BBa_S04190
type: ComponentDefinition
persistent identity: BBa S04190
display id: BBa_S04190
version: 1
was generated by: iGEM to SBOL conversion
description: I6413:I7101
created: 2009-03-25T12:00:00Z
modified: 2015-05-08T01:14:36Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _84_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Bartholomew Canton
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_I7101
component: BBa_I6413
sequence annotation: BBa_I6413
sequence annotation: BBa_I7101
sequence: https://synbiohub.org/public/igem/BBa_S04190_sequence/1

BBa_I7101
type: Component
persistent identity: component2283401
display id: component2283401
version: 1
top level: BBa_S04190
definition: BBa_I7101
access: public

BBa_I6413
type: Component
persistent identity: component2283385
display id: component2283385
version: 1
top level: BBa_S04190
definition: BBa_I6413
access: public

BBa_I6413
type: SequenceAnnotation
persistent identity: annotation2283385
display id: annotation2283385
version: 1
top level: BBa_S04190
location: https://synbiohub.org/public/igem/BBa_S04190/annotation2283385/range2283385/1
component: BBa_I6413

https://synbiohub.org/public/igem/BBa_S04190/annotation2283385/range2283385/1
type: Range
persistent identity: range2283385
display id: range2283385
version: 1
top level: BBa_S04190
direction: direction/reverse
start: 1
end: 587
orientation: inline

BBa_I7101
type: SequenceAnnotation
persistent identity: annotation2283401
display id: annotation2283401
version: 1
top level: BBa_S04190
location: https://synbiohub.org/public/igem/BBa_S04190/annotation2283401/range2283401/1
component: BBa_I7101

https://synbiohub.org/public/igem/BBa_S04190/annotation2283401/range2283401/1
type: Range
persistent identity: range2283401
display id: range2283401
version: 1
top level: BBa_S04190
direction: direction/reverse
start: 596
end: 1533
orientation: inline

BBa_I6413
type: ComponentDefinition
persistent identity: BBa I6413
display id: BBa_I6413
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter high output protein generator
created: 2008-01-09T12:00:00Z
modified: 2015-08-31T04:07:44Z
mutable provenance: This is a composite part.
owned by: james
owned by: myers
mutable description: Produces lacZalpha constitutively in the absence of BBa_C2006 repressor.
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Measurement
role: SO:0000804
component: BBa_R2000
component: BBa_E0435
sequence annotation: BBa_R2000
sequence annotation: BBa_E0435
sequence: https://synbiohub.org/public/igem/BBa_I6413_sequence/1

BBa_R2000
type: Component
persistent identity: component2253498
display id: component2253498
version: 1
top level: BBa_I6413
definition: Zif23
access: public

BBa_E0435
type: Component
persistent identity: component2253521
display id: component2253521
version: 1
top level: BBa_I6413
definition: BBa_E0435
access: public

BBa_R2000
type: SequenceAnnotation
persistent identity: annotation2253498
display id: annotation2253498
version: 1
top level: BBa_I6413
location: https://synbiohub.org/public/igem/BBa_I6413/annotation2253498/range2253498/1
component: BBa_R2000

https://synbiohub.org/public/igem/BBa_I6413/annotation2253498/range2253498/1
type: Range
persistent identity: range2253498
display id: range2253498
version: 1
top level: BBa_I6413
direction: direction/reverse
start: 1
end: 45
orientation: inline

BBa_E0435
type: SequenceAnnotation
persistent identity: annotation2253521
display id: annotation2253521
version: 1
top level: BBa_I6413
location: https://synbiohub.org/public/igem/BBa_I6413/annotation2253521/range2253521/1
component: BBa_E0435

https://synbiohub.org/public/igem/BBa_I6413/annotation2253521/range2253521/1
type: Range
persistent identity: range2253521
display id: range2253521
version: 1
top level: BBa_I6413
direction: direction/reverse
start: 54
end: 587
orientation: inline

BBa_I7101
type: ComponentDefinition
persistent identity: BBa I7101
display id: BBa_I7101
version: 1
was generated by: iGEM to SBOL conversion
description: R0040.B0032.E0040.B0015
created: 2004-10-25T11:00:00Z
modified: 2015-08-31T04:07:45Z
owned by: james
owned by: myers
mutable description: R0040.B0032.E0040.B0015, GFP expression device
discontinued: false
dominant: true
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: jcbraff
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_B0010
component: BBa_E0040
component: BBa_B0032
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I7101_sequence/1

