##gff-version 3
##sequence-region BBa_S04189 1 2015
BBa_S04189	.	engineered_region	1	1069	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_S04189	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_S04189	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04189	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_S04189	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_S04189	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_S04189	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_S04189	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_S04189	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_S04189	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_S04189	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_S04189	.	CDS	227	907	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_S04189	.	CDS	227	901	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_S04189	.	engineered_tag	908	932	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_S04189	.	terminator	941	1020	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_S04189	.	stem_loop	952	995	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04189	.	engineered_region	941	1020	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04189	.	terminator	1029	1069	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_S04189	.	stem_loop	1036	1055	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04189	.	polyA_site	1056	1069	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04189	.	stop_codon	1062	1062	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04189	.	engineered_region	1029	1069	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04189	.	engineered_region	1078	2015	.	+	0	ID=BBa_I7101;Name=BBa_I7101
BBa_S04189	.	CDS	1159	1878	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I7101
BBa_S04189	.	CDS	1159	1878	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_S04189	.	terminator	1975	2015	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I7101
BBa_S04189	.	stem_loop	1982	2001	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04189	.	polyA_site	2002	2015	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04189	.	stop_codon	2008	2008	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04189	.	engineered_region	1975	2015	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04189	.	terminator	1887	1966	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I7101
BBa_S04189	.	stem_loop	1898	1941	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04189	.	engineered_region	1887	1966	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04189	.	ribosome_entry_site	1140	1152	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_I7101
BBa_S04189	.	engineered_region	1140	1152	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04189	.	ribosome_entry_site	1146	1149	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04189	.	ribosome_entry_site	1140	1152	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04189	.	promoter	1078	1131	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I7101
BBa_S04189	.	engineered_region	1078	1131	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S04189	.	promoter	1097	1102	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S04189	.	non_covalent_binding_site	1103	1121	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S04189	.	promoter	1120	1125	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S04189	.	non_covalent_binding_site	1078	1096	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
>BBa_S04189
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctc
cgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaa
ggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcg
cttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacct
gaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag
gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggtta
tgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaa
aatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcag
ctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacg
aacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccc
tatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcact
ggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtg
atgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcac
tactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatg
cccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttg
aaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaatt
ggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatt
agacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcc
ttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtcct
tcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttata