LOCUS BBa_S04216 5725 bp DNA linear 23-Dec-2024 DEFINITION S04209:S04215:S04208:S04205 ACCESSION BBa_S04216 VERSION BBa_S04216.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 1151..1189 /label=LVA CDS 62..1189 /label=lacI-LVA misc_feature 62..1189 /label=BBa_C0012 misc_feature 1190..1214 /label=Help:Barcodes CDS 62..1189 /label=BBa_C0012 stem_loop 1234..1277 /label=stem_loop misc_feature 1223..1302 /label=BBa_B0010 terminator 1223..1302 /label=BBa_B0010 stem_loop 1318..1337 /label=T7 TE misc_feature 1344..1344 /label=stop polyA_site 1338..1351 /label=polya misc_feature 1311..1351 /label=BBa_B0012 terminator 1311..1351 /label=BBa_B0012 terminator 1223..1351 /label=BBa_B0015 misc_feature 1362..1378 /label=lac O1 promoter 1379..1384 /label=-35 misc_feature 1385..1401 /label=lac O1 promoter 1402..1407 /label=-10 misc_feature 1360..1413 /label=BBa_R0011 promoter 1360..1413 /label=BBa_R0011 misc_feature 44..1414 /label=BBa_Q04121 misc_feature 1..1414 /label=BBa_S04208 misc_feature 1427..1430 /label=conserved RBS 1423..1434 /label=BBa_B0034 misc_feature 1..1434 /label=BBa_S04209 promoter 1443..1477 /label=BBa_J23116 misc_feature 1490..1493 /label=conserved RBS 1486..1497 /label=BBa_B0034 CDS 1507..2123 /label=tetR misc_feature 2124..2157 /label=SsrA misc_feature 2164..2188 /label=Help:Barcodes CDS 1504..2188 /label=BBa_C0040 stem_loop 2208..2251 /label=stem_loop misc_feature 2197..2276 /label=BBa_B0010 terminator 2197..2276 /label=BBa_B0010 stem_loop 2292..2311 /label=T7 TE misc_feature 2318..2318 /label=stop polyA_site 2312..2325 /label=polya misc_feature 2285..2325 /label=BBa_B0012 terminator 2285..2325 /label=BBa_B0012 terminator 2197..2325 /label=BBa_B0015 misc_feature 1443..2325 /label=BBa_K145201 misc_feature 2334..2352 /label=TetR 1 misc_feature 2334..2387 /label=BBa_R0040 promoter 2353..2358 /label=-35 misc_feature 2359..2377 /label=TetR 2 promoter 2376..2381 /label=-10 promoter 2334..2387 /label=BBa_R0040 RBS 2396..2408 /label=RBS-3\Weak RBS 2402..2405 /label=RBS misc_feature 2396..2408 /label=BBa_B0032 RBS 2396..2408 /label=BBa_B0032 misc_feature 2415..2416 /label=prefix misc_feature 2588..2588 /label=T misc_feature 2906..2906 /label=A CDS 2415..3164 /label=luxR misc_feature 2415..3170 /label=BBa_C0062 misc_feature 3171..3195 /label=Help:Barcodes CDS 2415..3170 /label=BBa_C0062 misc_feature 2396..3195 /label=BBa_K116616 stem_loop 3215..3258 /label=stem_loop misc_feature 3204..3283 /label=BBa_B0010 terminator 3204..3283 /label=BBa_B0010 stem_loop 3299..3318 /label=T7 TE misc_feature 3325..3325 /label=stop polyA_site 3319..3332 /label=polya misc_feature 3292..3332 /label=BBa_B0012 terminator 3292..3332 /label=BBa_B0012 terminator 3204..3332 /label=BBa_B0015 misc_feature 2396..3332 /label=BBa_K116626 misc_feature 2334..3332 /label=BBa_K116640 misc_feature 1443..3332 /label=BBa_S04215 promoter 3341..3375 /label=BBa_J23100 misc_feature 3388..3391 /label=conserved RBS 3384..3395 /label=BBa_B0034 misc_feature 4491..4529 /label=LVA CDS 3402..4529 /label=lacI-LVA misc_feature 3402..4529 /label=BBa_C0012 misc_feature 4530..4554 /label=Help:Barcodes CDS 3402..4529 /label=BBa_C0012 stem_loop 4574..4617 /label=stem_loop misc_feature 4563..4642 /label=BBa_B0010 terminator 4563..4642 /label=BBa_B0010 stem_loop 4658..4677 /label=T7 TE misc_feature 4684..4684 /label=stop polyA_site 4678..4691 /label=polya misc_feature 4651..4691 /label=BBa_B0012 terminator 4651..4691 /label=BBa_B0012 terminator 4563..4691 /label=BBa_B0015 misc_feature 4702..4718 /label=lac O1 promoter 4719..4724 /label=-35 misc_feature 4725..4741 /label=lac O1 promoter 4742..4747 /label=-10 misc_feature 4700..4753 /label=BBa_R0011 promoter 4700..4753 /label=BBa_R0011 misc_feature 3384..4754 /label=BBa_Q04121 misc_feature 3341..4754 /label=BBa_S04208 misc_feature 4808..4825 /label=OP2 misc_feature 4868..4887 /label=OP1 promoter 4879..4884 /label=-35 promoter 4902..4907 /label=-10 promoter 4763..4919 /label=BBa_R0079 misc_feature 4932..4935 /label=conserved RBS 4928..4939 /label=BBa_B0034 misc_feature 5525..5556 /label=LVA CDS 4946..5524 /label=luxI misc_feature 4946..5563 /label=BBa_C0061 misc_feature 5564..5588 /label=Help:Barcodes CDS 4946..5563 /label=BBa_C0061 stem_loop 5608..5651 /label=stem_loop misc_feature 5597..5676 /label=BBa_B0010 terminator 5597..5676 /label=BBa_B0010 stem_loop 5692..5711 /label=T7 TE misc_feature 5718..5718 /label=stop polyA_site 5712..5725 /label=polya misc_feature 5685..5725 /label=BBa_B0012 terminator 5685..5725 /label=BBa_B0012 misc_feature 4928..5725 /label=BBa_F1610 misc_feature 4763..5725 /label=BBa_S04205 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 61 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta 121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa 181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc 241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc 301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc 361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca 421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga 481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa 541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt 601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg 661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga 721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat 