LOCUS BBa_S04220 3391 bp DNA linear 22-Nov-2024 DEFINITION I739001:S04219 ACCESSION BBa_S04220 VERSION BBa_S04220.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23105 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 65..681 /label=tetR misc_feature 682..715 /label=SsrA misc_feature 722..746 /label=Help:Barcodes CDS 62..746 /label=BBa_C0040 stem_loop 766..809 /label=stem_loop misc_feature 755..834 /label=BBa_B0010 terminator 755..834 /label=BBa_B0010 stem_loop 850..869 /label=T7 TE misc_feature 876..876 /label=stop polyA_site 870..883 /label=polya misc_feature 843..883 /label=BBa_B0012 terminator 843..883 /label=BBa_B0012 terminator 755..883 /label=BBa_B0015 misc_feature 1..883 /label=BBa_I739001 misc_feature 892..910 /label=TetR 1 misc_feature 892..945 /label=BBa_R0040 promoter 911..916 /label=-35 misc_feature 917..935 /label=TetR 2 promoter 934..939 /label=-10 promoter 892..945 /label=BBa_R0040 misc_feature 958..961 /label=conserved RBS 954..965 /label=BBa_B0034 misc_feature 2061..2099 /label=LVA CDS 972..2099 /label=lacI-LVA misc_feature 972..2099 /label=BBa_C0012 misc_feature 2100..2124 /label=Help:Barcodes CDS 972..2099 /label=BBa_C0012 misc_feature 954..2124 /label=BBa_S0100 misc_feature 892..2124 /label=BBa_I763025 stem_loop 2144..2187 /label=stem_loop misc_feature 2133..2212 /label=BBa_B0010 terminator 2133..2212 /label=BBa_B0010 stem_loop 2228..2247 /label=T7 TE misc_feature 2254..2254 /label=stop polyA_site 2248..2261 /label=polya misc_feature 2221..2261 /label=BBa_B0012 terminator 2221..2261 /label=BBa_B0012 terminator 2133..2261 /label=BBa_B0015 misc_feature 892..2261 /label=BBa_I763026 CDS 2270..2357 /label=end of LacI coding region (inactive) misc_feature 2270..2469 /label=BBa_R0010 misc_feature 2358..2395 /label=CAP binding site promoter 2406..2411 /label=-35 promoter 2430..2435 /label=-10 misc_feature 2435..2469 /label=LacI binding site misc_feature 2442..2442 /label=start promoter 2270..2469 /label=BBa_R0010 misc_feature 2482..2485 /label=conserved RBS 2478..2489 /label=BBa_B0034 misc_feature 2496..3215 /label=BBa_E0040 misc_feature 3210..3248 /label=LVA misc_feature 3249..3254 /label=Double Stop misc_feature 2496..2498 /label=Start misc_feature 2496..3254 /label=BBa_J04031 stem_loop 3274..3317 /label=stem_loop misc_feature 3263..3342 /label=BBa_B0010 terminator 3263..3342 /label=BBa_B0010 stem_loop 3358..3377 /label=T7 TE misc_feature 3384..3384 /label=stop polyA_site 3378..3391 /label=polya misc_feature 3351..3391 /label=BBa_B0012 terminator 3351..3391 /label=BBa_B0012 misc_feature 2270..3391 /label=BBa_I763004 misc_feature 892..3391 /label=BBa_I763027 ORIGIN 1 tttacggcta gctcagtcct aggtactatg ctagctacta gagaaagagg agaaatacta 61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca 901 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagaaagagg 961 agaaatacta gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg 1021 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa 1081 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac 1141 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc 1201 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg 1261 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc 1321 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca 1381 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga 1441 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc 1501 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg 1561 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag 1621 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga 1681 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa 1741 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg 1801 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc 1861 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga 1921 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata 1981 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 2041 cccgactgga aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac 2101 tctgatagtg ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca 2161 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 2221 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagc aatacgcaaa 2281 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 2341 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc 2401 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa 2461 tttcacacat actagagaaa gaggagaaat actagatgcg taaaggagaa gaacttttca 2521 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg 2581 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca 2641 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc 2701 aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc 2761 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac 2821 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg 2881 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca 2941 atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac 3001 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg 3061 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga 3121 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga 3181 ttacacatgg catggatgaa ctatacaaaa ggcctgctgc aaacgacgaa aactacgctt 3241 tagtagctta ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 3301 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 3361 tcaccttcgg gtgggccttt ctgcgtttat a //