BBa_B0012
type: Component
persistent identity: component1683641
display id: component1683641
version: 1
top level: BBa_I7101
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1683631
display id: component1683631
version: 1
top level: BBa_I7101
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1683626
display id: component1683626
version: 1
top level: BBa_I7101
definition: GFP
access: public

BBa_B0032
type: Component
persistent identity: component1683623
display id: component1683623
version: 1
top level: BBa_I7101
definition: BBa_B0032
access: public

BBa_R0040
type: Component
persistent identity: component1683612
display id: component1683612
version: 1
top level: BBa_I7101
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1683612
display id: annotation1683612
version: 1
top level: BBa_I7101
location: https://synbiohub.org/public/igem/BBa_I7101/annotation1683612/range1683612/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I7101/annotation1683612/range1683612/1
type: Range
persistent identity: range1683612
display id: range1683612
version: 1
top level: BBa_I7101
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1683623
display id: annotation1683623
version: 1
top level: BBa_I7101
location: https://synbiohub.org/public/igem/BBa_I7101/annotation1683623/range1683623/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I7101/annotation1683623/range1683623/1
type: Range
persistent identity: range1683623
display id: range1683623
version: 1
top level: BBa_I7101
direction: direction/forward
start: 63
end: 75
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1683626
display id: annotation1683626
version: 1
top level: BBa_I7101
location: https://synbiohub.org/public/igem/BBa_I7101/annotation1683626/range1683626/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I7101/annotation1683626/range1683626/1
type: Range
persistent identity: range1683626
display id: range1683626
version: 1
top level: BBa_I7101
direction: direction/forward
start: 82
end: 801
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1683631
display id: annotation1683631
version: 1
top level: BBa_I7101
location: https://synbiohub.org/public/igem/BBa_I7101/annotation1683631/range1683631/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I7101/annotation1683631/range1683631/1
type: Range
persistent identity: range1683631
display id: range1683631
version: 1
top level: BBa_I7101
direction: direction/forward
start: 810
end: 889
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1683641
display id: annotation1683641
version: 1
top level: BBa_I7101
location: https://synbiohub.org/public/igem/BBa_I7101/annotation1683641/range1683641/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I7101/annotation1683641/range1683641/1
type: Range
persistent identity: range1683641
display id: range1683641
version: 1
top level: BBa_I7101
direction: direction/forward
start: 898
end: 938
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_E0435
type: ComponentDefinition
persistent identity: BBa E0435
display id: BBa_E0435
version: 1
was generated by: iGEM to SBOL conversion
description: PoPS->lacZalpha reporter device
created: 2007-12-21T12:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: It is derived from BBa_E0433.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This device takes a PoPS signal in and produces the protein lacZalpha. When put in a strain that can provide alpha-complementation, you should see measurable beta-galactosidase activity.
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An additional T was inserted to ensure that the double stop codon is in frame with the rest of the protein.
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_E0035
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_E0035
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_E0435_sequence/1

BBa_B0015
type: Component
persistent identity: component2249043
display id: component2249043
version: 1
top level: BBa_E0435
definition: BBa_B0015
access: public

BBa_E0035
type: Component
persistent identity: component2249036
display id: component2249036
version: 1
top level: BBa_E0435
definition: BBa_E0035
access: public

BBa_B0034
type: Component
persistent identity: component2249023
display id: component2249023
version: 1
top level: BBa_E0435
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2249023
display id: annotation2249023
version: 1
top level: BBa_E0435
location: https://synbiohub.org/public/igem/BBa_E0435/annotation2249023/range2249023/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0435/annotation2249023/range2249023/1
type: Range
persistent identity: range2249023
display id: range2249023
version: 1
top level: BBa_E0435
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E0035
type: SequenceAnnotation
persistent identity: annotation2249036
display id: annotation2249036
version: 1
top level: BBa_E0435
location: https://synbiohub.org/public/igem/BBa_E0435/annotation2249036/range2249036/1
component: BBa_E0035

https://synbiohub.org/public/igem/BBa_E0435/annotation2249036/range2249036/1
type: Range
persistent identity: range2249036
display id: range2249036
version: 1
top level: BBa_E0435
direction: direction/reverse
start: 19
end: 397
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2249043
display id: annotation2249043
version: 1
top level: BBa_E0435
location: https://synbiohub.org/public/igem/BBa_E0435/annotation2249043/range2249043/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_E0435/annotation2249043/range2249043/1
type: Range
persistent identity: range2249043
display id: range2249043
version: 1
top level: BBa_E0435
direction: direction/reverse
start: 406
end: 534
orientation: inline

Zif23
type: ComponentDefinition
persistent identity: BBa R2000
display id: BBa_R2000
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter, Zif23 regulated, test: between
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: synthetic
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter with standard -10 and -35 sites. A symmetric Zif23 dimer target site is between the -10 and -35 sites.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: consensus sequences based on [Jensen98].
star: true
creator: Ronny Krashinsky
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: consensus
sequence annotation: -35
sequence annotation: Zif23
sequence annotation: -10
sequence annotation: (+1)
sequence: https://synbiohub.org/public/igem/BBa_R2000_sequence/1

consensus
type: SequenceAnnotation
persistent identity: annotation318100
display id: annotation318100
version: 1
top level: Zif23
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318100/range318100/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R2000/annotation318100/range318100/1
type: Range
persistent identity: range318100
display id: range318100
version: 1
top level: Zif23
direction: direction/forward
start: 1
end: 9
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation318094
display id: annotation318094
version: 1
top level: Zif23
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318094/range318094/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R2000/annotation318094/range318094/1
type: Range
persistent identity: range318094
display id: range318094
version: 1
top level: Zif23
direction: direction/forward
start: 10
end: 15
orientation: inline

Zif23
type: SequenceAnnotation
persistent identity: annotation318099
display id: annotation318099
version: 1
top level: Zif23
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318099/range318099/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R2000/annotation318099/range318099/1
type: Range
persistent identity: range318099
display id: range318099
version: 1
top level: Zif23
direction: direction/forward
start: 20
end: 33
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318098
display id: annotation318098
version: 1
top level: Zif23
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318098/range318098/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R2000/annotation318098/range318098/1
type: Range
persistent identity: range318098
display id: range318098
version: 1
top level: Zif23
direction: direction/forward
start: 33
end: 38
orientation: inline

(+1)
type: SequenceAnnotation
persistent identity: annotation318102
display id: annotation318102
version: 1
top level: Zif23
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318102/range318102/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R2000/annotation318102/range318102/1
type: Range
persistent identity: range318102
display id: range318102
version: 1
top level: Zif23
direction: direction/forward
start: 45
end: 45
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_E0035
type: ComponentDefinition
persistent identity: BBa E0035
display id: BBa_E0035
version: 1
was generated by: iGEM to SBOL conversion
description: LacZ alpha fragment; complements matching N-terminal deletion mutant (lacZ-omega)
created: 2007-12-21T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: This part is derived from BBa_E0033.
owned by: james
owned by: myers
mutable description: This part encodes the alpha fragment of the lacZ gene. To greatly reduce the number of bases, this is only a portion of the LacZ gene. It can be used as a detection system with N-terminal deletion mutants of lacZ. Combination of lacZ-alpha with such a deletion mutant protein restores enzyme activity that can be assayed colorimetrically or more sensitively with chemiluminescent substrates.
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: It has the additional T needed to ensure that the double stop codon is in frame.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: lacZ gene fragment
sequence annotation: lacZ alpha
sequence annotation: T3 promoter
sequence annotation: C
sequence annotation: T
sequence annotation: A
sequence annotation: G
sequence annotation: T7 promoter
sequence annotation: inserted T
sequence annotation: barcode
sequence: https://synbiohub.org/public/igem/BBa_E0035_sequence/1

lacZ gene fragment
type: SequenceAnnotation
persistent identity: annotation1959035
display id: annotation1959035
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959035/range1959035/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_E0035/annotation1959035/range1959035/1
type: Range
persistent identity: range1959035
display id: range1959035
version: 1
top level: BBa_E0035
direction: direction/forward
start: 1
end: 15
orientation: inline

lacZ alpha
type: SequenceAnnotation
persistent identity: annotation1959036
display id: annotation1959036
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959036/range1959036/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0035/annotation1959036/range1959036/1
type: Range
persistent identity: range1959036
display id: range1959036
version: 1
top level: BBa_E0035
direction: direction/forward
start: 1
end: 354
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1959037
display id: annotation1959037
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959037/range1959037/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_E0035/annotation1959037/range1959037/1
type: Range
persistent identity: range1959037
display id: range1959037
version: 1
top level: BBa_E0035
direction: direction/forward
start: 26
end: 35
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation1959039
display id: annotation1959039
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959039/range1959039/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0035/annotation1959039/range1959039/1
type: Range
persistent identity: range1959039
display id: range1959039
version: 1
top level: BBa_E0035
direction: direction/forward
start: 78
end: 78
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1959040
display id: annotation1959040
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959040/range1959040/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0035/annotation1959040/range1959040/1
type: Range
persistent identity: range1959040
display id: range1959040
version: 1
top level: BBa_E0035
direction: direction/forward
start: 81
end: 81
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1959041
display id: annotation1959041
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959041/range1959041/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0035/annotation1959041/range1959041/1
type: Range
persistent identity: range1959041
display id: range1959041
version: 1
top level: BBa_E0035
direction: direction/forward
start: 87
end: 87
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation1959042
display id: annotation1959042
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959042/range1959042/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_E0035/annotation1959042/range1959042/1
type: Range
persistent identity: range1959042
display id: range1959042
version: 1
top level: BBa_E0035
direction: direction/forward
start: 108
end: 108
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation1959043
display id: annotation1959043
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959043/range1959043/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_E0035/annotation1959043/range1959043/1
type: Range
persistent identity: range1959043
display id: range1959043
version: 1
top level: BBa_E0035
direction: direction/forward
start: 111
end: 111
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1959038
display id: annotation1959038
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959038/range1959038/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_E0035/annotation1959038/range1959038/1
type: Range
persistent identity: range1959038
display id: range1959038
version: 1
top level: BBa_E0035
direction: direction/forward
start: 173
end: 191
orientation: inline

lacZ gene fragment
type: SequenceAnnotation
persistent identity: annotation1959044
display id: annotation1959044
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959044/range1959044/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_E0035/annotation1959044/range1959044/1
type: Range
persistent identity: range1959044
display id: range1959044
version: 1
top level: BBa_E0035
direction: direction/forward
start: 199
end: 348
orientation: inline

inserted T
type: SequenceAnnotation
persistent identity: annotation1959045
display id: annotation1959045
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959045/range1959045/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_E0035/annotation1959045/range1959045/1
type: Range
persistent identity: range1959045
display id: range1959045
version: 1
top level: BBa_E0035
direction: direction/forward
start: 348
end: 348
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation1959046
display id: annotation1959046
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959046/range1959046/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_E0035/annotation1959046/range1959046/1
type: Range
persistent identity: range1959046
display id: range1959046
version: 1
top level: BBa_E0035
direction: direction/forward
start: 355
end: 379
orientation: inline

https://synbiohub.org/public/igem/BBa_S04190_sequence/1
type: Sequence
persistent identity: BBa S04190 sequence
display id: BBa_S04190_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtttattcttgacatggtcccacgcgcgtgggatactacgtcagtactagagaaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaattaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I6413_sequence/1
type: Sequence
persistent identity: BBa I6413 sequence
display id: BBa_I6413_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtttattcttgacatggtcccacgcgcgtgggatactacgtcagtactagagaaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaattaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I7101_sequence/1
type: Sequence
persistent identity: BBa I7101 sequence
display id: BBa_I7101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0435_sequence/1
type: Sequence
persistent identity: BBa E0435 sequence
display id: BBa_E0435_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaattaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R2000_sequence/1
type: Sequence
persistent identity: BBa R2000 sequence
display id: BBa_R2000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtttattcttgacatggtcccacgcgcgtgggatactacgtcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0035_sequence/1
type: Sequence
persistent identity: BBa E0035 sequence
display id: BBa_E0035_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaattaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z