LOCUS       BBa_S04219              2500 bp    DNA     linear       15-Dec-2024
DEFINITION  I763026:I763004
ACCESSION   BBa_S04219
VERSION     BBa_S04219.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    1170..1208
                     /label=LVA
     CDS             81..1208
                     /label=lacI-LVA
     misc_feature    81..1208
                     /label=BBa_C0012
     misc_feature    1209..1233
                     /label=Help:Barcodes
     CDS             81..1208
                     /label=BBa_C0012
     misc_feature    63..1233
                     /label=BBa_S0100
     misc_feature    1..1233
                     /label=BBa_I763025
     stem_loop       1253..1296
                     /label=stem_loop
     misc_feature    1242..1321
                     /label=BBa_B0010
     terminator      1242..1321
                     /label=BBa_B0010
     stem_loop       1337..1356
                     /label=T7 TE
     misc_feature    1363..1363
                     /label=stop
     polyA_site      1357..1370
                     /label=polya
     misc_feature    1330..1370
                     /label=BBa_B0012
     terminator      1330..1370
                     /label=BBa_B0012
     terminator      1242..1370
                     /label=BBa_B0015
     misc_feature    1..1370
                     /label=BBa_I763026
     CDS             1379..1466
                     /label=end of LacI coding region (inactive)
     misc_feature    1379..1578
                     /label=BBa_R0010
     misc_feature    1467..1504
                     /label=CAP binding site
     promoter        1515..1520
                     /label=-35
     promoter        1539..1544
                     /label=-10
     misc_feature    1544..1578
                     /label=LacI binding site
     misc_feature    1551..1551
                     /label=start
     promoter        1379..1578
                     /label=BBa_R0010
     misc_feature    1591..1594
                     /label=conserved
     RBS             1587..1598
                     /label=BBa_B0034
     misc_feature    1605..2324
                     /label=BBa_E0040
     misc_feature    2319..2357
                     /label=LVA
     misc_feature    2358..2363
                     /label=Double Stop
     misc_feature    1605..1607
                     /label=Start
     misc_feature    1605..2363
                     /label=BBa_J04031
     stem_loop       2383..2426
                     /label=stem_loop
     misc_feature    2372..2451
                     /label=BBa_B0010
     terminator      2372..2451
                     /label=BBa_B0010
     stem_loop       2467..2486
                     /label=T7 TE
     misc_feature    2493..2493
                     /label=stop
     polyA_site      2487..2500
                     /label=polya
     misc_feature    2460..2500
                     /label=BBa_B0012
     terminator      2460..2500
                     /label=BBa_B0012
     misc_feature    1379..2500
                     /label=BBa_I763004
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag
      121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
      181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
      241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
      301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
      361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
      421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
      481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
      541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
      601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
      661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
      721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
      781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
      841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
      901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
      961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag
     1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg
     1261 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1321 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagca
     1381 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     1441 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     1501 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     1561 ggataacaat ttcacacata ctagagaaag aggagaaata ctagatgcgt aaaggagaag
     1621 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
     1681 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat
     1741 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt
     1801 atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga
     1861 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact
     1921 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
     1981 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
     2041 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca
     2101 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata
     2161 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
     2221 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
     2281 ctgctgggat tacacatggc atggatgaac tatacaaaag gcctgctgca aacgacgaaa
     2341 actacgcttt agtagcttaa taatactaga gccaggcatc aaataaaacg aaaggctcag
     2401 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     2461 cacactggct caccttcggg tgggcctttc tgcgtttata
//