BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K196003 1 HfsH HfsH protein from <i>Caulobacter crescentus</i> 2009-08-11T11:00:00Z 2015-05-08T01:11:17Z This sequence comes from Caulobacter crescentus. Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are different proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see this page. In our project, we would like this glue to be produced by Escherichia coli. As E. coli does have homolog genes for many of these proteins, but not for HfsG and HfsH, we decided to create a plasmid including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase. Here you have HfsH. false false _309_ 0 4813 9 It's complicated false As many mutations were needed to make the part compatible with the standard 10, we decided to make it synthetized by GeneArt. false Laetitia Warny BBa_S04254 1 BBa_S04254 K196004:B0015 2009-08-12T11:00:00Z 2015-05-08T01:14:37Z false true _9_ 0 4813 9 It's complicated false false Laetitia Warny component2017301 1 BBa_B0012 component2017298 1 BBa_K196003 component2017299 1 BBa_B0010 component2017297 1 BBa_K196002 annotation2017298 1 BBa_K196003 range2017298 1 940 1716 annotation2017301 1 BBa_B0012 range2017301 1 1813 1853 annotation2017297 1 BBa_K196002 range2017297 1 1 933 annotation2017299 1 BBa_B0010 range2017299 1 1725 1804 BBa_K196002 1 HfsG HfsG protein from <i>Caulobacter crescentus</i> 2009-08-10T11:00:00Z 2015-05-08T01:11:17Z This sequence comes from Caulobacter crescentus. Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are differents proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see this page. In our project, we wanted this glue to be produced by Escherichia coli. As E. coli does have homologs for many of these proteins, but not for HfsG and HfsH, we decided to create a BB including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase. false false _309_ 0 4813 9 It's complicated false As many mutations were needed to make the part compatible with the standard 10, we decided to make it synthetized by GeneArt. false Laetitia Warny BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K196003_sequence 1 atgccgatggagttcgaaaaagtagatgcttatgaaccagaccgcagcctgaaaggcaaactgcgtcgccgtctgatccgtctggcacaccgccgtccagcaaaagtggctctggaacgtccgatggtgtctttctccttcgacgacgcgccagcaactgcttgcgaagctggcgcacgtgctctggaagctcgtggcctgcgtggcacctactatttcgctgctggtctgaccggccgtgacggccctatgggccgctatgcaactggtgaggacgcacgtcgtctgcacgaagccggtcacgaaatcgcttgccacacctactcccacctggattgtggtcagtcttctcagaccgaaaccctggctgatgtcgatcgtaatgccgaaagcctggcggcttggggtgcaggcgatccggtgtcttttgcctacccgtacggtgatgtggctgctccggctaaaacggctctgtctggtcgttttaaaactctgcgcgctctgcaccacggcctgatcaccgacggcgcagatctgaaccagactccggcagtaggcatcgaaggtgaagatggtgaaaccgttgccaaggcatggctggataaggcgaaggcacgtaaagcctggctgatcctgtatacgcacgacgtcgcaggccagcctagccagtggggttgcaccacggaagcgctggaacgcctgatcgaccgtgctctggcggacggcttcgacgttgtgacggtagccgaaggttctcgtcgtatcggcctgtaataa