LOCUS       BBa_S04221              1274 bp    DNA     linear       22-Dec-2024
DEFINITION  R0051:S04214
ACCESSION   BBa_S04221
VERSION     BBa_S04221.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     RBS             58..72
                     /label=RBS-1\Strong
     RBS             65..69
                     /label=RBS
     misc_feature    58..72
                     /label=BBa_B0030
     RBS             58..72
                     /label=BBa_B0030
     CDS             79..798
                     /label=GFP protein
     CDS             79..798
                     /label=BBa_E0040
     misc_feature    58..798
                     /label=BBa_E5500
     misc_feature    811..814
                     /label=conserved
     RBS             807..818
                     /label=BBa_B0034
     CDS             825..1073
                     /label=mnt
     misc_feature    919..921
                     /label=T
     misc_feature    1074..1106
                     /label=LVA
     misc_feature    1113..1137
                     /label=Help:Barcodes
     CDS             825..1112
                     /label=BBa_C0072
     misc_feature    807..1137
                     /label=BBa_I13025
     stem_loop       1157..1200
                     /label=stem_loop
     misc_feature    1146..1225
                     /label=BBa_B0010
     terminator      1146..1225
                     /label=BBa_B0010
     stem_loop       1241..1260
                     /label=T7 TE
     misc_feature    1267..1267
                     /label=stop
     polyA_site      1261..1274
                     /label=polya
     misc_feature    1234..1274
                     /label=BBa_B0012
     terminator      1234..1274
                     /label=BBa_B0012
     terminator      1146..1274
                     /label=BBa_B0015
     misc_feature    807..1274
                     /label=BBa_K091137
     misc_feature    58..1274
                     /label=BBa_S04214
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagatt
       61 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
      121 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      181 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      241 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      301 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      361 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      421 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      481 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      541 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      601 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      661 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      721 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      781 gaactataca aataataata ctagagaaag aggagaaata ctagatggcc cgggatgatc
      841 ctcacttcaa ttttcgtatg ccaatggaag taagagagaa attgaaattt agagcagagg
      901 caaacggacg gagcatgaac tctgagcttt tgcaaatcgt acaagatgcc ctaagcaaac
      961 cgtcaccagt cactgggtac cgcaatgatg cggaacgact cgccgatgag cagagcgagt
     1021 tagtgaagaa gatggtcttc gatacactga aggatcttta taaaaaaacc accgctgcaa
     1081 acgacgaaaa ctacgcttta gtagcttaat aaccctgata gtgctagtgt agatccctac
     1141 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1201 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     1261 tttctgcgtt tata
//