781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat 841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga 901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg 961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca 1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc 1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg 1201 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 1261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 1321 tcaccttcgg gtgggccttt ctgcgtttat atactagaga attgtgagcg gataacaatt 1381 gacattgtga gcggataaca agatactgag cacatactag agaaagagga gaaatactag 1441 agttgacagc tagctcagtc ctagggacta tgctagctac tagagaaaga ggagaaatac 1501 tagatgtcca gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag 1561 gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct 1621 acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg 1681 ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta 1741 cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta 1801 catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt 1861 ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat 1921 tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa 1981 acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac 2041 caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa 2101 caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt agtagcttaa 2161 taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg 2221 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 2281 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtccctat 2341 cagtgataga gattgacatc cctatcagtg atagagatac tgagcactac tagagtcaca 2401 caggaaagta ctagatgaaa aacataaatg ccgacgacac atacagaata attaataaaa 2461 ttaaagcttg tagaagcaat aatgatatta atcaatgctt atctgatatg actaaaatgg 2521 tacattgtga atattattta ctcgcgatca tttatcctca ttctatggtt aaatctgata 2581 tttcaatcct agataattac cctaaaaaat ggaggcaata ttatgatgac gctaatttaa 2641 taaaatatga tcctatagta gattattcta actccaatca ttcaccaatt aattggaata 2701 tatttgaaaa caatgctgta aataaaaaat ctccaaatgt aattaaagaa gcgaaaacat 2761 caggtcttat cactgggttt agtttcccta ttcatacggc taacaatggc ttcggaatgc 2821 ttagttttgc acattcagaa aaagacaact atatagatag tttattttta catgcgtgta 2881 tgaacatacc attaattgtt ccttctctag ttgataatta tcgaaaaata aatatagcaa 2941 ataataaatc aaacaacgat ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg 3001 aaggaaaaag ctcttgggat atttcaaaaa tattaggttg cagtgagcgt actgtcactt 3061 tccatttaac caatgcgcaa atgaaactca atacaacaaa ccgctgccaa agtatttcta 3121 aagcaatttt aacaggagca attgattgcc catactttaa aaattaataa cactgatagt 3181 gctagtgtag atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 3241 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 3301 ctcaccttcg ggtgggcctt tctgcgttta tatactagag ttgacggcta gctcagtcct 3361 aggtacagtg ctagctacta gagaaagagg agaaatacta gatggtgaat gtgaaaccag 3421 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg 3481 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg 3541 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga 3601 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta 3661 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg 3721 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca 3781 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc 3841 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg 3901 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc 3961 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat 4021 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt 4081 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg 4141 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg 4201 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc 4261 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct 4321 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg 4381 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 4441 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag gctgcaaacg 4501 acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag 4561 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 4621 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 4681 ctgcgtttat atactagaga attgtgagcg gataacaatt gacattgtga gcggataaca 4741 agatactgag cacatactag aggcccctcg ctgagcgcgt cccggagctg ggggcaacct 4801 agctgccacc tgcttttctg ctagctattc cagcgaaaac atacagattt ccggcgaaat 4861 caaggctacc tgccagttct ggcaggtttg gccgcgggtt ctttttggta cacgaaagct 4921 actagagaaa gaggagaaat actagatgac tataatgata aaaaaatcgg attttttggc 4981 aattccatcg gaggagtata aaggtattct aagtcttcgt tatcaagtgt ttaagcaaag 5041 acttgagtgg gacttagttg tagaaaataa ccttgaatca gatgagtatg ataactcaaa 5101 tgcagaatat atttatgctt gtgatgatac tgaaaatgta agtggatgct ggcgtttatt 5161 acctacaaca ggtgattata tgctgaaaag tgtttttcct gaattgcttg gtcaacagag 5221 tgctcccaaa gatcctaata tagtcgaatt aagtcgtttt gctgtaggta aaaatagctc 5281 aaagataaat aactctgcta gtgaaattac aatgaaacta tttgaagcta tatataaaca 5341 cgctgttagt caaggtatta cagaatatgt aacagtaaca tcaacagcaa tagagcgatt 5401 tttaaagcgt attaaagttc cttgtcatcg tattggagac aaagaaattc atgtattagg 5461 tgatactaaa tcggttgtat tgtctatgcc tattaatgaa cagtttaaaa aagcagtctt 5521 aaatgctgca aacgacgaaa actacgcttt agtagcttaa taactctgat agtgctagtg 5581 tagatctcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 5641 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 5701 tcgggtgggc ctttctgcgt ttata //