BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986785 1 -35 range1986785 1 20 25 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986787 1 -10 range1986787 1 43 48 annotation1986786 1 TetR 2 range1986786 1 26 44 BBa_S04288 1 BBa_S04288 S04287:B0015 2009-10-09T11:00:00Z 2015-05-08T01:14:38Z false false _9_ 0 4260 9 Not in stock false false Cihan Tastan component2222401 1 BBa_B0015 component2222394 1 BBa_S04287 annotation2222394 1 BBa_S04287 range2222394 1 1 1805 annotation2222401 1 BBa_B0015 range2222401 1 1814 1942 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_S04287 1 BBa_S04287 S04286:K258100 2009-10-09T11:00:00Z 2015-05-08T01:14:38Z false false _9_ 0 4260 9 Not in stock false false Cihan Tastan component2033307 1 BBa_K258100 component2033306 1 BBa_B0034 component2033300 1 BBa_R0040 annotation2033300 1 BBa_R0040 range2033300 1 1 54 annotation2033306 1 BBa_B0034 range2033306 1 63 74 annotation2033307 1 BBa_K258100 range2033307 1 81 1805 BBa_K258100 1 BBa_K258100 PrtD domain of ABC transporter of Erwinia chrysanthemi 2009-10-09T11:00:00Z 2015-05-08T01:11:42Z E. chrysanthemi ABC transporter PrtDEF might function well in the phylogenetically-neighboring genus E. coli.On the basis of the apparent molecular weights we tentatively assigned the 60 kd protein to the prtD gene product (protein PrtD). The predicted amino acid sequence of the PrtD protein indicates that this protein has a putative ATP binding site, located in the C-terminal half of the protein, which corresponds to the consensus sequence proposed for ATP binding proteins. false false _358_ 0 4260 9 Not in stock false The gene actually will be fully function with other two genes which are PrtD and PrtE false Cihan Tastan BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_S04288_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaacgcttcctctgaacgcgatcgtagcctgttcggcgtgctgcgccagttccgccgctccttttggtctgttggtatcttctctgcggtgatcaacgttctgatgctggcaccgtctgtttatatgctgcaggtatatgaccgtgttctggcttctggtaatggcatcaccctgctgatgctgaccctgctgatggcaggtctgtgcgccttcatgggtgcactggaatgggttcgctccctgctggtcgttcgcctgggtactcgtatcgatctggcactgaaccaggacgtattcaatgcggcctttgcgcgtaacctggaagcgggtgacggtcgtgcgggtctggccctgaccgatctgaccctgctgcgtcagttcatcacgggtaacgcgctgttcgctttcttcgatgttccgtggttcccgctgttcctgctggtgctgttcctgctgcacccatggctgggtatgctggccctgggcggtactgtggtgccgggtggtgttggcctggcggaaccggcgtccgaccagtctactgctggtggcagcaaccagcagtctcagcaggccactcatctggccgacgctcagctgcgtaacgcggatgttattgaagctatgggcatgctgggcaacctgcgtcgtcgttggctggcacgccactatcgtttcatttccctgcaaaacctggcaagcgaacgtgctgcagcggtcggcggtgcgagcaaatattctcgcattgctctgcaaagcctgatgctgggcctgggtgcactgctggcgatcgacggtaaaatcacccctggcatgatgattgctggcagcatcctggttggtcgtgtgctgagcccgatcgatcagctgatcggtgtatggaaacagtggtcttctgcccgtatcgcatggcagcgcctgacgcgcctgattgctgcgtatccgccgcgtccggcggctatggcactgcctgcaccggaaggccacctgagcgtagaacaggtttctctgcgtaccgctcagggcaacacccgcctgcaaaacatccacttctctctgcaggctggtgaaaccctggtgatcctgggcgcatctggttctggcaaatcctccctggctcgtctgctggttggcgcgcagtctccgacccagggtaaagtacgtctggacggcgcagatctgaatcaggttgacaaaaacactttcggcccgacgatcggctatctgccgcaggatgttcagctgtttaaaggctccctggcagagaacattgcgcgtttcggtgacgccgacccggaaaaagttgttgctgcggccaaactggcgggcgtgcacgaactgatcctgtctctgccgaacggttacgataccgaactgggcgacggtggtggtggcctgagcggcggtcaacgccagcgtatcggtctggctcgtgccatgtacggcgatccgtgcctgctgatcctggacgaaccgaacgcttccctggatagcgaaggcgaccaggctctgatgcaagcaattgttgcactgcaaaaacgtggcgcgaccgtagttctgatcacccaccgccctgcactgactaccctggcccagaaaattctgatcctgcacgaaggtcagcagcagcgtatgggtctggcgcgcgacgtgctgacggaactgcaacagcgttccgctgctaaccaggcacgtatgaacccgactgcggccatgcctcagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K258100_sequence 