BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_S04269
1
BBa_S04269
B0030:S04268
2009-08-25T11:00:00Z
2015-05-08T01:14:37Z
false
false
_9_
0
3171
9
Not in stock
false
false
Roxanne Shank
component2061161
1
BBa_B0012
component2061156
1
BBa_B0030
component2061158
1
BBa_K249016
component2061159
1
BBa_B0010
annotation2061156
1
BBa_B0030
range2061156
1
1
15
annotation2061158
1
BBa_K249016
range2061158
1
22
207
annotation2061161
1
BBa_B0012
range2061161
1
304
344
annotation2061159
1
BBa_B0010
range2061159
1
216
295
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_K249016
1
BBa_K249016
mms6
2009-08-25T11:00:00Z
2015-05-08T01:11:40Z
A Science Paper?
mms6 is an enzyme which creates nanoparticles.
false
false
_242_342_
0
3171
9
It's complicated
false
point mutations of EcoRI and PstI sites?
false
Roxanne Shank
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_S04269_sequence
1
attaaagaggagaaatactagatggtcggtggaaccatctggaccggtaaggggctgggcctcggtctgggtctcggtctgggcgcgtgggggccgatcattctcggcgttgttggcgccggggcggtttacgcatatatgaagagccgtgatatcgaatcggcgcagagcgacgaggaagtcgaactgcgcgacgcgctggcctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K249016_sequence
1
atggtcggtggaaccatctggaccggtaaggggctgggcctcggtctgggtctcggtctgggcgcgtgggggccgatcattctcggcgttgttggcgccggggcggtttacgcatatatgaagagccgtgatatcgaatcggcgcagagcgacgaggaagtcgaactgcgcgacgcgctggcctga
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z