LOCUS       BBa_S04190              1533 bp    DNA     linear       16-Dec-2024
DEFINITION  I6413:I7101
ACCESSION   BBa_S04190
VERSION     BBa_S04190.1  
FEATURES             Location/Qualifiers
     promoter        10..15
                     /label=-35
     promoter        33..38
                     /label=-10
     misc_feature    20..33
                     /label=Zif23
     misc_feature    1..9
                     /label=consensus
     misc_feature    45..45
                     /label=(+1)
     promoter        1..45
                     /label=BBa_R2000
     misc_feature    58..61
                     /label=conserved
     RBS             54..65
                     /label=BBa_B0034
     misc_feature    72..86
                     /label=lacZ gene fragment
     CDS             72..425
                     /label=lacZ alpha
     promoter        97..106
                     /label=T3 promoter
     promoter        244..262
                     /label=T7 promoter
     misc_feature    149..149
                     /label=C
     misc_feature    152..152
                     /label=T
     misc_feature    158..158
                     /label=A
     misc_feature    179..179
                     /label=C
     misc_feature    182..182
                     /label=G
     misc_feature    270..419
                     /label=lacZ gene fragment
     misc_feature    419..419
                     /label=inserted T
     misc_feature    426..450
                     /label=barcode
     misc_feature    72..450
                     /label=BBa_E0035
     stem_loop       470..513
                     /label=stem_loop
     misc_feature    459..538
                     /label=BBa_B0010
     terminator      459..538
                     /label=BBa_B0010
     stem_loop       554..573
                     /label=T7 TE
     misc_feature    580..580
                     /label=stop
     polyA_site      574..587
                     /label=polya
     misc_feature    547..587
                     /label=BBa_B0012
     terminator      547..587
                     /label=BBa_B0012
     terminator      459..587
                     /label=BBa_B0015
     misc_feature    54..587
                     /label=BBa_E0435
     misc_feature    1..587
                     /label=BBa_I6413
     misc_feature    596..614
                     /label=TetR 1
     misc_feature    596..649
                     /label=BBa_R0040
     promoter        615..620
                     /label=-35
     misc_feature    621..639
                     /label=TetR 2
     promoter        638..643
                     /label=-10
     promoter        596..649
                     /label=BBa_R0040
     RBS             658..670
                     /label=RBS-3\Weak
     RBS             664..667
                     /label=RBS
     misc_feature    658..670
                     /label=BBa_B0032
     RBS             658..670
                     /label=BBa_B0032
     CDS             677..1396
                     /label=GFP protein
     CDS             677..1396
                     /label=BBa_E0040
     stem_loop       1416..1459
                     /label=stem_loop
     misc_feature    1405..1484
                     /label=BBa_B0010
     terminator      1405..1484
                     /label=BBa_B0010
     stem_loop       1500..1519
                     /label=T7 TE
     misc_feature    1526..1526
                     /label=stop
     polyA_site      1520..1533
                     /label=polya
     misc_feature    1493..1533
                     /label=BBa_B0012
     terminator      1493..1533
                     /label=BBa_B0012
     misc_feature    596..1533
                     /label=BBa_I7101
ORIGIN
        1 agtttattct tgacatggtc ccacgcgcgt gggatactac gtcagtacta gagaaagagg
       61 agaaatacta gatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
      121 aaagctggag ctccaccgcg gtggcggcag cactagagct agtggatccc ccgggctgta
      181 gaaattcgat atcaagctta tcgataccgt cgacctcgag ggggggcccg gtacccaatt
      241 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
      301 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      361 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatt
      421 aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa
      481 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      541 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtccct
      601 atcagtgata gagattgaca tccctatcag tgatagagat actgagcact actagagtca
      661 cacaggaaag tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct
      721 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
      781 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
      841 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc
      901 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
      961 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga
     1021 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
     1081 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
     1141 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag
     1201 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     1261 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
     1321 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
     1381 actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1441 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1501 gctcaccttc gggtgggcct ttctgcgttt ata
//