LOCUS       BBa_S04206              2358 bp    DNA     linear       16-Jun-2024
DEFINITION  R0010:C0179:I0462
ACCESSION   BBa_S04206
VERSION     BBa_S04206.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1151..1189
                     /label=LVA
     CDS             62..1189
                     /label=lacI-LVA
     misc_feature    62..1189
                     /label=BBa_C0012
     misc_feature    1190..1214
                     /label=Help:Barcodes
     CDS             62..1189
                     /label=BBa_C0012
     stem_loop       1234..1277
                     /label=stem_loop
     misc_feature    1223..1302
                     /label=BBa_B0010
     terminator      1223..1302
                     /label=BBa_B0010
     stem_loop       1318..1337
                     /label=T7 TE
     misc_feature    1344..1344
                     /label=stop
     polyA_site      1338..1351
                     /label=polya
     misc_feature    1311..1351
                     /label=BBa_B0012
     terminator      1311..1351
                     /label=BBa_B0012
     terminator      1223..1351
                     /label=BBa_B0015
     misc_feature    1362..1378
                     /label=lac O1
     promoter        1379..1384
                     /label=-35
     misc_feature    1385..1401
                     /label=lac O1
     promoter        1402..1407
                     /label=-10
     misc_feature    1360..1413
                     /label=BBa_R0011
     promoter        1360..1413
                     /label=BBa_R0011
     misc_feature    44..1414
                     /label=BBa_Q04121
     misc_feature    1427..1430
                     /label=conserved
     RBS             1423..1434
                     /label=BBa_B0034
     misc_feature    44..1434
                     /label=BBa_S04302
     CDS             1441..2157
                     /label=lasR
     misc_feature    2158..2190
                     /label=LVA
     misc_feature    1920..1920
                     /label=G
     misc_feature    2197..2221
                     /label=Help:Barcodes
     CDS             1441..2221
                     /label=BBa_C0079
     stem_loop       2241..2284
                     /label=stem_loop
     misc_feature    2230..2309
                     /label=BBa_B0010
     terminator      2230..2309
                     /label=BBa_B0010
     stem_loop       2325..2344
                     /label=T7 TE
     misc_feature    2351..2351
                     /label=stop
     polyA_site      2345..2358
                     /label=polya
     misc_feature    2318..2358
                     /label=BBa_B0012
     terminator      2318..2358
                     /label=BBa_B0012
     terminator      2230..2358
                     /label=BBa_B0015
     CDS             1441..2358
                     /label=BBa_S04301
     misc_feature    44..2358
                     /label=BBa_S04303
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
      121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
      181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
      241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
      301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
      361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
      421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
      481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
      541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
      601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
      661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
      721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
      781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
      841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
      901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
      961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg
     1201 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1321 tcaccttcgg gtgggccttt ctgcgtttat atactagaga attgtgagcg gataacaatt
     1381 gacattgtga gcggataaca agatactgag cacatactag agaaagagga gaaatactag
     1441 atggccttgg ttgacggttt tcttgagctg gaacgctcaa gtggaaaatt ggagtggagc
     1501 gccatcctcc agaagatggc gagcgacctt ggattctcga agatcctgtt cggcctgttg
     1561 cctaaggaca gccaggacta cgagaacgcc ttcatcgtcg gcaactaccc ggccgcctgg
     1621 cgcgagcatt acgaccgggc tggctacgcg cgggtcgacc cgacggtcag tcactgtacc
     1681 cagagcgtac tgccgatttt ctgggaaccg tccatctacc agacgcgaaa gcagcacgag
     1741 ttcttcgagg aagcctcggc cgccggcctg gtgtatgggc tgaccatgcc gctgcatggt
     1801 gctcgcggcg aactcggcgc gctgagcctc agcgtggaag cggaaaaccg ggccgaggcc
     1861 aaccgtttca tagagtcggt cctgccgacc ctgtggatgc tcaaggacta cgcactgcaa
     1921 agcggtgccg gactggcctt cgaacatccg gtcagcaaac cggtggttct gaccagccgg
     1981 gagaaggaag tgttgcagtg gtgcgccatc ggcaagacca gttgggagat atcggttatc
     2041 tgcaactgct cggaagccaa tgtgaacttc catatgggaa atattcggcg gaagttcggt
     2101 gtgacctccc gccgcgtagc ggccattatg gccgttaatt tgggtcttat tactctcgct
     2161 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca
     2221 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2281 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     2341 ggcctttctg cgtttata
//