LOCUS       BBa_S04253              1971 bp    DNA     linear       15-Nov-2024
DEFINITION  J01101:I763011
ACCESSION   BBa_S04253
VERSION     BBa_S04253.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             84..791
                     /label=cI lambda
     misc_feature    792..824
                     /label=LVA
     misc_feature    831..855
                     /label=Help:Barcodes
     CDS             81..830
                     /label=BBa_C0051
     stem_loop       875..918
                     /label=stem_loop
     misc_feature    864..943
                     /label=BBa_B0010
     terminator      864..943
                     /label=BBa_B0010
     stem_loop       959..978
                     /label=T7 TE
     misc_feature    985..985
                     /label=stop
     polyA_site      979..992
                     /label=polya
     misc_feature    952..992
                     /label=BBa_B0012
     terminator      952..992
                     /label=BBa_B0012
     misc_feature    1..992
                     /label=BBa_J01101
     promoter        1038..1043
                     /label=-10
     promoter        1015..1020
                     /label=-35
     misc_feature    1025..1041
                     /label=OR1
     misc_feature    1001..1017
                     /label=OR2
     misc_feature    1001..1049
                     /label=BBa_R0051
     promoter        1001..1049
                     /label=BBa_R0051
     misc_feature    1062..1065
                     /label=conserved
     RBS             1058..1069
                     /label=BBa_B0034
     misc_feature    1076..1795
                     /label=BBa_E0040
     misc_feature    1790..1828
                     /label=LVA
     misc_feature    1829..1834
                     /label=Double Stop
     misc_feature    1076..1078
                     /label=Start
     misc_feature    1076..1834
                     /label=BBa_J04031
     stem_loop       1854..1897
                     /label=stem_loop
     misc_feature    1843..1922
                     /label=BBa_B0010
     terminator      1843..1922
                     /label=BBa_B0010
     stem_loop       1938..1957
                     /label=T7 TE
     misc_feature    1964..1964
                     /label=stop
     polyA_site      1958..1971
                     /label=polya
     misc_feature    1931..1971
                     /label=BBa_B0012
     terminator      1931..1971
                     /label=BBa_B0012
     misc_feature    1001..1971
                     /label=BBa_I763011
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg
      121 aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc
      181 aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg
      241 gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg
      301 ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta
      361 tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga
      421 tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa
      481 ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac
      541 caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg
      601 ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga
      661 aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga
      721 tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag
      781 agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt
      841 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      901 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      961 ctcaccttcg ggtgggcctt tctgcgttta tatactagag taacaccgtg cgtgttgact
     1021 attttacctc tggcggtgat aatggttgct actagagaaa gaggagaaat actagatgcg
     1081 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
     1141 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
     1201 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
     1261 tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga
     1321 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
     1381 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
     1441 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
     1501 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
     1561 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
     1621 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
     1681 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
     1741 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaaa ggcctgctgc
     1801 aaacgacgaa aactacgctt tagtagctta ataatactag agccaggcat caaataaaac
     1861 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1921 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//