LOCUS       BBa_S04303              2315 bp    DNA     linear       16-Jun-2024
DEFINITION  S04302:S04301
ACCESSION   BBa_S04303
VERSION     BBa_S04303.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    1108..1146
                     /label=LVA
     CDS             19..1146
                     /label=lacI-LVA
     misc_feature    19..1146
                     /label=BBa_C0012
     misc_feature    1147..1171
                     /label=Help:Barcodes
     CDS             19..1146
                     /label=BBa_C0012
     stem_loop       1191..1234
                     /label=stem_loop
     misc_feature    1180..1259
                     /label=BBa_B0010
     terminator      1180..1259
                     /label=BBa_B0010
     stem_loop       1275..1294
                     /label=T7 TE
     misc_feature    1301..1301
                     /label=stop
     polyA_site      1295..1308
                     /label=polya
     misc_feature    1268..1308
                     /label=BBa_B0012
     terminator      1268..1308
                     /label=BBa_B0012
     terminator      1180..1308
                     /label=BBa_B0015
     misc_feature    1319..1335
                     /label=lac O1
     promoter        1336..1341
                     /label=-35
     misc_feature    1342..1358
                     /label=lac O1
     promoter        1359..1364
                     /label=-10
     misc_feature    1317..1370
                     /label=BBa_R0011
     promoter        1317..1370
                     /label=BBa_R0011
     misc_feature    1..1371
                     /label=BBa_Q04121
     misc_feature    1384..1387
                     /label=conserved
     RBS             1380..1391
                     /label=BBa_B0034
     misc_feature    1..1391
                     /label=BBa_S04302
     CDS             1398..2114
                     /label=lasR
     misc_feature    2115..2147
                     /label=LVA
     misc_feature    1877..1877
                     /label=G
     misc_feature    2154..2178
                     /label=Help:Barcodes
     CDS             1398..2178
                     /label=BBa_C0079
     stem_loop       2198..2241
                     /label=stem_loop
     misc_feature    2187..2266
                     /label=BBa_B0010
     terminator      2187..2266
                     /label=BBa_B0010
     stem_loop       2282..2301
                     /label=T7 TE
     misc_feature    2308..2308
                     /label=stop
     polyA_site      2302..2315
                     /label=polya
     misc_feature    2275..2315
                     /label=BBa_B0012
     terminator      2275..2315
                     /label=BBa_B0012
     terminator      2187..2315
                     /label=BBa_B0015
     CDS             1398..2315
                     /label=BBa_S04301
ORIGIN
        1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
       61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
      121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
      181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
      241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
      301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
      361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
      421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
      481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
      541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
      601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
      661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
      721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
      781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg
      841 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
      901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
      961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
     1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct
     1141 taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa
     1201 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1261 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaatt
     1321 gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac atactagaga
     1381 aagaggagaa atactagatg gccttggttg acggttttct tgagctggaa cgctcaagtg
     1441 gaaaattgga gtggagcgcc atcctccaga agatggcgag cgaccttgga ttctcgaaga
     1501 tcctgttcgg cctgttgcct aaggacagcc aggactacga gaacgccttc atcgtcggca
     1561 actacccggc cgcctggcgc gagcattacg accgggctgg ctacgcgcgg gtcgacccga
     1621 cggtcagtca ctgtacccag agcgtactgc cgattttctg ggaaccgtcc atctaccaga
     1681 cgcgaaagca gcacgagttc ttcgaggaag cctcggccgc cggcctggtg tatgggctga
     1741 ccatgccgct gcatggtgct cgcggcgaac tcggcgcgct gagcctcagc gtggaagcgg
     1801 aaaaccgggc cgaggccaac cgtttcatag agtcggtcct gccgaccctg tggatgctca
     1861 aggactacgc actgcaaagc ggtgccggac tggccttcga acatccggtc agcaaaccgg
     1921 tggttctgac cagccgggag aaggaagtgt tgcagtggtg cgccatcggc aagaccagtt
     1981 gggagatatc ggttatctgc aactgctcgg aagccaatgt gaacttccat atgggaaata
     2041 ttcggcggaa gttcggtgtg acctcccgcc gcgtagcggc cattatggcc gttaatttgg
     2101 gtcttattac tctcgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat
     2161 agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     2221 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     2281 tggctcacct tcgggtgggc ctttctgcgt ttata
//