##gff-version 3
##sequence-region BBa_S04305 1 1705
BBa_S04305	.	engineered_region	1	819	.	+	0	ID=BBa_S04304;Name=BBa_S04304
BBa_S04305	.	engineered_region	135	819	.	+	0	ID=BBa_S03154;Name=BBa_S03154;Parent=BBa_S04304
BBa_S04305	.	ribosome_entry_site	135	146	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03154
BBa_S04305	.	conserved_region	139	142	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_S04305	.	CDS	153	794	.	+	0	ID=BBa_C0078;Name=BBa_C0078;Parent=BBa_S03154
BBa_S04305	.	CDS	153	755	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_S04305	.	engineered_tag	795	819	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
BBa_S04305	.	tag	756	788	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
BBa_S04305	.	promoter	1	126	.	+	0	ID=BBa_K091146;Name=BBa_K091146;Parent=BBa_S04304
BBa_S04305	.	sequence_feature	86	91	.	+	0	ID=annotation1967357;Name=-35;Parent=BBa_K091146
BBa_S04305	.	start_codon	125	125	.	+	0	ID=annotation1967358;Name=Transcriptional Start;Parent=BBa_K091146
BBa_S04305	.	non_covalent_binding_site	94	113	.	+	0	ID=annotation1967355;Name=LuxR;Parent=BBa_K091146
BBa_S04305	.	non_covalent_binding_site	75	93	.	+	0	ID=annotation1967354;Name=LasR;Parent=BBa_K091146
BBa_S04305	.	sequence_feature	113	118	.	+	0	ID=annotation1967356;Name=-10;Parent=BBa_K091146
BBa_S04305	.	engineered_region	828	1705	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_S04305	.	ribosome_entry_site	828	842	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_S04305	.	engineered_region	828	842	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_S04305	.	ribosome_entry_site	828	842	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_S04305	.	ribosome_entry_site	835	839	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_S04305	.	CDS	849	1568	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_S04305	.	CDS	849	1568	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_S04305	.	terminator	1577	1656	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_S04305	.	stem_loop	1588	1631	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_S04305	.	engineered_region	1577	1656	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_S04305	.	terminator	1665	1705	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_S04305	.	engineered_region	1665	1705	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_S04305	.	stop_codon	1698	1698	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_S04305	.	polyA_site	1692	1705	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_S04305	.	stem_loop	1672	1691	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_S04305
tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatcta
tctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagaaagaggagaaatactagatgatc
gttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaag
aacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgct
gatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaa
aacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatca
actccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgca
gaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcc
cgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcg
ctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgctta
ataactctgatagtgctagtgtagatctctactagagattaaagaggagaaatactagatgcgtaaaggagaagaactt
ttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtg
aaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacact
tgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagt
gccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtca
agtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggaca
caaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttc
aaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggcc
ctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacat
ggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttata