##gff-version 3
##sequence-region BBa_S04250 1 922
BBa_S04250	.	engineered_region	1	746	.	+	0	ID=BBa_S04248;Name=BBa_S04248
BBa_S04250	.	engineered_region	1	55	.	+	0	ID=BBa_S04240;Name=BBa_S04240;Parent=BBa_S04248
BBa_S04250	.	promoter	1	35	.	+	0	ID=BBa_J23109;Name=BBa_J23109;Parent=BBa_S04240
BBa_S04250	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04240
BBa_S04250	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04250	.	CDS	62	746	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S04248
BBa_S04250	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_S04250	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_S04250	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_S04250	.	terminator	755	922	.	+	0	ID=BBa_B0017;Name=BBa_B0017
BBa_S04250	.	terminator	843	922	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0017
BBa_S04250	.	stem_loop	854	897	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04250	.	engineered_region	843	922	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04250	.	terminator	755	834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0017
BBa_S04250	.	stem_loop	766	809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04250	.	engineered_region	755	834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_S04250
tttacagctagctcagtcctagggactgtgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc