##gff-version 3
##sequence-region BBa_S04292 1 3351
BBa_S04292	.	terminator	3272	3351	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_S04292	.	engineered_region	3272	3351	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04292	.	stem_loop	3283	3326	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04292	.	engineered_region	1	3263	.	+	0	ID=BBa_S04291;Name=BBa_S04291
BBa_S04292	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04291
BBa_S04292	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04292	.	ribosome_entry_site	1902	1913	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04291
BBa_S04292	.	conserved_region	1906	1909	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04292	.	terminator	1814	1893	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S04291
BBa_S04292	.	engineered_region	1814	1893	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04292	.	stem_loop	1825	1868	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04292	.	CDS	1920	3263	.	+	0	ID=BBa_K258101;Name=BBa_K258101;Parent=BBa_S04291
BBa_S04292	.	CDS	81	1805	.	+	0	ID=BBa_K258100;Name=BBa_K258100;Parent=BBa_S04291
BBa_S04292	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_S04291
BBa_S04292	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_S04292	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_S04292	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_S04292	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_S04292	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
>BBa_S04292
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaacgcttcctctgaacgcgatcgtagcctgttcggcgtgctgcgccagttccgccgctccttttggtctgttggt
atcttctctgcggtgatcaacgttctgatgctggcaccgtctgtttatatgctgcaggtatatgaccgtgttctggctt
ctggtaatggcatcaccctgctgatgctgaccctgctgatggcaggtctgtgcgccttcatgggtgcactggaatgggt
tcgctccctgctggtcgttcgcctgggtactcgtatcgatctggcactgaaccaggacgtattcaatgcggcctttgcg
cgtaacctggaagcgggtgacggtcgtgcgggtctggccctgaccgatctgaccctgctgcgtcagttcatcacgggta
acgcgctgttcgctttcttcgatgttccgtggttcccgctgttcctgctggtgctgttcctgctgcacccatggctggg
tatgctggccctgggcggtactgtggtgccgggtggtgttggcctggcggaaccggcgtccgaccagtctactgctggt
ggcagcaaccagcagtctcagcaggccactcatctggccgacgctcagctgcgtaacgcggatgttattgaagctatgg
gcatgctgggcaacctgcgtcgtcgttggctggcacgccactatcgtttcatttccctgcaaaacctggcaagcgaacg
tgctgcagcggtcggcggtgcgagcaaatattctcgcattgctctgcaaagcctgatgctgggcctgggtgcactgctg
gcgatcgacggtaaaatcacccctggcatgatgattgctggcagcatcctggttggtcgtgtgctgagcccgatcgatc
agctgatcggtgtatggaaacagtggtcttctgcccgtatcgcatggcagcgcctgacgcgcctgattgctgcgtatcc
gccgcgtccggcggctatggcactgcctgcaccggaaggccacctgagcgtagaacaggtttctctgcgtaccgctcag
ggcaacacccgcctgcaaaacatccacttctctctgcaggctggtgaaaccctggtgatcctgggcgcatctggttctg
gcaaatcctccctggctcgtctgctggttggcgcgcagtctccgacccagggtaaagtacgtctggacggcgcagatct
gaatcaggttgacaaaaacactttcggcccgacgatcggctatctgccgcaggatgttcagctgtttaaaggctccctg
gcagagaacattgcgcgtttcggtgacgccgacccggaaaaagttgttgctgcggccaaactggcgggcgtgcacgaac
tgatcctgtctctgccgaacggttacgataccgaactgggcgacggtggtggtggcctgagcggcggtcaacgccagcg
tatcggtctggctcgtgccatgtacggcgatccgtgcctgctgatcctggacgaaccgaacgcttccctggatagcgaa
ggcgaccaggctctgatgcaagcaattgttgcactgcaaaaacgtggcgcgaccgtagttctgatcacccaccgccctg
cactgactaccctggcccagaaaattctgatcctgcacgaaggtcagcagcagcgtatgggtctggcgcgcgacgtgct
gacggaactgcaacagcgttccgctgctaaccaggcacgtatgaacccgactgcggccatgcctcagtactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagaaagaggagaaatactagatgactggcatggacattacgacgcaggatgaactgaacgaggctgcgatgcgtga
ccgtgcctcccgtgacgaagaacgtgcactgcgtctgggttggtggctggtactggctggtttcggtggtttcctgctg
tgggcgctgctggctccactggacaaaggcgtggcggtacagggcaatgtcgtggtatctggcaatcgtaaagttatcc
agcacatgcagggcggcatcgtggaccgcattcaagttaaggacggcgaccgcgtggctgcgggtcaagttctgctgac
cctgaatgctgtggacgcacgtaccactagcgaaggtctgggttcccagtacgatcagctgatcgcacgtgaagctcgt
ctgctggcggagcagcgtaaccagagcagcctggcagccacgcctcgtctgactcaagctcgccagcgtccggaaatgg
cagcgatcattgcactgcaagaggatctgctgcgttcccgtcagcagagcctgaaactggaaattgacggcgttcgcgc
cagcatcgatggcctggaaaccagcctgggcgctctgcaaaaggtaatgtccagcaaacagtccgagcaagccaccctg
tcccagcagctgcaaggtctgcgtccgctggctgctgacaactacgttccgcgcaacaaaatgctggaaaccgaacgcc
tgtttgcgcaagtatctggtgagctggctcagacttctggtgaagtgggtcgtacccgtcgtgatattcagcagcagaa
actgcgtattgctcagcgtcagcaagaatacgacaaagaagtaaactccgaactgtctgacgttcaggccaaactgaac
gaagtgatcagccagcgtgaaaaagccgatttcaacctggctaacgttcaggtgcgcgctccggtcgcgggcaccgtgg
tagacatgaaaatctttaccgagggcggtgtaatcgcgccaggtcaggtaatgatggatattgttccggaggatcagcc
gctgctggtagacggtcgcattcctgtagaaatggttgataaagtttggagcggtctgccagtagagctgcaattcact
gcgttctctcagtctacgaccccgcgtgttccgggcaccgtcaccctgctgtctgcagaccgtctggtggatgagaagg
atggtaccccgtactacggtctgcgcatccaggtttccgaggagggtaaacgcagcctgcacggcctggaaattaaacc
tggcatgcctgttcagggcttcgtacgtaccggtgaacgttctttcattaactatctgttcaaaccgctgatggaccgt
atgcacctggctctgaccgaggaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt
cgttttatctgttgtttgtcggtgaacgctctc