##gff-version 3
##sequence-region BBa_S04210 1 4256
BBa_S04210	.	engineered_region	1899	4256	.	+	0	ID=BBa_S04206;Name=BBa_S04206
BBa_S04210	.	promoter	1899	1933	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_S04206
BBa_S04210	.	engineered_region	1942	4256	.	+	0	ID=BBa_S04303;Name=BBa_S04303;Parent=BBa_S04206
BBa_S04210	.	engineered_region	1942	3332	.	+	0	ID=BBa_S04302;Name=BBa_S04302;Parent=BBa_S04303
BBa_S04210	.	ribosome_entry_site	3321	3332	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04302
BBa_S04210	.	conserved_region	3325	3328	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04210	.	engineered_region	1942	3312	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121;Parent=BBa_S04302
BBa_S04210	.	promoter	3258	3311	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_S04210	.	engineered_region	3258	3311	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_S04210	.	operator	3283	3299	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_S04210	.	promoter	3300	3305	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_S04210	.	operator	3260	3276	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_S04210	.	promoter	3277	3282	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_S04210	.	terminator	3121	3249	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_S04210	.	terminator	3209	3249	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04210	.	engineered_region	3209	3249	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04210	.	stem_loop	3216	3235	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04210	.	polyA_site	3236	3249	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04210	.	stop_codon	3242	3242	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04210	.	terminator	3121	3200	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04210	.	engineered_region	3121	3200	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04210	.	stem_loop	3132	3175	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04210	.	CDS	1960	3087	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_S04210	.	engineered_region	1960	3087	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_S04210	.	engineered_tag	3088	3112	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_S04210	.	tag	3049	3087	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_S04210	.	CDS	1960	3087	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_S04210	.	ribosome_entry_site	1942	1953	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_S04210	.	conserved_region	1946	1949	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04210	.	CDS	3339	4256	.	+	0	ID=BBa_S04301;Name=BBa_S04301;Parent=BBa_S04303
BBa_S04210	.	terminator	4128	4256	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_S04301
BBa_S04210	.	terminator	4216	4256	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04210	.	engineered_region	4216	4256	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04210	.	stem_loop	4223	4242	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04210	.	polyA_site	4243	4256	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04210	.	stop_codon	4249	4249	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04210	.	terminator	4128	4207	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04210	.	engineered_region	4128	4207	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04210	.	stem_loop	4139	4182	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04210	.	CDS	3339	4119	.	+	0	ID=BBa_C0079;Name=BBa_C0079;Parent=BBa_S04301
BBa_S04210	.	engineered_tag	4095	4119	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_S04210	.	CDS	3339	4055	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_S04210	.	sequence_alteration	3818	3818	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_S04210	.	tag	4056	4088	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_S04210	.	engineered_region	1	1890	.	+	0	ID=BBa_S04215;Name=BBa_S04215
BBa_S04210	.	engineered_region	1	883	.	+	0	ID=BBa_K145201;Name=BBa_K145201;Parent=BBa_S04215
BBa_S04210	.	terminator	755	883	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K145201
BBa_S04210	.	terminator	843	883	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04210	.	engineered_region	843	883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04210	.	stem_loop	850	869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04210	.	polyA_site	870	883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04210	.	stop_codon	876	876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04210	.	terminator	755	834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04210	.	engineered_region	755	834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04210	.	stem_loop	766	809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04210	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K145201
BBa_S04210	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04210	.	CDS	62	746	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K145201
BBa_S04210	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S04210	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S04210	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S04210	.	promoter	1	35	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_K145201
BBa_S04210	.	engineered_region	892	1890	.	+	0	ID=BBa_K116640;Name=BBa_K116640;Parent=BBa_S04215
BBa_S04210	.	promoter	892	945	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K116640
BBa_S04210	.	promoter	934	939	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S04210	.	promoter	911	916	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S04210	.	engineered_region	892	945	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_S04210	.	non_covalent_binding_site	917	935	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S04210	.	non_covalent_binding_site	892	910	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S04210	.	engineered_region	954	1890	.	+	0	ID=BBa_K116626;Name=BBa_K116626;Parent=BBa_K116640
BBa_S04210	.	engineered_region	954	1753	.	+	0	ID=BBa_K116616;Name=BBa_K116616;Parent=BBa_K116626
BBa_S04210	.	CDS	973	1728	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K116616
BBa_S04210	.	engineered_region	973	974	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_S04210	.	engineered_region	973	1728	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_S04210	.	engineered_tag	1729	1753	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_S04210	.	point_mutation	1464	1464	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_S04210	.	point_mutation	1146	1146	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_S04210	.	CDS	973	1722	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_S04210	.	ribosome_entry_site	954	966	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116616
BBa_S04210	.	ribosome_entry_site	954	966	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_S04210	.	engineered_region	954	966	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_S04210	.	ribosome_entry_site	960	963	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_S04210	.	terminator	1762	1890	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K116626
BBa_S04210	.	terminator	1850	1890	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_S04210	.	engineered_region	1850	1890	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04210	.	stem_loop	1857	1876	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_S04210	.	polyA_site	1877	1890	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04210	.	stop_codon	1883	1883	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04210	.	terminator	1762	1841	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_S04210	.	engineered_region	1762	1841	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04210	.	stem_loop	1773	1816	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_S04210
ttgacagctagctcagtcctagggactatgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactac
tagagtcacacaggaaagtactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagctt
gtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgat
catttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgat
gacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttg
aaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagttt
ccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagttta
tttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaata
ataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatat
ttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaac
cgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagt
gctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactag
agttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggtgaatgtgaaac
cagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagcca
cgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaa
ctggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcgg
cgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaa
agcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccatt
gctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattt
tctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcggg
cccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgata
gcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttccca
ctgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgc
ggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggat
tttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgt
tgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattc
attaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagct
taataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactag
agaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggag
cgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggac
tacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcg
acccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagca
cgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactc
ggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgt
ggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgac
cagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcg
gaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatgg
ccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgcta
gtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata