LOCUS BBa_S04221 1274 bp DNA linear 05-Nov-2024 DEFINITION R0051:S04214 ACCESSION BBa_S04221 VERSION BBa_S04221.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 RBS 58..72 /label=RBS-1\Strong RBS 65..69 /label=RBS misc_feature 58..72 /label=BBa_B0030 RBS 58..72 /label=BBa_B0030 CDS 79..798 /label=GFP protein CDS 79..798 /label=BBa_E0040 misc_feature 58..798 /label=BBa_E5500 misc_feature 811..814 /label=conserved RBS 807..818 /label=BBa_B0034 CDS 825..1073 /label=mnt misc_feature 919..921 /label=T misc_feature 1074..1106 /label=LVA misc_feature 1113..1137 /label=Help:Barcodes CDS 825..1112 /label=BBa_C0072 misc_feature 807..1137 /label=BBa_I13025 stem_loop 1157..1200 /label=stem_loop misc_feature 1146..1225 /label=BBa_B0010 terminator 1146..1225 /label=BBa_B0010 stem_loop 1241..1260 /label=T7 TE misc_feature 1267..1267 /label=stop polyA_site 1261..1274 /label=polya misc_feature 1234..1274 /label=BBa_B0012 terminator 1234..1274 /label=BBa_B0012 terminator 1146..1274 /label=BBa_B0015 misc_feature 807..1274 /label=BBa_K091137 misc_feature 58..1274 /label=BBa_S04214 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagatt 61 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt 121 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa 181 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct 241 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac 301 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag 361 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt 421 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga 481 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca 541 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga 601 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt 661 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag 721 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat 781 gaactataca aataataata ctagagaaag aggagaaata ctagatggcc cgggatgatc 841 ctcacttcaa ttttcgtatg ccaatggaag taagagagaa attgaaattt agagcagagg 901 caaacggacg gagcatgaac tctgagcttt tgcaaatcgt acaagatgcc ctaagcaaac 961 cgtcaccagt cactgggtac cgcaatgatg cggaacgact cgccgatgag cagagcgagt 1021 tagtgaagaa gatggtcttc gatacactga aggatcttta taaaaaaacc accgctgcaa 1081 acgacgaaaa ctacgcttta gtagcttaat aaccctgata gtgctagtgt agatccctac 1141 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 1201 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 1261 tttctgcgtt tata //