LOCUS       BBa_S04215              1890 bp    DNA     linear       05-Nov-2024
DEFINITION  K145201:K116640
ACCESSION   BBa_S04215
VERSION     BBa_S04215.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23116
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..681
                     /label=tetR
     misc_feature    682..715
                     /label=SsrA
     misc_feature    722..746
                     /label=Help:Barcodes
     CDS             62..746
                     /label=BBa_C0040
     stem_loop       766..809
                     /label=stem_loop
     misc_feature    755..834
                     /label=BBa_B0010
     terminator      755..834
                     /label=BBa_B0010
     stem_loop       850..869
                     /label=T7 TE
     misc_feature    876..876
                     /label=stop
     polyA_site      870..883
                     /label=polya
     misc_feature    843..883
                     /label=BBa_B0012
     terminator      843..883
                     /label=BBa_B0012
     terminator      755..883
                     /label=BBa_B0015
     misc_feature    1..883
                     /label=BBa_K145201
     misc_feature    892..910
                     /label=TetR 1
     misc_feature    892..945
                     /label=BBa_R0040
     promoter        911..916
                     /label=-35
     misc_feature    917..935
                     /label=TetR 2
     promoter        934..939
                     /label=-10
     promoter        892..945
                     /label=BBa_R0040
     RBS             954..966
                     /label=RBS-3\Weak
     RBS             960..963
                     /label=RBS
     misc_feature    954..966
                     /label=BBa_B0032
     RBS             954..966
                     /label=BBa_B0032
     misc_feature    973..974
                     /label=prefix
     misc_feature    1146..1146
                     /label=T
     misc_feature    1464..1464
                     /label=A
     CDS             973..1722
                     /label=luxR
     misc_feature    973..1728
                     /label=BBa_C0062
     misc_feature    1729..1753
                     /label=Help:Barcodes
     CDS             973..1728
                     /label=BBa_C0062
     misc_feature    954..1753
                     /label=BBa_K116616
     stem_loop       1773..1816
                     /label=stem_loop
     misc_feature    1762..1841
                     /label=BBa_B0010
     terminator      1762..1841
                     /label=BBa_B0010
     stem_loop       1857..1876
                     /label=T7 TE
     misc_feature    1883..1883
                     /label=stop
     polyA_site      1877..1890
                     /label=polya
     misc_feature    1850..1890
                     /label=BBa_B0012
     terminator      1850..1890
                     /label=BBa_B0012
     terminator      1762..1890
                     /label=BBa_B0015
     misc_feature    954..1890
                     /label=BBa_K116626
     misc_feature    892..1890
                     /label=BBa_K116640
ORIGIN
        1 ttgacagcta gctcagtcct agggactatg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
      781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca
      901 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagtcacaca
      961 ggaaagtact agatgaaaaa cataaatgcc gacgacacat acagaataat taataaaatt
     1021 aaagcttgta gaagcaataa tgatattaat caatgcttat ctgatatgac taaaatggta
     1081 cattgtgaat attatttact cgcgatcatt tatcctcatt ctatggttaa atctgatatt
     1141 tcaatcctag ataattaccc taaaaaatgg aggcaatatt atgatgacgc taatttaata
     1201 aaatatgatc ctatagtaga ttattctaac tccaatcatt caccaattaa ttggaatata
     1261 tttgaaaaca atgctgtaaa taaaaaatct ccaaatgtaa ttaaagaagc gaaaacatca
     1321 ggtcttatca ctgggtttag tttccctatt catacggcta acaatggctt cggaatgctt
     1381 agttttgcac attcagaaaa agacaactat atagatagtt tatttttaca tgcgtgtatg
     1441 aacataccat taattgttcc ttctctagtt gataattatc gaaaaataaa tatagcaaat
     1501 aataaatcaa acaacgattt aaccaaaaga gaaaaagaat gtttagcgtg ggcatgcgaa
     1561 ggaaaaagct cttgggatat ttcaaaaata ttaggttgca gtgagcgtac tgtcactttc
     1621 catttaacca atgcgcaaat gaaactcaat acaacaaacc gctgccaaag tatttctaaa
     1681 gcaattttaa caggagcaat tgattgccca tactttaaaa attaataaca ctgatagtgc
     1741 tagtgtagat cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1801 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1861 caccttcggg tgggcctttc tgcgtttata
//