LOCUS       BBa_S04292              3351 bp    DNA     linear       22-Dec-2024
DEFINITION  S04291:B0010
ACCESSION   BBa_S04292
VERSION     BBa_S04292.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..1805
                     /label=BBa_K258100
     stem_loop       1825..1868
                     /label=stem_loop
     misc_feature    1814..1893
                     /label=BBa_B0010
     terminator      1814..1893
                     /label=BBa_B0010
     misc_feature    1906..1909
                     /label=conserved
     RBS             1902..1913
                     /label=BBa_B0034
     CDS             1920..3263
                     /label=BBa_K258101
     misc_feature    1..3263
                     /label=BBa_S04291
     stem_loop       3283..3326
                     /label=stem_loop
     misc_feature    3272..3351
                     /label=BBa_B0010
     terminator      3272..3351
                     /label=BBa_B0010
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaacgctt cctctgaacg cgatcgtagc ctgttcggcg
      121 tgctgcgcca gttccgccgc tccttttggt ctgttggtat cttctctgcg gtgatcaacg
      181 ttctgatgct ggcaccgtct gtttatatgc tgcaggtata tgaccgtgtt ctggcttctg
      241 gtaatggcat caccctgctg atgctgaccc tgctgatggc aggtctgtgc gccttcatgg
      301 gtgcactgga atgggttcgc tccctgctgg tcgttcgcct gggtactcgt atcgatctgg
      361 cactgaacca ggacgtattc aatgcggcct ttgcgcgtaa cctggaagcg ggtgacggtc
      421 gtgcgggtct ggccctgacc gatctgaccc tgctgcgtca gttcatcacg ggtaacgcgc
      481 tgttcgcttt cttcgatgtt ccgtggttcc cgctgttcct gctggtgctg ttcctgctgc
      541 acccatggct gggtatgctg gccctgggcg gtactgtggt gccgggtggt gttggcctgg
      601 cggaaccggc gtccgaccag tctactgctg gtggcagcaa ccagcagtct cagcaggcca
      661 ctcatctggc cgacgctcag ctgcgtaacg cggatgttat tgaagctatg ggcatgctgg
      721 gcaacctgcg tcgtcgttgg ctggcacgcc actatcgttt catttccctg caaaacctgg
      781 caagcgaacg tgctgcagcg gtcggcggtg cgagcaaata ttctcgcatt gctctgcaaa
      841 gcctgatgct gggcctgggt gcactgctgg cgatcgacgg taaaatcacc cctggcatga
      901 tgattgctgg cagcatcctg gttggtcgtg tgctgagccc gatcgatcag ctgatcggtg
      961 tatggaaaca gtggtcttct gcccgtatcg catggcagcg cctgacgcgc ctgattgctg
     1021 cgtatccgcc gcgtccggcg gctatggcac tgcctgcacc ggaaggccac ctgagcgtag
     1081 aacaggtttc tctgcgtacc gctcagggca acacccgcct gcaaaacatc cacttctctc
     1141 tgcaggctgg tgaaaccctg gtgatcctgg gcgcatctgg ttctggcaaa tcctccctgg
     1201 ctcgtctgct ggttggcgcg cagtctccga cccagggtaa agtacgtctg gacggcgcag
     1261 atctgaatca ggttgacaaa aacactttcg gcccgacgat cggctatctg ccgcaggatg
     1321 ttcagctgtt taaaggctcc ctggcagaga acattgcgcg tttcggtgac gccgacccgg
     1381 aaaaagttgt tgctgcggcc aaactggcgg gcgtgcacga actgatcctg tctctgccga
     1441 acggttacga taccgaactg ggcgacggtg gtggtggcct gagcggcggt caacgccagc
     1501 gtatcggtct ggctcgtgcc atgtacggcg atccgtgcct gctgatcctg gacgaaccga
     1561 acgcttccct ggatagcgaa ggcgaccagg ctctgatgca agcaattgtt gcactgcaaa
     1621 aacgtggcgc gaccgtagtt ctgatcaccc accgccctgc actgactacc ctggcccaga
     1681 aaattctgat cctgcacgaa ggtcagcagc agcgtatggg tctggcgcgc gacgtgctga
     1741 cggaactgca acagcgttcc gctgctaacc aggcacgtat gaacccgact gcggccatgc
     1801 ctcagtacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1861 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gaaagaggag aaatactaga
     1921 tgactggcat ggacattacg acgcaggatg aactgaacga ggctgcgatg cgtgaccgtg
     1981 cctcccgtga cgaagaacgt gcactgcgtc tgggttggtg gctggtactg gctggtttcg
     2041 gtggtttcct gctgtgggcg ctgctggctc cactggacaa aggcgtggcg gtacagggca
     2101 atgtcgtggt atctggcaat cgtaaagtta tccagcacat gcagggcggc atcgtggacc
     2161 gcattcaagt taaggacggc gaccgcgtgg ctgcgggtca agttctgctg accctgaatg
     2221 ctgtggacgc acgtaccact agcgaaggtc tgggttccca gtacgatcag ctgatcgcac
     2281 gtgaagctcg tctgctggcg gagcagcgta accagagcag cctggcagcc acgcctcgtc
     2341 tgactcaagc tcgccagcgt ccggaaatgg cagcgatcat tgcactgcaa gaggatctgc
     2401 tgcgttcccg tcagcagagc ctgaaactgg aaattgacgg cgttcgcgcc agcatcgatg
     2461 gcctggaaac cagcctgggc gctctgcaaa aggtaatgtc cagcaaacag tccgagcaag
     2521 ccaccctgtc ccagcagctg caaggtctgc gtccgctggc tgctgacaac tacgttccgc
     2581 gcaacaaaat gctggaaacc gaacgcctgt ttgcgcaagt atctggtgag ctggctcaga
     2641 cttctggtga agtgggtcgt acccgtcgtg atattcagca gcagaaactg cgtattgctc
     2701 agcgtcagca agaatacgac aaagaagtaa actccgaact gtctgacgtt caggccaaac
     2761 tgaacgaagt gatcagccag cgtgaaaaag ccgatttcaa cctggctaac gttcaggtgc
     2821 gcgctccggt cgcgggcacc gtggtagaca tgaaaatctt taccgagggc ggtgtaatcg
     2881 cgccaggtca ggtaatgatg gatattgttc cggaggatca gccgctgctg gtagacggtc
     2941 gcattcctgt agaaatggtt gataaagttt ggagcggtct gccagtagag ctgcaattca
     3001 ctgcgttctc tcagtctacg accccgcgtg ttccgggcac cgtcaccctg ctgtctgcag
     3061 accgtctggt ggatgagaag gatggtaccc cgtactacgg tctgcgcatc caggtttccg
     3121 aggagggtaa acgcagcctg cacggcctgg aaattaaacc tggcatgcct gttcagggct
     3181 tcgtacgtac cggtgaacgt tctttcatta actatctgtt caaaccgctg atggaccgta
     3241 tgcacctggc tctgaccgag gaatactaga gccaggcatc aaataaaacg aaaggctcag
     3301 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct c
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