1 atgaacgcttcctctgaacgcgatcgtagcctgttcggcgtgctgcgccagttccgccgctccttttggtctgttggtatcttctctgcggtgatcaacgttctgatgctggcaccgtctgtttatatgctgcaggtatatgaccgtgttctggcttctggtaatggcatcaccctgctgatgctgaccctgctgatggcaggtctgtgcgccttcatgggtgcactggaatgggttcgctccctgctggtcgttcgcctgggtactcgtatcgatctggcactgaaccaggacgtattcaatgcggcctttgcgcgtaacctggaagcgggtgacggtcgtgcgggtctggccctgaccgatctgaccctgctgcgtcagttcatcacgggtaacgcgctgttcgctttcttcgatgttccgtggttcccgctgttcctgctggtgctgttcctgctgcacccatggctgggtatgctggccctgggcggtactgtggtgccgggtggtgttggcctggcggaaccggcgtccgaccagtctactgctggtggcagcaaccagcagtctcagcaggccactcatctggccgacgctcagctgcgtaacgcggatgttattgaagctatgggcatgctgggcaacctgcgtcgtcgttggctggcacgccactatcgtttcatttccctgcaaaacctggcaagcgaacgtgctgcagcggtcggcggtgcgagcaaatattctcgcattgctctgcaaagcctgatgctgggcctgggtgcactgctggcgatcgacggtaaaatcacccctggcatgatgattgctggcagcatcctggttggtcgtgtgctgagcccgatcgatcagctgatcggtgtatggaaacagtggtcttctgcccgtatcgcatggcagcgcctgacgcgcctgattgctgcgtatccgccgcgtccggcggctatggcactgcctgcaccggaaggccacctgagcgtagaacaggtttctctgcgtaccgctcagggcaacacccgcctgcaaaacatccacttctctctgcaggctggtgaaaccctggtgatcctgggcgcatctggttctggcaaatcctccctggctcgtctgctggttggcgcgcagtctccgacccagggtaaagtacgtctggacggcgcagatctgaatcaggttgacaaaaacactttcggcccgacgatcggctatctgccgcaggatgttcagctgtttaaaggctccctggcagagaacattgcgcgtttcggtgacgccgacccggaaaaagttgttgctgcggccaaactggcgggcgtgcacgaactgatcctgtctctgccgaacggttacgataccgaactgggcgacggtggtggtggcctgagcggcggtcaacgccagcgtatcggtctggctcgtgccatgtacggcgatccgtgcctgctgatcctggacgaaccgaacgcttccctggatagcgaaggcgaccaggctctgatgcaagcaattgttgcactgcaaaaacgtggcgcgaccgtagttctgatcacccaccgccctgcactgactaccctggcccagaaaattctgatcctgcacgaaggtcagcagcagcgtatgggtctggcgcgcgacgtgctgacggaactgcaacagcgttccgctgctaaccaggcacgtatgaacccgactgcggccatgcctcag BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_S04287_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaacgcttcctctgaacgcgatcgtagcctgttcggcgtgctgcgccagttccgccgctccttttggtctgttggtatcttctctgcggtgatcaacgttctgatgctggcaccgtctgtttatatgctgcaggtatatgaccgtgttctggcttctggtaatggcatcaccctgctgatgctgaccctgctgatggcaggtctgtgcgccttcatgggtgcactggaatgggttcgctccctgctggtcgttcgcctgggtactcgtatcgatctggcactgaaccaggacgtattcaatgcggcctttgcgcgtaacctggaagcgggtgacggtcgtgcgggtctggccctgaccgatctgaccctgctgcgtcagttcatcacgggtaacgcgctgttcgctttcttcgatgttccgtggttcccgctgttcctgctggtgctgttcctgctgcacccatggctgggtatgctggccctgggcggtactgtggtgccgggtggtgttggcctggcggaaccggcgtccgaccagtctactgctggtggcagcaaccagcagtctcagcaggccactcatctggccgacgctcagctgcgtaacgcggatgttattgaagctatgggcatgctgggcaacctgcgtcgtcgttggctggcacgccactatcgtttcatttccctgcaaaacctggcaagcgaacgtgctgcagcggtcggcggtgcgagcaaatattctcgcattgctctgcaaagcctgatgctgggcctgggtgcactgctggcgatcgacggtaaaatcacccctggcatgatgattgctggcagcatcctggttggtcgtgtgctgagcccgatcgatcagctgatcggtgtatggaaacagtggtcttctgcccgtatcgcatggcagcgcctgacgcgcctgattgctgcgtatccgccgcgtccggcggctatggcactgcctgcaccggaaggccacctgagcgtagaacaggtttctctgcgtaccgctcagggcaacacccgcctgcaaaacatccacttctctctgcaggctggtgaaaccctggtgatcctgggcgcatctggttctggcaaatcctccctggctcgtctgctggttggcgcgcagtctccgacccagggtaaagtacgtctggacggcgcagatctgaatcaggttgacaaaaacactttcggcccgacgatcggctatctgccgcaggatgttcagctgtttaaaggctccctggcagagaacattgcgcgtttcggtgacgccgacccggaaaaagttgttgctgcggccaaactggcgggcgtgcacgaactgatcctgtctctgccgaacggttacgataccgaactgggcgacggtggtggtggcctgagcggcggtcaacgccagcgtatcggtctggctcgtgccatgtacggcgatccgtgcctgctgatcctggacgaaccgaacgcttccctggatagcgaaggcgaccaggctctgatgcaagcaattgttgcactgcaaaaacgtggcgcgaccgtagttctgatcacccaccgccctgcactgactaccctggcccagaaaattctgatcctgcacgaaggtcagcagcagcgtatgggtctggcgcgcgacgtgctgacggaactgcaacagcgttccgctgctaaccaggcacgtatgaacccgactgcggccatgcctcag BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z