LOCUS BBa_S04210 4256 bp DNA linear 05-Nov-2024 DEFINITION J23100:J06800:B0034:C0079:B0015 ACCESSION BBa_S04210 VERSION BBa_S04210.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23116 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 65..681 /label=tetR misc_feature 682..715 /label=SsrA misc_feature 722..746 /label=Help:Barcodes CDS 62..746 /label=BBa_C0040 stem_loop 766..809 /label=stem_loop misc_feature 755..834 /label=BBa_B0010 terminator 755..834 /label=BBa_B0010 stem_loop 850..869 /label=T7 TE misc_feature 876..876 /label=stop polyA_site 870..883 /label=polya misc_feature 843..883 /label=BBa_B0012 terminator 843..883 /label=BBa_B0012 terminator 755..883 /label=BBa_B0015 misc_feature 1..883 /label=BBa_K145201 misc_feature 892..910 /label=TetR 1 misc_feature 892..945 /label=BBa_R0040 promoter 911..916 /label=-35 misc_feature 917..935 /label=TetR 2 promoter 934..939 /label=-10 promoter 892..945 /label=BBa_R0040 RBS 954..966 /label=RBS-3\Weak RBS 960..963 /label=RBS misc_feature 954..966 /label=BBa_B0032 RBS 954..966 /label=BBa_B0032 misc_feature 973..974 /label=prefix misc_feature 1146..1146 /label=T misc_feature 1464..1464 /label=A CDS 973..1722 /label=luxR misc_feature 973..1728 /label=BBa_C0062 misc_feature 1729..1753 /label=Help:Barcodes CDS 973..1728 /label=BBa_C0062 misc_feature 954..1753 /label=BBa_K116616 stem_loop 1773..1816 /label=stem_loop misc_feature 1762..1841 /label=BBa_B0010 terminator 1762..1841 /label=BBa_B0010 stem_loop 1857..1876 /label=T7 TE misc_feature 1883..1883 /label=stop polyA_site 1877..1890 /label=polya misc_feature 1850..1890 /label=BBa_B0012 terminator 1850..1890 /label=BBa_B0012 terminator 1762..1890 /label=BBa_B0015 misc_feature 954..1890 /label=BBa_K116626 misc_feature 892..1890 /label=BBa_K116640 misc_feature 1..1890 /label=BBa_S04215 promoter 1899..1933 /label=BBa_J23100 misc_feature 1946..1949 /label=conserved RBS 1942..1953 /label=BBa_B0034 misc_feature 3049..3087 /label=LVA CDS 1960..3087 /label=lacI-LVA misc_feature 1960..3087 /label=BBa_C0012 misc_feature 3088..3112 /label=Help:Barcodes CDS 1960..3087 /label=BBa_C0012 stem_loop 3132..3175 /label=stem_loop misc_feature 3121..3200 /label=BBa_B0010 terminator 3121..3200 /label=BBa_B0010 stem_loop 3216..3235 /label=T7 TE misc_feature 3242..3242 /label=stop polyA_site 3236..3249 /label=polya misc_feature 3209..3249 /label=BBa_B0012 terminator 3209..3249 /label=BBa_B0012 terminator 3121..3249 /label=BBa_B0015 misc_feature 3260..3276 /label=lac O1 promoter 3277..3282 /label=-35 misc_feature 3283..3299 /label=lac O1 promoter 3300..3305 /label=-10 misc_feature 3258..3311 /label=BBa_R0011 promoter 3258..3311 /label=BBa_R0011 misc_feature 1942..3312 /label=BBa_Q04121 misc_feature 3325..3328 /label=conserved RBS 3321..3332 /label=BBa_B0034 misc_feature 1942..3332 /label=BBa_S04302 CDS 3339..4055 /label=lasR misc_feature 4056..4088 /label=LVA misc_feature 3818..3818 /label=G misc_feature 4095..4119 /label=Help:Barcodes CDS 3339..4119 /label=BBa_C0079 stem_loop 4139..4182 /label=stem_loop misc_feature 4128..4207 /label=BBa_B0010 terminator 4128..4207 /label=BBa_B0010 stem_loop 4223..4242 /label=T7 TE misc_feature 4249..4249 /label=stop polyA_site 4243..4256 /label=polya misc_feature 4216..4256 /label=BBa_B0012 terminator 4216..4256 /label=BBa_B0012 terminator 4128..4256 /label=BBa_B0015 CDS 3339..4256 /label=BBa_S04301 misc_feature 1942..4256 /label=BBa_S04303 misc_feature 1899..4256 /label=BBa_S04206 ORIGIN 1 ttgacagcta gctcagtcct agggactatg ctagctacta gagaaagagg agaaatacta 61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca 901 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagtcacaca 