LOCUS       BBa_S04189              2015 bp    DNA     linear       10-Dec-2025
DEFINITION  J04450:I7101
ACCESSION   BBa_S04189
VERSION     BBa_S04189.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..901
                     /label=mrfp1
     misc_feature    908..932
                     /label=Help:Barcodes
     CDS             227..907
                     /label=BBa_E1010
     stem_loop       952..995
                     /label=stem_loop
     misc_feature    941..1020
                     /label=BBa_B0010
     terminator      941..1020
                     /label=BBa_B0010
     stem_loop       1036..1055
                     /label=T7 TE
     misc_feature    1062..1062
                     /label=stop
     polyA_site      1056..1069
                     /label=polya
     misc_feature    1029..1069
                     /label=BBa_B0012
     terminator      1029..1069
                     /label=BBa_B0012
     misc_feature    1..1069
                     /label=BBa_J04450
     misc_feature    1078..1096
                     /label=TetR 1
     misc_feature    1078..1131
                     /label=BBa_R0040
     promoter        1097..1102
                     /label=-35
     misc_feature    1103..1121
                     /label=TetR 2
     promoter        1120..1125
                     /label=-10
     promoter        1078..1131
                     /label=BBa_R0040
     RBS             1140..1152
                     /label=RBS-3\Weak
     RBS             1146..1149
                     /label=RBS
     misc_feature    1140..1152
                     /label=BBa_B0032
     RBS             1140..1152
                     /label=BBa_B0032
     CDS             1159..1878
                     /label=GFP protein
     CDS             1159..1878
                     /label=BBa_E0040
     stem_loop       1898..1941
                     /label=stem_loop
     misc_feature    1887..1966
                     /label=BBa_B0010
     terminator      1887..1966
                     /label=BBa_B0010
     stem_loop       1982..2001
                     /label=T7 TE
     misc_feature    2008..2008
                     /label=stop
     polyA_site      2002..2015
                     /label=polya
     misc_feature    1975..2015
                     /label=BBa_B0012
     terminator      1975..2015
                     /label=BBa_B0012
     misc_feature    1078..2015
                     /label=BBa_I7101
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga
      961 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1021 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagtcc
     1081 ctatcagtga tagagattga catccctatc agtgatagag atactgagca ctactagagt
     1141 cacacaggaa agtactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
     1201 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
     1261 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
     1321 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
     1381 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
     1441 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
     1501 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
     1561 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
     1621 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
     1681 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
     1741 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
     1801 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
     1861 gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     1921 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1981 tggctcacct tcgggtgggc ctttctgcgt ttata
//