa_B0012
     terminator      874..914
                     /label=BBa_B0012
     misc_feature    54..914
                     /label=BBa_I13507
ORIGIN
        1 agtttattct tgacatggtc ccacgcgcgt gggatactac gtcagtacta gagaaagagg
       61 agaaatacta gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc
      121 gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc
      181 cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg
      241 cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg
      301 ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta
      361 tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg
      421 agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc
      481 agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc
      541 tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag
      601 ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca
      661 tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg
      721 ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac
      781 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      841 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
      901 tttctgcgtt tata
//