961 ggaaagtact agatgaaaaa cataaatgcc gacgacacat acagaataat taataaaatt 1021 aaagcttgta gaagcaataa tgatattaat caatgcttat ctgatatgac taaaatggta 1081 cattgtgaat attatttact cgcgatcatt tatcctcatt ctatggttaa atctgatatt 1141 tcaatcctag ataattaccc taaaaaatgg aggcaatatt atgatgacgc taatttaata 1201 aaatatgatc ctatagtaga ttattctaac tccaatcatt caccaattaa ttggaatata 1261 tttgaaaaca atgctgtaaa taaaaaatct ccaaatgtaa ttaaagaagc gaaaacatca 1321 ggtcttatca ctgggtttag tttccctatt catacggcta acaatggctt cggaatgctt 1381 agttttgcac attcagaaaa agacaactat atagatagtt tatttttaca tgcgtgtatg 1441 aacataccat taattgttcc ttctctagtt gataattatc gaaaaataaa tatagcaaat 1501 aataaatcaa acaacgattt aaccaaaaga gaaaaagaat gtttagcgtg ggcatgcgaa 1561 ggaaaaagct cttgggatat ttcaaaaata ttaggttgca gtgagcgtac tgtcactttc 1621 catttaacca atgcgcaaat gaaactcaat acaacaaacc gctgccaaag tatttctaaa 1681 gcaattttaa caggagcaat tgattgccca tactttaaaa attaataaca ctgatagtgc 1741 tagtgtagat cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 1801 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 1861 caccttcggg tgggcctttc tgcgtttata tactagagtt gacggctagc tcagtcctag 1921 gtacagtgct agctactaga gaaagaggag aaatactaga tggtgaatgt gaaaccagta 1981 acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg 2041 aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag 2101 ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt 2161 ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa 2221 tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc 2281 gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt 2341 aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg 2401 gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa 2461 gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg 2521 ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat 2581 ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc 2641 ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt 2701 gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt 2761 ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg 2821 ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg 2881 ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg 2941 aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 3001 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac 3061 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag 3121 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 3181 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 3241 gcgtttatat actagagaat tgtgagcgga taacaattga cattgtgagc ggataacaag 3301 atactgagca catactagag aaagaggaga aatactagat ggccttggtt gacggttttc 3361 ttgagctgga acgctcaagt ggaaaattgg agtggagcgc catcctccag aagatggcga 3421 gcgaccttgg attctcgaag atcctgttcg gcctgttgcc taaggacagc caggactacg 3481 agaacgcctt catcgtcggc aactacccgg ccgcctggcg cgagcattac gaccgggctg 3541 gctacgcgcg ggtcgacccg acggtcagtc actgtaccca gagcgtactg ccgattttct 3601 gggaaccgtc catctaccag acgcgaaagc agcacgagtt cttcgaggaa gcctcggccg 3661 ccggcctggt gtatgggctg accatgccgc tgcatggtgc tcgcggcgaa ctcggcgcgc 3721 tgagcctcag cgtggaagcg gaaaaccggg ccgaggccaa ccgtttcata gagtcggtcc 3781 tgccgaccct gtggatgctc aaggactacg cactgcaaag cggtgccgga ctggccttcg 3841 aacatccggt cagcaaaccg gtggttctga ccagccggga gaaggaagtg ttgcagtggt 3901 gcgccatcgg caagaccagt tgggagatat cggttatctg caactgctcg gaagccaatg 3961 tgaacttcca tatgggaaat attcggcgga agttcggtgt gacctcccgc cgcgtagcgg 4021 ccattatggc cgttaatttg ggtcttatta ctctcgctgc aaacgacgaa aactacgctt 4081 tagtagctta ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat 4141 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 4201 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //