LOCUS       BBa_S04250               922 bp    DNA     linear       15-Dec-2024
DEFINITION  S04248:B0017
ACCESSION   BBa_S04250
VERSION     BBa_S04250.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23109
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_S04240
     CDS             65..681
                     /label=tetR
     misc_feature    682..715
                     /label=SsrA
     misc_feature    722..746
                     /label=Help:Barcodes
     CDS             62..746
                     /label=BBa_C0040
     misc_feature    1..746
                     /label=BBa_S04248
     stem_loop       766..809
                     /label=stem_loop
     misc_feature    755..834
                     /label=BBa_B0010
     terminator      755..834
                     /label=BBa_B0010
     stem_loop       854..897
                     /label=stem_loop
     misc_feature    843..922
                     /label=BBa_B0010
     terminator      843..922
                     /label=BBa_B0010
     terminator      755..922
                     /label=BBa_B0017
ORIGIN
        1 tttacagcta gctcagtcct agggactgtg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
      781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      841 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      901 ttgtttgtcg gtgaacgctc tc
//