BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_S04235 1 BBa_S04235 K237000:B0015 2009-08-02T11:00:00Z 2015-05-08T01:14:37Z false false _9_ 0 4087 9 It's complicated true false Marc T. K. Nielsen component2014712 1 BBa_K237000 component2014713 1 BBa_B0010 component2014715 1 BBa_B0012 annotation2014715 1 BBa_B0012 range2014715 1 127 167 annotation2014713 1 BBa_B0010 range2014713 1 39 118 annotation2014712 1 BBa_K237000 range2014712 1 1 30 BBa_K237000 1 RIP RIP - Quorum sensing inhibitor of Staphylococcus spp. (Including MRSA) 2009-08-02T11:00:00Z 2015-05-08T01:11:36Z The RIP peptide is produced by coagulase negative staphylococcus (suggested to be S. warnerii or S. xylosus) (16,19) RIP or RNAIII inhibiting peptide is a heptapeptide capable of severely reducing the Qourum sensing(QS) abilities of Staphylococcus species. RIP effectively works as an antibiotic in its inhibition of bacterial growth. RIP has the sequence YSPXTNF and has been tested extensively in a synthetic (as in organic) form for ability to inhibit bacterial infections in mice. References will come later. This is a pure RIP Brick, it contains no signal sequence and we can at this point in our project not verify that it will be exported by our Coli's false false _333_ 0 4087 9 It's complicated true This sequence is reverse-engineered from the amino-acid sequence by using the commonly used codons of E. Coli. It is designed to provide a big output of peptide. We just hope that this output won't kill the cell. false Marc T. K. Nielsen annotation2014625 1 Stop range2014625 1 25 29 annotation2014624 1 Start range2014624 1 1 3 annotation2014626 1 YSPWTNF range2014626 1 4 24 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_S04235_sequence 1 atgtattctccgtggaccaacttttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K237000_sequence 1 atgtattctccgtggaccaacttttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z