BBa_K196002_sequence 1 atgaacgcccctgtaaatgaactgcgcctggagaatgctgcatgggcggcggcacagccgcgtctgtccgtgctgatccctaccttccgtgatgatccgtctgctctgctgaaggcgctggaccacaccaacgcagcggttgaagtcgtggtgctggacgatggtggcggcgacgatgctctggcggaacgcactgcgcgtcgcatcgagaaaatgcgtactccagcccgcttcgtacgtctgagccgtaacgaaggtcgtgccaaaggtcgtaaccgcctggcgagccatgctcgtggtggccacttcctgttcctggactctgacatgctgcctgacaccccggactttctggaccgttggtctgctgttgccgacaccggtgccgcggttgctttcggtggtttcaccctggaccagaccccgcagcgcccggaacatgctctgcatcgtgctatggcactgaaatctgactgcacgccggctccagaacgtgcgaaggccccggaaaaacacgttttcacgtccaacctgctggttcgtcgtgatgtattcgaaactgtgggttttgatgaaggtttctccggttggggttgggaagacgtcgaatgggccatgcgtgttgcgcgtcaacacccgatcctgcatatcgataacactgccacccacctgggcctggacccagcgccagtaatggcagccaaatatgaacaatctgccgcaaactttgcacgtgttgtcgcgagccaccgcgacgttgttagcgcatatccgtcttacaaggtagcgaaactgctgaaagcagtgccgctgatctccgtttggcgtccgctgctgaaacaggtcgcactggccgaagcggcaccggtaagcctgcgtgcattcgccatgcgtctgtaccgtgctgcgctgtacagcgaagctgtttaataa BBa_S04254_sequence 1 atgaacgcccctgtaaatgaactgcgcctggagaatgctgcatgggcggcggcacagccgcgtctgtccgtgctgatccctaccttccgtgatgatccgtctgctctgctgaaggcgctggaccacaccaacgcagcggttgaagtcgtggtgctggacgatggtggcggcgacgatgctctggcggaacgcactgcgcgtcgcatcgagaaaatgcgtactccagcccgcttcgtacgtctgagccgtaacgaaggtcgtgccaaaggtcgtaaccgcctggcgagccatgctcgtggtggccacttcctgttcctggactctgacatgctgcctgacaccccggactttctggaccgttggtctgctgttgccgacaccggtgccgcggttgctttcggtggtttcaccctggaccagaccccgcagcgcccggaacatgctctgcatcgtgctatggcactgaaatctgactgcacgccggctccagaacgtgcgaaggccccggaaaaacacgttttcacgtccaacctgctggttcgtcgtgatgtattcgaaactgtgggttttgatgaaggtttctccggttggggttgggaagacgtcgaatgggccatgcgtgttgcgcgtcaacacccgatcctgcatatcgataacactgccacccacctgggcctggacccagcgccagtaatggcagccaaatatgaacaatctgccgcaaactttgcacgtgttgtcgcgagccaccgcgacgttgttagcgcatatccgtcttacaaggtagcgaaactgctgaaagcagtgccgctgatctccgtttggcgtccgctgctgaaacaggtcgcactggccgaagcggcaccggtaagcctgcgtgcattcgccatgcgtctgtaccgtgctgcgctgtacagcgaagctgtttaataatactagatgccgatggagttcgaaaaagtagatgcttatgaaccagaccgcagcctgaaaggcaaactgcgtcgccgtctgatccgtctggcacaccgccgtccagcaaaagtggctctggaacgtccgatggtgtctttctccttcgacgacgcgccagcaactgcttgcgaagctggcgcacgtgctctggaagctcgtggcctgcgtggcacctactatttcgctgctggtctgaccggccgtgacggccctatgggccgctatgcaactggtgaggacgcacgtcgtctgcacgaagccggtcacgaaatcgcttgccacacctactcccacctggattgtggtcagtcttctcagaccgaaaccctggctgatgtcgatcgtaatgccgaaagcctggcggcttggggtgcaggcgatccggtgtcttttgcctacccgtacggtgatgtggctgctccggctaaaacggctctgtctggtcgttttaaaactctgcgcgctctgcaccacggcctgatcaccgacggcgcagatctgaaccagactccggcagtaggcatcgaaggtgaagatggtgaaaccgttgccaaggcatggctggataaggcgaaggcacgtaaagcctggctgatcctgtatacgcacgacgtcgcaggccagcctagccagtggggttgcaccacggaagcgctggaacgcctgatcgaccgtgctctggcggacggcttcgacgttgtgacggtagccgaaggttctcgtcgtatcggcctgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z