LOCUS       BBa_S04307              2157 bp    DNA     linear       29-Apr-2024
DEFINITION  K091225:P0440
ACCESSION   BBa_S04307
VERSION     BBa_S04307.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    1246..1284
                     /label=LVA
     CDS             157..1284
                     /label=lacI-LVA
     misc_feature    157..1284
                     /label=BBa_C0012
     misc_feature    1285..1309
                     /label=Help:Barcodes
     CDS             157..1284
                     /label=BBa_C0012
     misc_feature    139..1309
                     /label=BBa_S0100
     promoter        1..1309
                     /label=BBa_K091225
     misc_feature    1322..1325
                     /label=conserved
     RBS             1318..1329
                     /label=BBa_B0034
     CDS             1339..1955
                     /label=tetR
     misc_feature    1956..1989
                     /label=SsrA
     misc_feature    1996..2020
                     /label=Help:Barcodes
     CDS             1336..1995
                     /label=BBa_C0040
     stem_loop       2040..2083
                     /label=stem_loop
     misc_feature    2029..2108
                     /label=BBa_B0010
     terminator      2029..2108
                     /label=BBa_B0010
     stem_loop       2124..2143
                     /label=T7 TE
     misc_feature    2150..2150
                     /label=stop
     polyA_site      2144..2157
                     /label=polya
     misc_feature    2117..2157
                     /label=BBa_B0012
     terminator      2117..2157
                     /label=BBa_B0012
     misc_feature    1318..2157
                     /label=BBa_P0440
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg
      181 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac
      241 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg
      301 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc
      361 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct
      421 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa
      481 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac
      541 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg
      601 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac
      661 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta
      721 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc
      781 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt
      841 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc
      901 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt
      961 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg
     1021 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg
     1081 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa
     1141 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1201 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa
     1261 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagaaa
     1321 gaggagaaat actagatgtc cagattagat aaaagtaaag tgattaacag cgcattagag
     1381 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt
     1441 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta
     1501 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg
     1561 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat
     1621 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat
     1681 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc
     1741 gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa
     1801 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa
     1861 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc
     1921 ggattagaaa aacaacttaa atgtgaaagt gggtccgctg caaacgacga aaactacgct
     1981 ttagtagctt aataacactg atagtgctag tgtagatcac tactagagcc aggcatcaaa
     2041 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     2101 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//