LOCUS       BBa_S04214              1217 bp    DNA     linear       05-Nov-2024
DEFINITION  E5500:K091137
ACCESSION   BBa_S04214
VERSION     BBa_S04214.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..741
                     /label=GFP protein
     CDS             22..741
                     /label=BBa_E0040
     misc_feature    1..741
                     /label=BBa_E5500
     misc_feature    754..757
                     /label=conserved
     RBS             750..761
                     /label=BBa_B0034
     CDS             768..1016
                     /label=mnt
     misc_feature    862..864
                     /label=T
     misc_feature    1017..1049
                     /label=LVA
     misc_feature    1056..1080
                     /label=Help:Barcodes
     CDS             768..1055
                     /label=BBa_C0072
     misc_feature    750..1080
                     /label=BBa_I13025
     stem_loop       1100..1143
                     /label=stem_loop
     misc_feature    1089..1168
                     /label=BBa_B0010
     terminator      1089..1168
                     /label=BBa_B0010
     stem_loop       1184..1203
                     /label=T7 TE
     misc_feature    1210..1210
                     /label=stop
     polyA_site      1204..1217
                     /label=polya
     misc_feature    1177..1217
                     /label=BBa_B0012
     terminator      1177..1217
                     /label=BBa_B0012
     terminator      1089..1217
                     /label=BBa_B0015
     misc_feature    750..1217
                     /label=BBa_K091137
ORIGIN
        1 attaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
       61 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      121 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      181 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      241 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      301 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      361 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      421 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      481 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      541 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      601 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      661 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
      721 gatgaactat acaaataata atactagaga aagaggagaa atactagatg gcccgggatg
      781 atcctcactt caattttcgt atgccaatgg aagtaagaga gaaattgaaa tttagagcag
      841 aggcaaacgg acggagcatg aactctgagc ttttgcaaat cgtacaagat gccctaagca
      901 aaccgtcacc agtcactggg taccgcaatg atgcggaacg actcgccgat gagcagagcg
      961 agttagtgaa gaagatggtc ttcgatacac tgaaggatct ttataaaaaa accaccgctg
     1021 caaacgacga aaactacgct ttagtagctt aataaccctg atagtgctag tgtagatccc
     1081 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1141 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     1201 gcctttctgc gtttata
//