##gff-version 3
##sequence-region BBa_S04191 1 1533
BBa_S04191	.	engineered_region	947	1533	.	+	0	ID=BBa_I6413;Name=BBa_I6413
BBa_S04191	.	engineered_region	1000	1533	.	+	0	ID=BBa_E0435;Name=BBa_E0435;Parent=BBa_I6413
BBa_S04191	.	ribosome_entry_site	1000	1011	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0435
BBa_S04191	.	conserved_region	1004	1007	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04191	.	terminator	1405	1533	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_E0435
BBa_S04191	.	terminator	1493	1533	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04191	.	polyA_site	1520	1533	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04191	.	engineered_region	1493	1533	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04191	.	stem_loop	1500	1519	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04191	.	stop_codon	1526	1526	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04191	.	terminator	1405	1484	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04191	.	stem_loop	1416	1459	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04191	.	engineered_region	1405	1484	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04191	.	engineered_region	1018	1396	.	+	0	ID=BBa_E0035;Name=BBa_E0035;Parent=BBa_E0435
BBa_S04191	.	sequence_alteration	1095	1095	.	+	0	ID=annotation1959039;Name=C;Parent=BBa_E0035
BBa_S04191	.	sequence_alteration	1098	1098	.	+	0	ID=annotation1959040;Name=T;Parent=BBa_E0035
BBa_S04191	.	sequence_alteration	1125	1125	.	+	0	ID=annotation1959042;Name=C;Parent=BBa_E0035
BBa_S04191	.	sequence_alteration	1104	1104	.	+	0	ID=annotation1959041;Name=A;Parent=BBa_E0035
BBa_S04191	.	sequence_feature	1018	1032	.	+	0	ID=annotation1959035;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_S04191	.	sequence_alteration	1365	1365	.	+	0	ID=annotation1959045;Name=inserted T;Parent=BBa_E0035
BBa_S04191	.	promoter	1043	1052	.	+	0	ID=annotation1959037;Name=T3 promoter;Parent=BBa_E0035
BBa_S04191	.	engineered_tag	1372	1396	.	+	0	ID=annotation1959046;Name=barcode;Parent=BBa_E0035
BBa_S04191	.	sequence_alteration	1128	1128	.	+	0	ID=annotation1959043;Name=G;Parent=BBa_E0035
BBa_S04191	.	CDS	1018	1371	.	+	0	ID=annotation1959036;Name=lacZ alpha;Parent=BBa_E0035
BBa_S04191	.	promoter	1190	1208	.	+	0	ID=annotation1959038;Name=T7 promoter;Parent=BBa_E0035
BBa_S04191	.	sequence_feature	1216	1365	.	+	0	ID=annotation1959044;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_S04191	.	promoter	947	991	.	+	0	ID=BBa_R2000;Name=BBa_R2000;Parent=BBa_I6413
BBa_S04191	.	start_codon	991	991	.	+	0	ID=annotation318102;Name=(+1);Parent=BBa_R2000
BBa_S04191	.	non_covalent_binding_site	966	979	.	+	0	ID=annotation318099;Name=Zif23;Parent=BBa_R2000
BBa_S04191	.	sequence_feature	947	955	.	+	0	ID=annotation318100;Name=consensus;Parent=BBa_R2000
BBa_S04191	.	promoter	979	984	.	+	0	ID=annotation318098;Name=-10;Parent=BBa_R2000
BBa_S04191	.	promoter	956	961	.	+	0	ID=annotation318094;Name=-35;Parent=BBa_R2000
BBa_S04191	.	engineered_region	1	938	.	+	0	ID=BBa_I7101;Name=BBa_I7101
BBa_S04191	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_I7101
BBa_S04191	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04191	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04191	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04191	.	terminator	898	938	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I7101
BBa_S04191	.	polyA_site	925	938	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04191	.	engineered_region	898	938	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04191	.	stem_loop	905	924	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04191	.	stop_codon	931	931	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04191	.	terminator	810	889	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I7101
BBa_S04191	.	stem_loop	821	864	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04191	.	engineered_region	810	889	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04191	.	CDS	82	801	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I7101
BBa_S04191	.	CDS	82	801	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_S04191	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I7101
BBa_S04191	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S04191	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S04191	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S04191	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S04191	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
>BBa_S04191
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtact
agatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaa
attttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaa
ctacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatga
aacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaa
ctacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaa
gaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaa
agaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaaca
aaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagat
cccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactat
acaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagag
tttattcttgacatggtcccacgcgcgtgggatactacgtcagtactagagaaagaggagaaatactagatgaccatga
ttacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagct
agtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaat
tcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgtta
cccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttc
ccaacagttgcgcagcctgaattaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttata